Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model

Ning Zhu, Xue Yan, Hongli Li, Huiqin Wang Department 1 of Cardiology, Baoding First Center Hospital, Baoding 071002, Hebei, People’s Republic of ChinaCorrespondence: Ning ZhuDepartment 1 of Cardiology, Baoding First Center Hospital, 320 Changchengbeidajie, Baoding 071002, Hebei, People&...

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Autores principales:	Zhu N, Yan X, Li H, Wang H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	pgc-1 alpha ros oxidative stress diabetes mellitus with myocardial infarction Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/953b4a5c5b0445b49a86c06fe37ca2c7
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spelling	oai:doaj.org-article:953b4a5c5b0445b49a86c06fe37ca2c72021-12-02T12:31:39ZClinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model1178-7007https://doaj.org/article/953b4a5c5b0445b49a86c06fe37ca2c72020-10-01T00:00:00Zhttps://www.dovepress.com/clinical-significance-of-serum-pgc-1-alpha-levels-in-diabetes-mellitus-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Ning Zhu, Xue Yan, Hongli Li, Huiqin Wang Department 1 of Cardiology, Baoding First Center Hospital, Baoding 071002, Hebei, People’s Republic of ChinaCorrespondence: Ning ZhuDepartment 1 of Cardiology, Baoding First Center Hospital, 320 Changchengbeidajie, Baoding 071002, Hebei, People’s Republic of ChinaTel +86-312-5096409Email zhuning_my@21cn.comBackground: In this study, we explored the clinical significance of serum peroxisome proliferator-activated receptor gamma co-activator 1 (PGC-1) alpha levels in diabetes mellitus with myocardial infarction (DMMI) patients and investigated the possible mechanism.Materials and Methods: Serum samples were obtained from patients with DMMI or normal volunteer in Baoding First Center Hospital. C57BL/6 mice were induced by a single intraperitoneal (i.p.) injection of 100 mg/kg STZ (streptozocin) for in vivo model. Human myocardial cell lines H9C2 cells were induced with high glucose medium (33 mmol/L glucose) for in vitro model. Western blot was used to analyze the protein expressions in this study.Results: Serum PGC-1 alpha levels were down-regulated in patients with DMMI. There was negative correlation between serum PGC-1 alpha levels and glycated hemoglobin, blood glucose or glucagon in DMMI patients. Recombination of PGC-1 alpha protein decreased the levels of glycated hemoglobin, blood glucose and glucagon, and inhibited oxidative stress and myocardial damage in mice of DMMI. Over-expression of PGC-1 alpha reduced reactive oxygen species (ROS)-oxidative stress, while down-regulation of PGC-1 alpha promoted ROS-oxidative stress via regulation of hemeoxygenase− 1 (HO-1) expression in in vitro model of DMMI. The inhibition of HO-1 expression attenuated the anti-oxidation effects of PGC-1 alpha in vitro.Conclusion: PGC-1 alpha attenuated ROS-oxidative stress in diabetic cardiomyopathy model, and PGC-1 alpha served as a potential intervention to alleviate DMMI in clinical applications.Keywords: PGC-1 alpha, ROS, oxidative stress, diabetes mellitus with myocardial infarctionZhu NYan XLi HWang HDove Medical Pressarticlepgc-1 alpharosoxidative stressdiabetes mellitus with myocardial infarctionSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 4041-4049 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	pgc-1 alpha ros oxidative stress diabetes mellitus with myocardial infarction Specialties of internal medicine RC581-951
spellingShingle	pgc-1 alpha ros oxidative stress diabetes mellitus with myocardial infarction Specialties of internal medicine RC581-951 Zhu N Yan X Li H Wang H Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model
description	Ning Zhu, Xue Yan, Hongli Li, Huiqin Wang Department 1 of Cardiology, Baoding First Center Hospital, Baoding 071002, Hebei, People’s Republic of ChinaCorrespondence: Ning ZhuDepartment 1 of Cardiology, Baoding First Center Hospital, 320 Changchengbeidajie, Baoding 071002, Hebei, People’s Republic of ChinaTel +86-312-5096409Email zhuning_my@21cn.comBackground: In this study, we explored the clinical significance of serum peroxisome proliferator-activated receptor gamma co-activator 1 (PGC-1) alpha levels in diabetes mellitus with myocardial infarction (DMMI) patients and investigated the possible mechanism.Materials and Methods: Serum samples were obtained from patients with DMMI or normal volunteer in Baoding First Center Hospital. C57BL/6 mice were induced by a single intraperitoneal (i.p.) injection of 100 mg/kg STZ (streptozocin) for in vivo model. Human myocardial cell lines H9C2 cells were induced with high glucose medium (33 mmol/L glucose) for in vitro model. Western blot was used to analyze the protein expressions in this study.Results: Serum PGC-1 alpha levels were down-regulated in patients with DMMI. There was negative correlation between serum PGC-1 alpha levels and glycated hemoglobin, blood glucose or glucagon in DMMI patients. Recombination of PGC-1 alpha protein decreased the levels of glycated hemoglobin, blood glucose and glucagon, and inhibited oxidative stress and myocardial damage in mice of DMMI. Over-expression of PGC-1 alpha reduced reactive oxygen species (ROS)-oxidative stress, while down-regulation of PGC-1 alpha promoted ROS-oxidative stress via regulation of hemeoxygenase− 1 (HO-1) expression in in vitro model of DMMI. The inhibition of HO-1 expression attenuated the anti-oxidation effects of PGC-1 alpha in vitro.Conclusion: PGC-1 alpha attenuated ROS-oxidative stress in diabetic cardiomyopathy model, and PGC-1 alpha served as a potential intervention to alleviate DMMI in clinical applications.Keywords: PGC-1 alpha, ROS, oxidative stress, diabetes mellitus with myocardial infarction
format	article
author	Zhu N Yan X Li H Wang H
author_facet	Zhu N Yan X Li H Wang H
author_sort	Zhu N
title	Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model
title_short	Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model
title_full	Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model
title_fullStr	Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model
title_full_unstemmed	Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model
title_sort	clinical significance of serum pgc-1 alpha levels in diabetes mellitus with myocardial infarction patients and reduced ros-oxidative stress in diabetes mellitus with myocardial infarction model
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/953b4a5c5b0445b49a86c06fe37ca2c7
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Clinical Significance of Serum PGC-1 Alpha Levels in Diabetes Mellitus with Myocardial Infarction Patients and Reduced ROS-Oxidative Stress in Diabetes Mellitus with Myocardial Infarction Model

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