Identification of candidate serum proteins for classifying well-differentiated small intestinal neuroendocrine tumors.

<h4>Background</h4>Patients with well-differentiated small intestine neuroendocrine tumors (WD-SI-NETs) are most often diagnosed at a metastatic stage of disease, which reduces possibilities for a curative treatment. Thus new approaches for earlier detection and improved monitoring of th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Spyros Darmanis, Tao Cui, Kimi Drobin, Su-Chen Li, Kjell Öberg, Peter Nilsson, Jochen M Schwenk, Valeria Giandomenico
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95213b45ed6941ebb03e9d951ad2a751
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:95213b45ed6941ebb03e9d951ad2a751
record_format	dspace
spelling	oai:doaj.org-article:95213b45ed6941ebb03e9d951ad2a7512021-11-18T08:44:46ZIdentification of candidate serum proteins for classifying well-differentiated small intestinal neuroendocrine tumors.1932-620310.1371/journal.pone.0081712https://doaj.org/article/95213b45ed6941ebb03e9d951ad2a7512013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24282616/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Patients with well-differentiated small intestine neuroendocrine tumors (WD-SI-NETs) are most often diagnosed at a metastatic stage of disease, which reduces possibilities for a curative treatment. Thus new approaches for earlier detection and improved monitoring of the disease are required.<h4>Materials and methods</h4>Suspension bead arrays targeting 124 unique proteins with antibodies from the Human Protein Atlas were used to profile biotinylated serum samples. Discoveries from a cohort of 77 individuals were followed up in a cohort of 132 individuals both including healthy controls as well as patients with untreated primary WD-SI-NETs, lymph node metastases and liver metastases.<h4>Results</h4>A set of 20 antibodies suggested promising proteins for further verification based on technically verified statistical significance. Proceeding, we assessed the classification performance in an independent cohort of patient serum, achieving, classification accuracy of up to 85% with different subsets of antibodies in respective pairwise group comparisons. The protein profiles of nine targets, namely IGFBP2, IGF1, SHKBP1, ETS1, IL1α, STX2, MAML3, EGR3 and XIAP were verified as significant contributors to tumor classification.<h4>Conclusions</h4>We propose new potential protein biomarker candidates for classifying WD-SI-NETs at different stage of disease. Further evaluation of these proteins in larger sample sets and with alternative approaches is needed in order to further improve our understanding of their functional relation to WD-SI-NETs and their eventual use in diagnostics.Spyros DarmanisTao CuiKimi DrobinSu-Chen LiKjell ÖbergPeter NilssonJochen M SchwenkValeria GiandomenicoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e81712 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Spyros Darmanis Tao Cui Kimi Drobin Su-Chen Li Kjell Öberg Peter Nilsson Jochen M Schwenk Valeria Giandomenico Identification of candidate serum proteins for classifying well-differentiated small intestinal neuroendocrine tumors.
description	<h4>Background</h4>Patients with well-differentiated small intestine neuroendocrine tumors (WD-SI-NETs) are most often diagnosed at a metastatic stage of disease, which reduces possibilities for a curative treatment. Thus new approaches for earlier detection and improved monitoring of the disease are required.<h4>Materials and methods</h4>Suspension bead arrays targeting 124 unique proteins with antibodies from the Human Protein Atlas were used to profile biotinylated serum samples. Discoveries from a cohort of 77 individuals were followed up in a cohort of 132 individuals both including healthy controls as well as patients with untreated primary WD-SI-NETs, lymph node metastases and liver metastases.<h4>Results</h4>A set of 20 antibodies suggested promising proteins for further verification based on technically verified statistical significance. Proceeding, we assessed the classification performance in an independent cohort of patient serum, achieving, classification accuracy of up to 85% with different subsets of antibodies in respective pairwise group comparisons. The protein profiles of nine targets, namely IGFBP2, IGF1, SHKBP1, ETS1, IL1α, STX2, MAML3, EGR3 and XIAP were verified as significant contributors to tumor classification.<h4>Conclusions</h4>We propose new potential protein biomarker candidates for classifying WD-SI-NETs at different stage of disease. Further evaluation of these proteins in larger sample sets and with alternative approaches is needed in order to further improve our understanding of their functional relation to WD-SI-NETs and their eventual use in diagnostics.
format	article
author	Spyros Darmanis Tao Cui Kimi Drobin Su-Chen Li Kjell Öberg Peter Nilsson Jochen M Schwenk Valeria Giandomenico
author_facet	Spyros Darmanis Tao Cui Kimi Drobin Su-Chen Li Kjell Öberg Peter Nilsson Jochen M Schwenk Valeria Giandomenico
author_sort	Spyros Darmanis
title	Identification of candidate serum proteins for classifying well-differentiated small intestinal neuroendocrine tumors.
title_short	Identification of candidate serum proteins for classifying well-differentiated small intestinal neuroendocrine tumors.
title_full	Identification of candidate serum proteins for classifying well-differentiated small intestinal neuroendocrine tumors.
title_fullStr	Identification of candidate serum proteins for classifying well-differentiated small intestinal neuroendocrine tumors.
title_full_unstemmed	Identification of candidate serum proteins for classifying well-differentiated small intestinal neuroendocrine tumors.
title_sort	identification of candidate serum proteins for classifying well-differentiated small intestinal neuroendocrine tumors.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/95213b45ed6941ebb03e9d951ad2a751
work_keys_str_mv	AT spyrosdarmanis identificationofcandidateserumproteinsforclassifyingwelldifferentiatedsmallintestinalneuroendocrinetumors AT taocui identificationofcandidateserumproteinsforclassifyingwelldifferentiatedsmallintestinalneuroendocrinetumors AT kimidrobin identificationofcandidateserumproteinsforclassifyingwelldifferentiatedsmallintestinalneuroendocrinetumors AT suchenli identificationofcandidateserumproteinsforclassifyingwelldifferentiatedsmallintestinalneuroendocrinetumors AT kjelloberg identificationofcandidateserumproteinsforclassifyingwelldifferentiatedsmallintestinalneuroendocrinetumors AT peternilsson identificationofcandidateserumproteinsforclassifyingwelldifferentiatedsmallintestinalneuroendocrinetumors AT jochenmschwenk identificationofcandidateserumproteinsforclassifyingwelldifferentiatedsmallintestinalneuroendocrinetumors AT valeriagiandomenico identificationofcandidateserumproteinsforclassifyingwelldifferentiatedsmallintestinalneuroendocrinetumors
_version_	1718421397185757184

Identification of candidate serum proteins for classifying well-differentiated small intestinal neuroendocrine tumors.

Ejemplares similares