Evaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, Phaedon brassicae (Coleoptera: Chrysomelidae).

The brassica leaf beetle Phaedon brassicae is a notorious defoliator of cruciferous vegetables. However, few molecular studies of this pest have been conducted due to limited sequence data. Recently, RNA sequencing has offered a powerful platform to generate numerous transcriptomic data, which requi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Long Ma, Ting Jiang, Xiangya Liu, Haijun Xiao, Yingchuan Peng, Wanna Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95231848d66a4e86924507f62e164bcf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:95231848d66a4e86924507f62e164bcf
record_format	dspace
spelling	oai:doaj.org-article:95231848d66a4e86924507f62e164bcf2021-12-02T20:03:59ZEvaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, Phaedon brassicae (Coleoptera: Chrysomelidae).1932-620310.1371/journal.pone.0251920https://doaj.org/article/95231848d66a4e86924507f62e164bcf2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251920https://doaj.org/toc/1932-6203The brassica leaf beetle Phaedon brassicae is a notorious defoliator of cruciferous vegetables. However, few molecular studies of this pest have been conducted due to limited sequence data. Recently, RNA sequencing has offered a powerful platform to generate numerous transcriptomic data, which require RT-qPCR to validate target gene expression. The selection of reliable reference genes to normalize RT-qPCR data is a prerequisite for gene expression analysis. In the present study, the expression stabilities of eight candidate reference genes under biotic conditions (development stages and various tissues) and abiotic perturbations (thermal stress and pesticide exposure) were evaluated using four different statistical algorithms. The optimal suites of reference genes were recommended for the respective experimental conditions. For tissue expression analysis, RPL32 and EF-1α were recommended as the suitable reference genes. RPL19 and TBP were the optimal reference genes across different developmental stages. RPL32 and TBP were identified as the most suitable references for thermal stress. Furthermore, RPL32 and RPL19 were ranked as the best references for insecticide exposure. This work provides a systematic exploration of the optimal reference genes for the respective experimental conditions, and our findings would facilitate molecular studies of P. brassicae.Long MaTing JiangXiangya LiuHaijun XiaoYingchuan PengWanna ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0251920 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Long Ma Ting Jiang Xiangya Liu Haijun Xiao Yingchuan Peng Wanna Zhang Evaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, Phaedon brassicae (Coleoptera: Chrysomelidae).
description	The brassica leaf beetle Phaedon brassicae is a notorious defoliator of cruciferous vegetables. However, few molecular studies of this pest have been conducted due to limited sequence data. Recently, RNA sequencing has offered a powerful platform to generate numerous transcriptomic data, which require RT-qPCR to validate target gene expression. The selection of reliable reference genes to normalize RT-qPCR data is a prerequisite for gene expression analysis. In the present study, the expression stabilities of eight candidate reference genes under biotic conditions (development stages and various tissues) and abiotic perturbations (thermal stress and pesticide exposure) were evaluated using four different statistical algorithms. The optimal suites of reference genes were recommended for the respective experimental conditions. For tissue expression analysis, RPL32 and EF-1α were recommended as the suitable reference genes. RPL19 and TBP were the optimal reference genes across different developmental stages. RPL32 and TBP were identified as the most suitable references for thermal stress. Furthermore, RPL32 and RPL19 were ranked as the best references for insecticide exposure. This work provides a systematic exploration of the optimal reference genes for the respective experimental conditions, and our findings would facilitate molecular studies of P. brassicae.
format	article
author	Long Ma Ting Jiang Xiangya Liu Haijun Xiao Yingchuan Peng Wanna Zhang
author_facet	Long Ma Ting Jiang Xiangya Liu Haijun Xiao Yingchuan Peng Wanna Zhang
author_sort	Long Ma
title	Evaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, Phaedon brassicae (Coleoptera: Chrysomelidae).
title_short	Evaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, Phaedon brassicae (Coleoptera: Chrysomelidae).
title_full	Evaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, Phaedon brassicae (Coleoptera: Chrysomelidae).
title_fullStr	Evaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, Phaedon brassicae (Coleoptera: Chrysomelidae).
title_full_unstemmed	Evaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, Phaedon brassicae (Coleoptera: Chrysomelidae).
title_sort	evaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, phaedon brassicae (coleoptera: chrysomelidae).
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/95231848d66a4e86924507f62e164bcf
work_keys_str_mv	AT longma evaluationofcandidatereferencegenesforgeneexpressionanalysisinthebrassicaleafbeetlephaedonbrassicaecoleopterachrysomelidae AT tingjiang evaluationofcandidatereferencegenesforgeneexpressionanalysisinthebrassicaleafbeetlephaedonbrassicaecoleopterachrysomelidae AT xiangyaliu evaluationofcandidatereferencegenesforgeneexpressionanalysisinthebrassicaleafbeetlephaedonbrassicaecoleopterachrysomelidae AT haijunxiao evaluationofcandidatereferencegenesforgeneexpressionanalysisinthebrassicaleafbeetlephaedonbrassicaecoleopterachrysomelidae AT yingchuanpeng evaluationofcandidatereferencegenesforgeneexpressionanalysisinthebrassicaleafbeetlephaedonbrassicaecoleopterachrysomelidae AT wannazhang evaluationofcandidatereferencegenesforgeneexpressionanalysisinthebrassicaleafbeetlephaedonbrassicaecoleopterachrysomelidae
_version_	1718375617960869888

Evaluation of candidate reference genes for gene expression analysis in the brassica leaf beetle, Phaedon brassicae (Coleoptera: Chrysomelidae).

Ejemplares similares