Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function

Abstract Bushfires are increasing in frequency and severity worldwide. Bushfire smoke contains organic/inorganic compounds including aldehydes and acrolein. We described suppressive effects of tobacco smoke on the phagocytic capacity of airway macrophages, linked to secondary necrosis of uncleared a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rhys Hamon, Hai B. Tran, Eugene Roscioli, Miranda Ween, Hubertus Jersmann, Sandra Hodge
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/951fb943ffdc4a01ae08074cd1554b4b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:951fb943ffdc4a01ae08074cd1554b4b
record_format	dspace
spelling	oai:doaj.org-article:951fb943ffdc4a01ae08074cd1554b4b2021-12-02T15:09:07ZBushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function10.1038/s41598-018-31459-62045-2322https://doaj.org/article/951fb943ffdc4a01ae08074cd1554b4b2018-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-31459-6https://doaj.org/toc/2045-2322Abstract Bushfires are increasing in frequency and severity worldwide. Bushfire smoke contains organic/inorganic compounds including aldehydes and acrolein. We described suppressive effects of tobacco smoke on the phagocytic capacity of airway macrophages, linked to secondary necrosis of uncleared apoptotic epithelial cells, persistence of non-typeable H. influenzae (NTHi), and inflammation. We hypothesised that bushfire smoke extract (BFSE) would similarly impair macrophage function. THP-1 or monocyte-derived macrophages (MDM) were exposed to 1–10% BFSE prepared from foliage of 5 common Australian native plants (genus Acacia or Eucalyptus), or 10% cigarette smoke extract (CSE). Phagocytic recognition receptors were measured by flow cytometry; pro-inflammatory cytokines and caspase 1 by immunofluorescence or cytometric bead array; viability by LDH assay; and capsase-3/PARP by western blot. BFSE significantly decreased phagocytosis of apoptotic cells or NTHi by both THP-1 macrophages and MDM vs air control, consistent with the effects of CSE. BFSE significantly decreased MDM expression of CD36, CD44, SR-A1, CD206 and TLR-2 and increased active IL-1β, caspase-1 and secreted IL-8. BFSE dose-dependently decreased THP-1 macrophage viability (5-fold increase in LDH at 10%) and significantly increased active caspase-3. BFSE impairs macrophage function to a similar extent as CSE, highlighting the need for further research, especially in patients with pre-existing lung disease.Rhys HamonHai B. TranEugene RoscioliMiranda WeenHubertus JersmannSandra HodgeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rhys Hamon Hai B. Tran Eugene Roscioli Miranda Ween Hubertus Jersmann Sandra Hodge Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function
description	Abstract Bushfires are increasing in frequency and severity worldwide. Bushfire smoke contains organic/inorganic compounds including aldehydes and acrolein. We described suppressive effects of tobacco smoke on the phagocytic capacity of airway macrophages, linked to secondary necrosis of uncleared apoptotic epithelial cells, persistence of non-typeable H. influenzae (NTHi), and inflammation. We hypothesised that bushfire smoke extract (BFSE) would similarly impair macrophage function. THP-1 or monocyte-derived macrophages (MDM) were exposed to 1–10% BFSE prepared from foliage of 5 common Australian native plants (genus Acacia or Eucalyptus), or 10% cigarette smoke extract (CSE). Phagocytic recognition receptors were measured by flow cytometry; pro-inflammatory cytokines and caspase 1 by immunofluorescence or cytometric bead array; viability by LDH assay; and capsase-3/PARP by western blot. BFSE significantly decreased phagocytosis of apoptotic cells or NTHi by both THP-1 macrophages and MDM vs air control, consistent with the effects of CSE. BFSE significantly decreased MDM expression of CD36, CD44, SR-A1, CD206 and TLR-2 and increased active IL-1β, caspase-1 and secreted IL-8. BFSE dose-dependently decreased THP-1 macrophage viability (5-fold increase in LDH at 10%) and significantly increased active caspase-3. BFSE impairs macrophage function to a similar extent as CSE, highlighting the need for further research, especially in patients with pre-existing lung disease.
format	article
author	Rhys Hamon Hai B. Tran Eugene Roscioli Miranda Ween Hubertus Jersmann Sandra Hodge
author_facet	Rhys Hamon Hai B. Tran Eugene Roscioli Miranda Ween Hubertus Jersmann Sandra Hodge
author_sort	Rhys Hamon
title	Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function
title_short	Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function
title_full	Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function
title_fullStr	Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function
title_full_unstemmed	Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function
title_sort	bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/951fb943ffdc4a01ae08074cd1554b4b
work_keys_str_mv	AT rhyshamon bushfiresmokeisproinflammatoryandsuppressesmacrophagephagocyticfunction AT haibtran bushfiresmokeisproinflammatoryandsuppressesmacrophagephagocyticfunction AT eugeneroscioli bushfiresmokeisproinflammatoryandsuppressesmacrophagephagocyticfunction AT mirandaween bushfiresmokeisproinflammatoryandsuppressesmacrophagephagocyticfunction AT hubertusjersmann bushfiresmokeisproinflammatoryandsuppressesmacrophagephagocyticfunction AT sandrahodge bushfiresmokeisproinflammatoryandsuppressesmacrophagephagocyticfunction
_version_	1718387889676484608

Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function

Ejemplares similares