Cloning, Expression, and Tobacco Overexpression Analyses of a PISTILLATA/GLOBOSA-like (OfGLO1) Gene from Osmanthus fragrans

GLOBOSA (GLO), a B-class MADS-box gene, is involved in floral organ determination but has rarely been studied in Osmanthus fragrans, which is a very popular ornamental tree species in China. Here, the full-length cDNA of a homologous ...

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Autores principales:	Zhanghui Zeng, Si Chen, Mingrui Xu, Min Wang, Zhehao Chen, Lilin Wang, Jiliang Pang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>OfGLO1</i> <i>Osmanthus fragrans</i> B-class MADS-box ectopic expression flower organ development Genetics QH426-470
Acceso en línea:	https://doaj.org/article/953c7177a7904c129903b3eb9fc84cc8
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spelling	oai:doaj.org-article:953c7177a7904c129903b3eb9fc84cc82021-11-25T17:41:30ZCloning, Expression, and Tobacco Overexpression Analyses of a <i>PISTILLATA</i>/<i>GLOBOSA</i>-like (<i>OfGLO1</i>) Gene from <i>Osmanthus fragrans</i>10.3390/genes121117482073-4425https://doaj.org/article/953c7177a7904c129903b3eb9fc84cc82021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1748https://doaj.org/toc/2073-4425<i>GLOBOSA</i> (<i>GLO</i>), a B-class MADS-box gene, is involved in floral organ determination but has rarely been studied in <i>Osmanthus fragrans</i>, which is a very popular ornamental tree species in China. Here, the full-length cDNA of a homologous <i>GLO1</i> gene (named <i>OfGLO1</i>) was cloned from a flower bud of <i>O. fragrans</i> using the RACE technique. The <i>OfGLO1</i> has a 645 bp open reading frame, encoding 214 amino acids. Similar to other PI/GLO proteins, OfGLO1 has two conserved domains, MADS MEF2-like and K-box, and a 16-amino-acid PI motif in the C terminal region. Our phylogeny analysis classified OfGLO1 as a PI-type member of the B-class MADS-box gene family. The qRT-PCR assay showed that the expression of <i>OfGLO1</i> in <i>O. fragrans</i> was continuously upregulated from the tight bud stage to the full flowering stage but barely expressed in the pistils, sepals, and non-floral organs, such as root, leaf, and stem. The genetic effect of <i>OfGLO1</i> was assayed by ectopic expression in tobacco plants. Compared with the wild-type, <i>OfGLO1</i> transformants showed reduced plant size, earlier flowering, shorter stamens, and lower seed setting rates. Furthermore, some stamens were changed into petal-like structures. These findings indicate that <i>OfGLO1</i> plays an important role in the regulation of flower development. This study improved our understanding of class B gene function in woody plants.Zhanghui ZengSi ChenMingrui XuMin WangZhehao ChenLilin WangJiliang PangMDPI AGarticle<i>OfGLO1</i><i>Osmanthus fragrans</i>B-class MADS-boxectopic expressionflower organ developmentGeneticsQH426-470ENGenes, Vol 12, Iss 1748, p 1748 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>OfGLO1</i> <i>Osmanthus fragrans</i> B-class MADS-box ectopic expression flower organ development Genetics QH426-470
spellingShingle	<i>OfGLO1</i> <i>Osmanthus fragrans</i> B-class MADS-box ectopic expression flower organ development Genetics QH426-470 Zhanghui Zeng Si Chen Mingrui Xu Min Wang Zhehao Chen Lilin Wang Jiliang Pang Cloning, Expression, and Tobacco Overexpression Analyses of a <i>PISTILLATA</i>/<i>GLOBOSA</i>-like (<i>OfGLO1</i>) Gene from <i>Osmanthus fragrans</i>
description	<i>GLOBOSA</i> (<i>GLO</i>), a B-class MADS-box gene, is involved in floral organ determination but has rarely been studied in <i>Osmanthus fragrans</i>, which is a very popular ornamental tree species in China. Here, the full-length cDNA of a homologous <i>GLO1</i> gene (named <i>OfGLO1</i>) was cloned from a flower bud of <i>O. fragrans</i> using the RACE technique. The <i>OfGLO1</i> has a 645 bp open reading frame, encoding 214 amino acids. Similar to other PI/GLO proteins, OfGLO1 has two conserved domains, MADS MEF2-like and K-box, and a 16-amino-acid PI motif in the C terminal region. Our phylogeny analysis classified OfGLO1 as a PI-type member of the B-class MADS-box gene family. The qRT-PCR assay showed that the expression of <i>OfGLO1</i> in <i>O. fragrans</i> was continuously upregulated from the tight bud stage to the full flowering stage but barely expressed in the pistils, sepals, and non-floral organs, such as root, leaf, and stem. The genetic effect of <i>OfGLO1</i> was assayed by ectopic expression in tobacco plants. Compared with the wild-type, <i>OfGLO1</i> transformants showed reduced plant size, earlier flowering, shorter stamens, and lower seed setting rates. Furthermore, some stamens were changed into petal-like structures. These findings indicate that <i>OfGLO1</i> plays an important role in the regulation of flower development. This study improved our understanding of class B gene function in woody plants.
format	article
author	Zhanghui Zeng Si Chen Mingrui Xu Min Wang Zhehao Chen Lilin Wang Jiliang Pang
author_facet	Zhanghui Zeng Si Chen Mingrui Xu Min Wang Zhehao Chen Lilin Wang Jiliang Pang
author_sort	Zhanghui Zeng
title	Cloning, Expression, and Tobacco Overexpression Analyses of a <i>PISTILLATA</i>/<i>GLOBOSA</i>-like (<i>OfGLO1</i>) Gene from <i>Osmanthus fragrans</i>
title_short	Cloning, Expression, and Tobacco Overexpression Analyses of a <i>PISTILLATA</i>/<i>GLOBOSA</i>-like (<i>OfGLO1</i>) Gene from <i>Osmanthus fragrans</i>
title_full	Cloning, Expression, and Tobacco Overexpression Analyses of a <i>PISTILLATA</i>/<i>GLOBOSA</i>-like (<i>OfGLO1</i>) Gene from <i>Osmanthus fragrans</i>
title_fullStr	Cloning, Expression, and Tobacco Overexpression Analyses of a <i>PISTILLATA</i>/<i>GLOBOSA</i>-like (<i>OfGLO1</i>) Gene from <i>Osmanthus fragrans</i>
title_full_unstemmed	Cloning, Expression, and Tobacco Overexpression Analyses of a <i>PISTILLATA</i>/<i>GLOBOSA</i>-like (<i>OfGLO1</i>) Gene from <i>Osmanthus fragrans</i>
title_sort	cloning, expression, and tobacco overexpression analyses of a <i>pistillata</i>/<i>globosa</i>-like (<i>ofglo1</i>) gene from <i>osmanthus fragrans</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/953c7177a7904c129903b3eb9fc84cc8
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Cloning, Expression, and Tobacco Overexpression Analyses of a <i>PISTILLATA</i>/<i>GLOBOSA</i>-like (<i>OfGLO1</i>) Gene from <i>Osmanthus fragrans</i>

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