In Vitro Study of Biocontrol Potential of Rhizospheric Pseudomonas aeruginosa against Pathogenic Fungi of Saffron (Crocus sativus L.)

Plant rhizosphere soil contains a large number of plant-growth promoting rhizobacteria, which can not only resist the invasion of pathogenic microorganisms and protect plants from damage, but also promote the growth and development of plants. In this study, Pseudomonas aeruginosa...

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Autores principales:	Shuang Hu, Xingxing Wang, Wenjing Sun, Lili Wang, Wankui Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pseudomonas aeruginosa</i> saffron rhizosphere antagonistic activity biological control PGPR Medicine R
Acceso en línea:	https://doaj.org/article/9505e47f91e84a57ae4ecaa9ebefa0e1
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spelling	oai:doaj.org-article:9505e47f91e84a57ae4ecaa9ebefa0e12021-11-25T18:38:09ZIn Vitro Study of Biocontrol Potential of Rhizospheric <i>Pseudomonas aeruginosa</i> against Pathogenic Fungi of Saffron (<i>Crocus sativus</i> L.)10.3390/pathogens101114232076-0817https://doaj.org/article/9505e47f91e84a57ae4ecaa9ebefa0e12021-11-01T00:00:00Zhttps://www.mdpi.com/2076-0817/10/11/1423https://doaj.org/toc/2076-0817Plant rhizosphere soil contains a large number of plant-growth promoting rhizobacteria, which can not only resist the invasion of pathogenic microorganisms and protect plants from damage, but also promote the growth and development of plants. In this study, <i>Pseudomonas aeruginosa</i> strain YY322, isolated and screened from the rhizosphere soil of saffron (<i>Crocus sativus</i> L.), was found through a plate confrontation experiment to show highly effectual and obvious antagonistic activity against the pathogens of saffron, including <i>Fusarium oxysporum</i>, <i>Fusarium solani</i>, <i>Penicillium citreosulfuratum</i>, <i>Penicillium citrinum</i> and <i>Stromatinia gladioli</i>. In addition, the volatile organic compounds of strain YY322 had great antagonistic activity against these pathogens. Observation under a scanning electron microscope and transmission electron microscope reflected that strain YY322 had a significant effect on the hyphae and conidia of <i>F. oxysporum</i> and <i>F. solani</i>. Through the detection of degrading enzymes, it was found that <i>P. aeruginosa</i> can secrete protease and glucanase. The plant growth promoting performance was evaluated, finding that strain YY322 had the functions of dissolving phosphorus, fixing nitrogen, producing siderophore and producing NH<sub>3</sub>. In addition, whole genome sequencing analysis indicated that the YY322 genome is comprised of a 6,382,345-bp circular chromosome, containing 5809 protein-coding genes and 151 RNA genes. The <i>P. aeruginosa</i> YY322 genome encodes genes related to phenazine (<i>phzABDEFGIMRS</i>), hydrogen cyanide(HCN) (<i>hcnABC</i>), surfactin (<i>srfAA</i>), salicylate (<i>pchA</i>), biofilm formation (<i>flgBCDEFGHIJKL</i>, <i>motAB</i>, <i>efp</i>, <i>hfq</i>), and colonization (<i>minCDE</i>, <i>yjbB</i>, <i>lysC</i>). These results collectively indicated the role of <i>P. aeruginosa</i> YY322 in plant growth enhancement and biocontrol mechanisms. All in all, this study provides a theoretical basis for <i>P. aeruginosa</i> as the PGPR of saffron, paving the way for the subsequent development and utilization of microbial fertilizer.Shuang HuXingxing WangWenjing SunLili WangWankui LiMDPI AGarticle<i>Pseudomonas aeruginosa</i>saffronrhizosphereantagonistic activitybiological controlPGPRMedicineRENPathogens, Vol 10, Iss 1423, p 1423 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pseudomonas aeruginosa</i> saffron rhizosphere antagonistic activity biological control PGPR Medicine R
spellingShingle	<i>Pseudomonas aeruginosa</i> saffron rhizosphere antagonistic activity biological control PGPR Medicine R Shuang Hu Xingxing Wang Wenjing Sun Lili Wang Wankui Li In Vitro Study of Biocontrol Potential of Rhizospheric <i>Pseudomonas aeruginosa</i> against Pathogenic Fungi of Saffron (<i>Crocus sativus</i> L.)
description	Plant rhizosphere soil contains a large number of plant-growth promoting rhizobacteria, which can not only resist the invasion of pathogenic microorganisms and protect plants from damage, but also promote the growth and development of plants. In this study, <i>Pseudomonas aeruginosa</i> strain YY322, isolated and screened from the rhizosphere soil of saffron (<i>Crocus sativus</i> L.), was found through a plate confrontation experiment to show highly effectual and obvious antagonistic activity against the pathogens of saffron, including <i>Fusarium oxysporum</i>, <i>Fusarium solani</i>, <i>Penicillium citreosulfuratum</i>, <i>Penicillium citrinum</i> and <i>Stromatinia gladioli</i>. In addition, the volatile organic compounds of strain YY322 had great antagonistic activity against these pathogens. Observation under a scanning electron microscope and transmission electron microscope reflected that strain YY322 had a significant effect on the hyphae and conidia of <i>F. oxysporum</i> and <i>F. solani</i>. Through the detection of degrading enzymes, it was found that <i>P. aeruginosa</i> can secrete protease and glucanase. The plant growth promoting performance was evaluated, finding that strain YY322 had the functions of dissolving phosphorus, fixing nitrogen, producing siderophore and producing NH<sub>3</sub>. In addition, whole genome sequencing analysis indicated that the YY322 genome is comprised of a 6,382,345-bp circular chromosome, containing 5809 protein-coding genes and 151 RNA genes. The <i>P. aeruginosa</i> YY322 genome encodes genes related to phenazine (<i>phzABDEFGIMRS</i>), hydrogen cyanide(HCN) (<i>hcnABC</i>), surfactin (<i>srfAA</i>), salicylate (<i>pchA</i>), biofilm formation (<i>flgBCDEFGHIJKL</i>, <i>motAB</i>, <i>efp</i>, <i>hfq</i>), and colonization (<i>minCDE</i>, <i>yjbB</i>, <i>lysC</i>). These results collectively indicated the role of <i>P. aeruginosa</i> YY322 in plant growth enhancement and biocontrol mechanisms. All in all, this study provides a theoretical basis for <i>P. aeruginosa</i> as the PGPR of saffron, paving the way for the subsequent development and utilization of microbial fertilizer.
format	article
author	Shuang Hu Xingxing Wang Wenjing Sun Lili Wang Wankui Li
author_facet	Shuang Hu Xingxing Wang Wenjing Sun Lili Wang Wankui Li
author_sort	Shuang Hu
title	In Vitro Study of Biocontrol Potential of Rhizospheric <i>Pseudomonas aeruginosa</i> against Pathogenic Fungi of Saffron (<i>Crocus sativus</i> L.)
title_short	In Vitro Study of Biocontrol Potential of Rhizospheric <i>Pseudomonas aeruginosa</i> against Pathogenic Fungi of Saffron (<i>Crocus sativus</i> L.)
title_full	In Vitro Study of Biocontrol Potential of Rhizospheric <i>Pseudomonas aeruginosa</i> against Pathogenic Fungi of Saffron (<i>Crocus sativus</i> L.)
title_fullStr	In Vitro Study of Biocontrol Potential of Rhizospheric <i>Pseudomonas aeruginosa</i> against Pathogenic Fungi of Saffron (<i>Crocus sativus</i> L.)
title_full_unstemmed	In Vitro Study of Biocontrol Potential of Rhizospheric <i>Pseudomonas aeruginosa</i> against Pathogenic Fungi of Saffron (<i>Crocus sativus</i> L.)
title_sort	in vitro study of biocontrol potential of rhizospheric <i>pseudomonas aeruginosa</i> against pathogenic fungi of saffron (<i>crocus sativus</i> l.)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/9505e47f91e84a57ae4ecaa9ebefa0e1
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In Vitro Study of Biocontrol Potential of Rhizospheric <i>Pseudomonas aeruginosa</i> against Pathogenic Fungi of Saffron (<i>Crocus sativus</i> L.)

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