Relationship between circulating and tissue microRNAs in a murine model of breast cancer.

MiRNAs are key regulators of tumorigenesis that are aberrantly expressed in the circulation and tissue of patients with cancer. The aim of this study was to determine whether miRNA dysregulation in the circulation reflected similar changes in tumour tissue. Athymic nude mice (n = 20) received either...

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Autores principales:	Peadar S Waters, Ailbhe M McDermott, Deirdre Wall, Helen M Heneghan, Nicola Miller, John Newell, Michael J Kerin, Roisin M Dwyer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/952c92e5ad004dd6869b0e7898b487b5
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spelling	oai:doaj.org-article:952c92e5ad004dd6869b0e7898b487b52021-11-18T08:06:51ZRelationship between circulating and tissue microRNAs in a murine model of breast cancer.1932-620310.1371/journal.pone.0050459https://doaj.org/article/952c92e5ad004dd6869b0e7898b487b52012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226290/?tool=EBIhttps://doaj.org/toc/1932-6203MiRNAs are key regulators of tumorigenesis that are aberrantly expressed in the circulation and tissue of patients with cancer. The aim of this study was to determine whether miRNA dysregulation in the circulation reflected similar changes in tumour tissue. Athymic nude mice (n = 20) received either a mammary fat pad (n = 8, MFP), or subcutaneous (n = 7, SC) injection of MDA-MB-231 cells. Controls received no tumour cells (n = 5). Tumour volume was monitored weekly and blood sampling performed at weeks 1, 3 and 6 following tumour induction (total n = 60). Animals were sacrificed at week 6 and tumour tissue (n = 15), lungs (n = 20) and enlarged lymph nodes (n = 3) harvested. MicroRNAs were extracted from all samples (n = 98) and relative expression quantified using RQ-PCR. MiR-221 expression was significantly increased in tumour compared to healthy tissue (p<0.001). MiR-10b expression was significantly higher in MFP compared to SC tumours (p<0.05), with the highest levels detected in diseased lymph nodes (p<0.05). MiR-10b was undetectable in the circulation, with no significant change in circulating miR-221 expression detected during disease progression. MiR-195 and miR-497 were significantly decreased in tumour tissue (p<0.05), and also in the circulation of animals 3 weeks following tumour induction (p<0.05). At both tissue and circulating level, a positive correlation was observed between miR-497 and miR-195 (r = 0.61, p<0.001; r = 0.41, p<0.01 respectively). This study highlights the distinct roles of miRNAs in circulation and tissue. It also implicates miRNAs in disease dissemination and progression, which may be important in systemic therapy and biomarker development.Peadar S WatersAilbhe M McDermottDeirdre WallHelen M HeneghanNicola MillerJohn NewellMichael J KerinRoisin M DwyerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50459 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peadar S Waters Ailbhe M McDermott Deirdre Wall Helen M Heneghan Nicola Miller John Newell Michael J Kerin Roisin M Dwyer Relationship between circulating and tissue microRNAs in a murine model of breast cancer.
description	MiRNAs are key regulators of tumorigenesis that are aberrantly expressed in the circulation and tissue of patients with cancer. The aim of this study was to determine whether miRNA dysregulation in the circulation reflected similar changes in tumour tissue. Athymic nude mice (n = 20) received either a mammary fat pad (n = 8, MFP), or subcutaneous (n = 7, SC) injection of MDA-MB-231 cells. Controls received no tumour cells (n = 5). Tumour volume was monitored weekly and blood sampling performed at weeks 1, 3 and 6 following tumour induction (total n = 60). Animals were sacrificed at week 6 and tumour tissue (n = 15), lungs (n = 20) and enlarged lymph nodes (n = 3) harvested. MicroRNAs were extracted from all samples (n = 98) and relative expression quantified using RQ-PCR. MiR-221 expression was significantly increased in tumour compared to healthy tissue (p<0.001). MiR-10b expression was significantly higher in MFP compared to SC tumours (p<0.05), with the highest levels detected in diseased lymph nodes (p<0.05). MiR-10b was undetectable in the circulation, with no significant change in circulating miR-221 expression detected during disease progression. MiR-195 and miR-497 were significantly decreased in tumour tissue (p<0.05), and also in the circulation of animals 3 weeks following tumour induction (p<0.05). At both tissue and circulating level, a positive correlation was observed between miR-497 and miR-195 (r = 0.61, p<0.001; r = 0.41, p<0.01 respectively). This study highlights the distinct roles of miRNAs in circulation and tissue. It also implicates miRNAs in disease dissemination and progression, which may be important in systemic therapy and biomarker development.
format	article
author	Peadar S Waters Ailbhe M McDermott Deirdre Wall Helen M Heneghan Nicola Miller John Newell Michael J Kerin Roisin M Dwyer
author_facet	Peadar S Waters Ailbhe M McDermott Deirdre Wall Helen M Heneghan Nicola Miller John Newell Michael J Kerin Roisin M Dwyer
author_sort	Peadar S Waters
title	Relationship between circulating and tissue microRNAs in a murine model of breast cancer.
title_short	Relationship between circulating and tissue microRNAs in a murine model of breast cancer.
title_full	Relationship between circulating and tissue microRNAs in a murine model of breast cancer.
title_fullStr	Relationship between circulating and tissue microRNAs in a murine model of breast cancer.
title_full_unstemmed	Relationship between circulating and tissue microRNAs in a murine model of breast cancer.
title_sort	relationship between circulating and tissue micrornas in a murine model of breast cancer.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/952c92e5ad004dd6869b0e7898b487b5
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Relationship between circulating and tissue microRNAs in a murine model of breast cancer.

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