Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels

Abstract Differentiation of stem cells to chondrocytes in vitro usually results in a heterogeneous phenotype. This is evident in the often detected over expression of type X collagen which, in hyaline cartilage structure is not characteristic of the mid-zone but of the deep-zone ossifying tissue. Me...

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Autores principales:	Enateri V. Alakpa, Vineetha Jayawarna, Karl E. V. Burgess, Christopher C. West, Bruno Péault, Rein V. Ulijn, Matthew J. Dalby
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95134f7e2eff4a20a241be0b0bcaf930
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spelling	oai:doaj.org-article:95134f7e2eff4a20a241be0b0bcaf9302021-12-02T15:05:27ZImproving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels10.1038/s41598-017-07255-z2045-2322https://doaj.org/article/95134f7e2eff4a20a241be0b0bcaf9302017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07255-zhttps://doaj.org/toc/2045-2322Abstract Differentiation of stem cells to chondrocytes in vitro usually results in a heterogeneous phenotype. This is evident in the often detected over expression of type X collagen which, in hyaline cartilage structure is not characteristic of the mid-zone but of the deep-zone ossifying tissue. Methods to better match cartilage developed in vitro to characteristic in vivo features are therefore highly desirable in regenerative medicine. This study compares phenotype characteristics between pericytes, obtained from human adipose tissue, differentiated using diphenylalanine/serine (F2/S) peptide hydrogels with the more widely used chemical induced method for chondrogenesis. Significantly higher levels of type II collagen were noted when pericytes undergo chondrogenesis in the hydrogel in the absence of induction media. There is also a balanced expression of collagen relative to aggrecan production, a feature which was biased toward collagen production when cells were cultured with induction media. Lastly, metabolic profiles of each system show considerable overlap between both differentiation methods but subtle differences which potentially give rise to their resultant phenotype can be ascertained. The study highlights how material and chemical alterations in the cellular microenvironment have wide ranging effects on resultant tissue type.Enateri V. AlakpaVineetha JayawarnaKarl E. V. BurgessChristopher C. WestBruno PéaultRein V. UlijnMatthew J. DalbyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Enateri V. Alakpa Vineetha Jayawarna Karl E. V. Burgess Christopher C. West Bruno Péault Rein V. Ulijn Matthew J. Dalby Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels
description	Abstract Differentiation of stem cells to chondrocytes in vitro usually results in a heterogeneous phenotype. This is evident in the often detected over expression of type X collagen which, in hyaline cartilage structure is not characteristic of the mid-zone but of the deep-zone ossifying tissue. Methods to better match cartilage developed in vitro to characteristic in vivo features are therefore highly desirable in regenerative medicine. This study compares phenotype characteristics between pericytes, obtained from human adipose tissue, differentiated using diphenylalanine/serine (F2/S) peptide hydrogels with the more widely used chemical induced method for chondrogenesis. Significantly higher levels of type II collagen were noted when pericytes undergo chondrogenesis in the hydrogel in the absence of induction media. There is also a balanced expression of collagen relative to aggrecan production, a feature which was biased toward collagen production when cells were cultured with induction media. Lastly, metabolic profiles of each system show considerable overlap between both differentiation methods but subtle differences which potentially give rise to their resultant phenotype can be ascertained. The study highlights how material and chemical alterations in the cellular microenvironment have wide ranging effects on resultant tissue type.
format	article
author	Enateri V. Alakpa Vineetha Jayawarna Karl E. V. Burgess Christopher C. West Bruno Péault Rein V. Ulijn Matthew J. Dalby
author_facet	Enateri V. Alakpa Vineetha Jayawarna Karl E. V. Burgess Christopher C. West Bruno Péault Rein V. Ulijn Matthew J. Dalby
author_sort	Enateri V. Alakpa
title	Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels
title_short	Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels
title_full	Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels
title_fullStr	Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels
title_full_unstemmed	Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels
title_sort	improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/95134f7e2eff4a20a241be0b0bcaf930
work_keys_str_mv	AT enaterivalakpa improvingcartilagephenotypefromdifferentiatedpericytesintunablepeptidehydrogels AT vineethajayawarna improvingcartilagephenotypefromdifferentiatedpericytesintunablepeptidehydrogels AT karlevburgess improvingcartilagephenotypefromdifferentiatedpericytesintunablepeptidehydrogels AT christophercwest improvingcartilagephenotypefromdifferentiatedpericytesintunablepeptidehydrogels AT brunopeault improvingcartilagephenotypefromdifferentiatedpericytesintunablepeptidehydrogels AT reinvulijn improvingcartilagephenotypefromdifferentiatedpericytesintunablepeptidehydrogels AT matthewjdalby improvingcartilagephenotypefromdifferentiatedpericytesintunablepeptidehydrogels
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Improving cartilage phenotype from differentiated pericytes in tunable peptide hydrogels

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