Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens

Sankar Renu, 1 Ashley D Markazi, 2 Santosh Dhakal, 1 Yashavanth S Lakshmanappa, 1 Revathi Shanmugasundaram, 2 Ramesh K Selvaraj, 3 Gourapura J Renukaradhya 1 1Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA and Department of Veterinary...

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Autores principales:	Renu S, Markazi AD, Dhakal S, Lakshmanappa YS, Shanmugasundaram R, Selvaraj RK, Renukaradhya GJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	chickens chitosan nanoparticle salmonella antigens oral delivery mucosal immune response. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/950d3f98e28e41859f08befd7c6002f9
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spelling	oai:doaj.org-article:950d3f98e28e41859f08befd7c6002f92021-12-02T09:07:46ZOral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens1178-2013https://doaj.org/article/950d3f98e28e41859f08befd7c6002f92020-02-01T00:00:00Zhttps://www.dovepress.com/oral-deliverable-mucoadhesive-chitosan-salmonella-subunit-nanovaccine--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sankar Renu, 1 Ashley D Markazi, 2 Santosh Dhakal, 1 Yashavanth S Lakshmanappa, 1 Revathi Shanmugasundaram, 2 Ramesh K Selvaraj, 3 Gourapura J Renukaradhya 1 1Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA and Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA; 2Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Columbus, OH, USA; 3Department of Poultry Science, University of Georgia, Athens, GA 30602, USACorrespondence: Gourapura J RenukaradhyaFood Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USATel +1 330-263-3748Fax +1 330-263-3677Email gourapura.1@osu.eduRamesh K SelvarajDepartment of Poultry Science, University of Georgia, Athens, GA 30602, USAEmail selvaraj@uga.eduPurpose: Salmonellosis in poultry is a serious economic burden. A major concern is the public health hazard caused by consumption of Salmonella-contaminated poultry products. Currently used Salmonella vaccines are ineffective in combating poultry Salmonellosis warranting the need of a potent vaccine, especially an oral vaccine that can elicit robust local intestinal immunity.Materials and Methods: A Salmonella subunit chitosan nanoparticles (NPs)-based vaccine was prepared that contained immunogenic outer membrane proteins (OMPs) and -flagellin (F) protein (OMPs-F-CS NPs). OMPs-F-CS NPs were administered as an oral vaccine in layer chickens and the resultant humoral and cell-mediated immune responses and localization of NPs were examined using standard detection methods.Results: We demonstrated targeting of surface F-protein coated chitosan NPs to immune cells when delivered orally to layer chickens, the particles were localized in ileal Peyer’s patches. The OMPs-F-CS NPs vaccinated layer chickens had significantly higher OMPs-specific mucosal IgA production and lymphocyte proliferation response. The candidate vaccine increased the expression of toll-like receptor (TLR)-2, TLR-4, IFN-γ, TGF-ß and IL-4 mRNA expression in chicken cecal tonsils.Conclusion: Our study demonstrated that the chitosan-based oral Salmonella nanovaccine targets immune cells of chickens and induced antigen-specific B and T cell responses. This candidate oral Salmonella nanovaccine has the potential to mitigate Salmonellosis in poultry.Keywords: chickens, chitosan nanoparticle, Salmonella antigens, oral delivery, mucosal immune responseRenu SMarkazi ADDhakal SLakshmanappa YSShanmugasundaram RSelvaraj RKRenukaradhya GJDove Medical Pressarticlechickenschitosan nanoparticlesalmonella antigensoral deliverymucosal immune response.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 761-777 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	chickens chitosan nanoparticle salmonella antigens oral delivery mucosal immune response. Medicine (General) R5-920
spellingShingle	chickens chitosan nanoparticle salmonella antigens oral delivery mucosal immune response. Medicine (General) R5-920 Renu S Markazi AD Dhakal S Lakshmanappa YS Shanmugasundaram R Selvaraj RK Renukaradhya GJ Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens
description	Sankar Renu, 1 Ashley D Markazi, 2 Santosh Dhakal, 1 Yashavanth S Lakshmanappa, 1 Revathi Shanmugasundaram, 2 Ramesh K Selvaraj, 3 Gourapura J Renukaradhya 1 1Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA and Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA; 2Department of Animal Sciences, Ohio Agricultural Research and Development Center, The Ohio State University, Columbus, OH, USA; 3Department of Poultry Science, University of Georgia, Athens, GA 30602, USACorrespondence: Gourapura J RenukaradhyaFood Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USATel +1 330-263-3748Fax +1 330-263-3677Email gourapura.1@osu.eduRamesh K SelvarajDepartment of Poultry Science, University of Georgia, Athens, GA 30602, USAEmail selvaraj@uga.eduPurpose: Salmonellosis in poultry is a serious economic burden. A major concern is the public health hazard caused by consumption of Salmonella-contaminated poultry products. Currently used Salmonella vaccines are ineffective in combating poultry Salmonellosis warranting the need of a potent vaccine, especially an oral vaccine that can elicit robust local intestinal immunity.Materials and Methods: A Salmonella subunit chitosan nanoparticles (NPs)-based vaccine was prepared that contained immunogenic outer membrane proteins (OMPs) and -flagellin (F) protein (OMPs-F-CS NPs). OMPs-F-CS NPs were administered as an oral vaccine in layer chickens and the resultant humoral and cell-mediated immune responses and localization of NPs were examined using standard detection methods.Results: We demonstrated targeting of surface F-protein coated chitosan NPs to immune cells when delivered orally to layer chickens, the particles were localized in ileal Peyer’s patches. The OMPs-F-CS NPs vaccinated layer chickens had significantly higher OMPs-specific mucosal IgA production and lymphocyte proliferation response. The candidate vaccine increased the expression of toll-like receptor (TLR)-2, TLR-4, IFN-γ, TGF-ß and IL-4 mRNA expression in chicken cecal tonsils.Conclusion: Our study demonstrated that the chitosan-based oral Salmonella nanovaccine targets immune cells of chickens and induced antigen-specific B and T cell responses. This candidate oral Salmonella nanovaccine has the potential to mitigate Salmonellosis in poultry.Keywords: chickens, chitosan nanoparticle, Salmonella antigens, oral delivery, mucosal immune response
format	article
author	Renu S Markazi AD Dhakal S Lakshmanappa YS Shanmugasundaram R Selvaraj RK Renukaradhya GJ
author_facet	Renu S Markazi AD Dhakal S Lakshmanappa YS Shanmugasundaram R Selvaraj RK Renukaradhya GJ
author_sort	Renu S
title	Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens
title_short	Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens
title_full	Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens
title_fullStr	Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens
title_full_unstemmed	Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens
title_sort	oral deliverable mucoadhesive chitosan-salmonella subunit nanovaccine for layer chickens
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/950d3f98e28e41859f08befd7c6002f9
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Oral Deliverable Mucoadhesive Chitosan-Salmonella Subunit Nanovaccine for Layer Chickens

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