Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival

Abstract Salmonella have developed a sophisticated machinery to evade immune clearance and promote survival in the infected cells. Previous studies were mostly focused on either bacteria itself or host cells, the interaction mechanism of host-pathogen awaits further exploration. In the present study...

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Autores principales:	Hongwei Gu, Chihao Zhao, Tianfu Zhang, Hongwei Liang, Xiao-Ming Wang, Yi Pan, Xi Chen, Quan Zhao, Donghai Li, Fenyong Liu, Chen-Yu Zhang, Ke Zen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/952433644de74413885e7a51524769b0
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spelling	oai:doaj.org-article:952433644de74413885e7a51524769b02021-12-02T15:05:52ZSalmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival10.1038/s41598-017-02669-12045-2322https://doaj.org/article/952433644de74413885e7a51524769b02017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02669-1https://doaj.org/toc/2045-2322Abstract Salmonella have developed a sophisticated machinery to evade immune clearance and promote survival in the infected cells. Previous studies were mostly focused on either bacteria itself or host cells, the interaction mechanism of host-pathogen awaits further exploration. In the present study, we show that Salmonella can exploit mammalian cell non-classical microRNA processing machinery to further process bacterial small non-coding RNAs into microRNA-like fragments. Sal-1, one such fragment with the highest copy number in the infected cells, is derived from Salmonella 5′-leader of the ribosomal RNA transcript and has a ‘stem’ structure-containing precursor. Processing of Sal-1 precursors to mature Sal-1 is dependent on host cell Argonaute 2 (AGO2) but not Dicer. Functionally, depleting cellular Sal-1 strongly renders the Salmonella bacteria less resistant to the host defenses both in vitro and in vivo. In conclusion, we demonstrate a novel strategy for Salmonella evading the host immune clearance, in which Salmonella produce microRNA-like functional RNA fragments to establish a microenvironment facilitating bacterial survival.Hongwei GuChihao ZhaoTianfu ZhangHongwei LiangXiao-Ming WangYi PanXi ChenQuan ZhaoDonghai LiFenyong LiuChen-Yu ZhangKe ZenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hongwei Gu Chihao Zhao Tianfu Zhang Hongwei Liang Xiao-Ming Wang Yi Pan Xi Chen Quan Zhao Donghai Li Fenyong Liu Chen-Yu Zhang Ke Zen Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival
description	Abstract Salmonella have developed a sophisticated machinery to evade immune clearance and promote survival in the infected cells. Previous studies were mostly focused on either bacteria itself or host cells, the interaction mechanism of host-pathogen awaits further exploration. In the present study, we show that Salmonella can exploit mammalian cell non-classical microRNA processing machinery to further process bacterial small non-coding RNAs into microRNA-like fragments. Sal-1, one such fragment with the highest copy number in the infected cells, is derived from Salmonella 5′-leader of the ribosomal RNA transcript and has a ‘stem’ structure-containing precursor. Processing of Sal-1 precursors to mature Sal-1 is dependent on host cell Argonaute 2 (AGO2) but not Dicer. Functionally, depleting cellular Sal-1 strongly renders the Salmonella bacteria less resistant to the host defenses both in vitro and in vivo. In conclusion, we demonstrate a novel strategy for Salmonella evading the host immune clearance, in which Salmonella produce microRNA-like functional RNA fragments to establish a microenvironment facilitating bacterial survival.
format	article
author	Hongwei Gu Chihao Zhao Tianfu Zhang Hongwei Liang Xiao-Ming Wang Yi Pan Xi Chen Quan Zhao Donghai Li Fenyong Liu Chen-Yu Zhang Ke Zen
author_facet	Hongwei Gu Chihao Zhao Tianfu Zhang Hongwei Liang Xiao-Ming Wang Yi Pan Xi Chen Quan Zhao Donghai Li Fenyong Liu Chen-Yu Zhang Ke Zen
author_sort	Hongwei Gu
title	Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival
title_short	Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival
title_full	Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival
title_fullStr	Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival
title_full_unstemmed	Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival
title_sort	salmonella produce microrna-like rna fragment sal-1 in the infected cells to facilitate intracellular survival
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/952433644de74413885e7a51524769b0
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Salmonella produce microRNA-like RNA fragment Sal-1 in the infected cells to facilitate intracellular survival

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