Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes

Abstract Soybean is an important source of protein, oil and carbohydrates, as well as other beneficial nutrients. A major function of proteins in nutrition is to supply adequate amounts of amino acids. Although they are essential for human nutrition, the sulfur-containing amino acids cysteine (Cys)...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sidiki Malle, Milad Eskandari, Malcolm Morrison, François Belzile
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/951022e99d8e48ea835f61557e848eef
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:951022e99d8e48ea835f61557e848eef
record_format	dspace
spelling	oai:doaj.org-article:951022e99d8e48ea835f61557e848eef2021-12-02T12:33:04ZGenome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes10.1038/s41598-020-78907-w2045-2322https://doaj.org/article/951022e99d8e48ea835f61557e848eef2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78907-whttps://doaj.org/toc/2045-2322Abstract Soybean is an important source of protein, oil and carbohydrates, as well as other beneficial nutrients. A major function of proteins in nutrition is to supply adequate amounts of amino acids. Although they are essential for human nutrition, the sulfur-containing amino acids cysteine (Cys) and methionine (Met) are often limited and the genetic control of their content in soybean seeds is poorly characterized. This study aimed to characterize the phenotypic variation and identify quantitative trait loci (QTL) associated with Cys and Met content in a core set of 137 soybean lines, representative of the genetic diversity among Canadian short-season soybean, spanning maturity groups 000-II (MG000-II). Significant phenotypic differences were found among these lines for Cys, Met and Cys + Met content. Using both a mixed linear model and six multi-locus methods with a catalogue of 2.18 M SNPs, we report a total of nine QTLs and seventeen QTNs of which seven comprise promising candidate genes. This work allowed us to reproducibly detect multiple novel loci associated with sulfur-containing amino acid content. The markers and genes identified in this study may be useful for soybean genetic improvement aiming to increase Cys and Met content.Sidiki MalleMilad EskandariMalcolm MorrisonFrançois BelzileNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sidiki Malle Milad Eskandari Malcolm Morrison François Belzile Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes
description	Abstract Soybean is an important source of protein, oil and carbohydrates, as well as other beneficial nutrients. A major function of proteins in nutrition is to supply adequate amounts of amino acids. Although they are essential for human nutrition, the sulfur-containing amino acids cysteine (Cys) and methionine (Met) are often limited and the genetic control of their content in soybean seeds is poorly characterized. This study aimed to characterize the phenotypic variation and identify quantitative trait loci (QTL) associated with Cys and Met content in a core set of 137 soybean lines, representative of the genetic diversity among Canadian short-season soybean, spanning maturity groups 000-II (MG000-II). Significant phenotypic differences were found among these lines for Cys, Met and Cys + Met content. Using both a mixed linear model and six multi-locus methods with a catalogue of 2.18 M SNPs, we report a total of nine QTLs and seventeen QTNs of which seven comprise promising candidate genes. This work allowed us to reproducibly detect multiple novel loci associated with sulfur-containing amino acid content. The markers and genes identified in this study may be useful for soybean genetic improvement aiming to increase Cys and Met content.
format	article
author	Sidiki Malle Milad Eskandari Malcolm Morrison François Belzile
author_facet	Sidiki Malle Milad Eskandari Malcolm Morrison François Belzile
author_sort	Sidiki Malle
title	Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes
title_short	Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes
title_full	Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes
title_fullStr	Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes
title_full_unstemmed	Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes
title_sort	genome-wide association identifies several qtls controlling cysteine and methionine content in soybean seed including some promising candidate genes
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/951022e99d8e48ea835f61557e848eef
work_keys_str_mv	AT sidikimalle genomewideassociationidentifiesseveralqtlscontrollingcysteineandmethioninecontentinsoybeanseedincludingsomepromisingcandidategenes AT miladeskandari genomewideassociationidentifiesseveralqtlscontrollingcysteineandmethioninecontentinsoybeanseedincludingsomepromisingcandidategenes AT malcolmmorrison genomewideassociationidentifiesseveralqtlscontrollingcysteineandmethioninecontentinsoybeanseedincludingsomepromisingcandidategenes AT francoisbelzile genomewideassociationidentifiesseveralqtlscontrollingcysteineandmethioninecontentinsoybeanseedincludingsomepromisingcandidategenes
_version_	1718393873826316288

Genome-wide association identifies several QTLs controlling cysteine and methionine content in soybean seed including some promising candidate genes

Ejemplares similares