Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation and fatty acid content

Abstract. Perdana BA, Dharma A, Zakaria IJ, Syafrizayanti. 2021. Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation, and fatty acid content. Biodiversitas 22: 505-511. Microalgae have capability to produce fatty acid for biofuel, drugs, and nutraceutical foods deve...

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Autores principales:	Bayu Afnovandra Perdana, Abdi Dharma, Indra Junaidi Zakaria, Syafrizayanti SYAFRIZAYANTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	microalgae fatty acids lipid microalgae isolation molecular identification Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/950e2a1bf4a14504addc3580713c99af
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spelling	oai:doaj.org-article:950e2a1bf4a14504addc3580713c99af2021-11-22T00:54:56ZFreshwater pond microalgae for biofuel: Strain isolation, identification, cultivation and fatty acid content1412-033X2085-472210.13057/biodiv/d220201https://doaj.org/article/950e2a1bf4a14504addc3580713c99af2021-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5821https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Perdana BA, Dharma A, Zakaria IJ, Syafrizayanti. 2021. Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation, and fatty acid content. Biodiversitas 22: 505-511. Microalgae have capability to produce fatty acid for biofuel, drugs, and nutraceutical foods development. This study was carried out to obtain a new strain candidate for fatty acid production. The methods were used in this study include isolation of microalgae species from freshwater ponds of Andalas University, Padang, Indonesia. Molecular identification of microalgae was carried out with specific 18S rRNA primer, F-P73, and R-P47. Microalgae growth was measured by cell density and optical density method using various wavelengths (400, 500, and 680 nm). Total lipid was extracted using Bligh & Dyer method. Fatty acid analyses were conducted using gas chromatography-mass spectroscopy. Microalgae were isolated i.e Chlorella emersonii MAUA001, Mychonastes rotundus MAUA002, Scenedesmus dimorphus MAUA003, and Scenedesmus armatus MAUA004. The result exhibited M. rotundus was the highest lipid content, it was about 28.8% biomass weight. Fatty acid profiles of microalgae were dominated by monounsaturated (MUFA) and saturated fatty acid (SFA). The highest content of fatty acid species found in C. emersonii with octadecenoic acid (C18:1) was 47.74% total lipid. This work showed that C. emersonii has potential as biodiesel due to high saturated fatty acid.Bayu Afnovandra PerdanaAbdi DharmaIndra Junaidi ZakariaSyafrizayanti SYAFRIZAYANTIMBI & UNS Soloarticlemicroalgaefatty acidslipidmicroalgae isolationmolecular identificationBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microalgae fatty acids lipid microalgae isolation molecular identification Biology (General) QH301-705.5
spellingShingle	microalgae fatty acids lipid microalgae isolation molecular identification Biology (General) QH301-705.5 Bayu Afnovandra Perdana Abdi Dharma Indra Junaidi Zakaria Syafrizayanti SYAFRIZAYANTI Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation and fatty acid content
description	Abstract. Perdana BA, Dharma A, Zakaria IJ, Syafrizayanti. 2021. Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation, and fatty acid content. Biodiversitas 22: 505-511. Microalgae have capability to produce fatty acid for biofuel, drugs, and nutraceutical foods development. This study was carried out to obtain a new strain candidate for fatty acid production. The methods were used in this study include isolation of microalgae species from freshwater ponds of Andalas University, Padang, Indonesia. Molecular identification of microalgae was carried out with specific 18S rRNA primer, F-P73, and R-P47. Microalgae growth was measured by cell density and optical density method using various wavelengths (400, 500, and 680 nm). Total lipid was extracted using Bligh & Dyer method. Fatty acid analyses were conducted using gas chromatography-mass spectroscopy. Microalgae were isolated i.e Chlorella emersonii MAUA001, Mychonastes rotundus MAUA002, Scenedesmus dimorphus MAUA003, and Scenedesmus armatus MAUA004. The result exhibited M. rotundus was the highest lipid content, it was about 28.8% biomass weight. Fatty acid profiles of microalgae were dominated by monounsaturated (MUFA) and saturated fatty acid (SFA). The highest content of fatty acid species found in C. emersonii with octadecenoic acid (C18:1) was 47.74% total lipid. This work showed that C. emersonii has potential as biodiesel due to high saturated fatty acid.
format	article
author	Bayu Afnovandra Perdana Abdi Dharma Indra Junaidi Zakaria Syafrizayanti SYAFRIZAYANTI
author_facet	Bayu Afnovandra Perdana Abdi Dharma Indra Junaidi Zakaria Syafrizayanti SYAFRIZAYANTI
author_sort	Bayu Afnovandra Perdana
title	Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation and fatty acid content
title_short	Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation and fatty acid content
title_full	Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation and fatty acid content
title_fullStr	Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation and fatty acid content
title_full_unstemmed	Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation and fatty acid content
title_sort	freshwater pond microalgae for biofuel: strain isolation, identification, cultivation and fatty acid content
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/950e2a1bf4a14504addc3580713c99af
work_keys_str_mv	AT bayuafnovandraperdana freshwaterpondmicroalgaeforbiofuelstrainisolationidentificationcultivationandfattyacidcontent AT abdidharma freshwaterpondmicroalgaeforbiofuelstrainisolationidentificationcultivationandfattyacidcontent AT indrajunaidizakaria freshwaterpondmicroalgaeforbiofuelstrainisolationidentificationcultivationandfattyacidcontent AT syafrizayantisyafrizayanti freshwaterpondmicroalgaeforbiofuelstrainisolationidentificationcultivationandfattyacidcontent
_version_	1718418473676177408

Freshwater pond microalgae for biofuel: Strain isolation, identification, cultivation and fatty acid content

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