Cloning, Expression, and Tobacco Overexpression Analyses of a <i>PISTILLATA</i>/<i>GLOBOSA</i>-like (<i>OfGLO1</i>) Gene from <i>Osmanthus fragrans</i>
<i>GLOBOSA</i> (<i>GLO</i>), a B-class MADS-box gene, is involved in floral organ determination but has rarely been studied in <i>Osmanthus fragrans</i>, which is a very popular ornamental tree species in China. Here, the full-length cDNA of a homologous <i>...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/953c7177a7904c129903b3eb9fc84cc8 |
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| Sumario: | <i>GLOBOSA</i> (<i>GLO</i>), a B-class MADS-box gene, is involved in floral organ determination but has rarely been studied in <i>Osmanthus fragrans</i>, which is a very popular ornamental tree species in China. Here, the full-length cDNA of a homologous <i>GLO1</i> gene (named <i>OfGLO1</i>) was cloned from a flower bud of <i>O. fragrans</i> using the RACE technique. The <i>OfGLO1</i> has a 645 bp open reading frame, encoding 214 amino acids. Similar to other PI/GLO proteins, OfGLO1 has two conserved domains, MADS MEF2-like and K-box, and a 16-amino-acid PI motif in the C terminal region. Our phylogeny analysis classified OfGLO1 as a PI-type member of the B-class MADS-box gene family. The qRT-PCR assay showed that the expression of <i>OfGLO1</i> in <i>O. fragrans</i> was continuously upregulated from the tight bud stage to the full flowering stage but barely expressed in the pistils, sepals, and non-floral organs, such as root, leaf, and stem. The genetic effect of <i>OfGLO1</i> was assayed by ectopic expression in tobacco plants. Compared with the wild-type, <i>OfGLO1</i> transformants showed reduced plant size, earlier flowering, shorter stamens, and lower seed setting rates. Furthermore, some stamens were changed into petal-like structures. These findings indicate that <i>OfGLO1</i> plays an important role in the regulation of flower development. This study improved our understanding of class B gene function in woody plants. |
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