Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture

Abstract Kluyveromyces marxianus is a yeast that could be identified from kefir and can use a broad range of substrates, such as glucose and lactate, as carbon sources. The lactate produced in kefir culture can be a substrate for K. marxianus. However, the complexity of the kefir microbiota makes th...

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Autores principales:	Shou-Chen Lo, Chia-Yin Yang, Dony Chacko Mathew, Chieh-Chen Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95436ba6c6fe4f359e3699ef64a6388e
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spelling	oai:doaj.org-article:95436ba6c6fe4f359e3699ef64a6388e2021-12-02T15:33:00ZGrowth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture10.1038/s41598-021-94101-y2045-2322https://doaj.org/article/95436ba6c6fe4f359e3699ef64a6388e2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94101-yhttps://doaj.org/toc/2045-2322Abstract Kluyveromyces marxianus is a yeast that could be identified from kefir and can use a broad range of substrates, such as glucose and lactate, as carbon sources. The lactate produced in kefir culture can be a substrate for K. marxianus. However, the complexity of the kefir microbiota makes the traits of K. marxianus difficult to study. In this research, we focused on K. marxianus cultured with lactate as the sole carbon source. The optimal growth and released protein in lactate culture were determined under different pH conditions, and the LC–MS/MS-identified proteins were associated with the tricarboxylic acid cycle, glycolysis pathway, and cellular stress responses in cells, indicating that autolysis of K. marxianus had occurred under the culture conditions. The abundant glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) was cocrystallized with other proteins in the cell-free fraction, and the low transcription level of the GAP1 gene indicated that the protein abundance under autolysis conditions was dependent on protein stability. These results suggest that lactate induces the growth and autolysis of K. marxianus, releasing proteins and peptides. These findings can be fundamental for K. marxianus probiotic and kefir studies in the future.Shou-Chen LoChia-Yin YangDony Chacko MathewChieh-Chen HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shou-Chen Lo Chia-Yin Yang Dony Chacko Mathew Chieh-Chen Huang Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture
description	Abstract Kluyveromyces marxianus is a yeast that could be identified from kefir and can use a broad range of substrates, such as glucose and lactate, as carbon sources. The lactate produced in kefir culture can be a substrate for K. marxianus. However, the complexity of the kefir microbiota makes the traits of K. marxianus difficult to study. In this research, we focused on K. marxianus cultured with lactate as the sole carbon source. The optimal growth and released protein in lactate culture were determined under different pH conditions, and the LC–MS/MS-identified proteins were associated with the tricarboxylic acid cycle, glycolysis pathway, and cellular stress responses in cells, indicating that autolysis of K. marxianus had occurred under the culture conditions. The abundant glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) was cocrystallized with other proteins in the cell-free fraction, and the low transcription level of the GAP1 gene indicated that the protein abundance under autolysis conditions was dependent on protein stability. These results suggest that lactate induces the growth and autolysis of K. marxianus, releasing proteins and peptides. These findings can be fundamental for K. marxianus probiotic and kefir studies in the future.
format	article
author	Shou-Chen Lo Chia-Yin Yang Dony Chacko Mathew Chieh-Chen Huang
author_facet	Shou-Chen Lo Chia-Yin Yang Dony Chacko Mathew Chieh-Chen Huang
author_sort	Shou-Chen Lo
title	Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture
title_short	Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture
title_full	Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture
title_fullStr	Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture
title_full_unstemmed	Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture
title_sort	growth and autolysis of the kefir yeast kluyveromyces marxianus in lactate culture
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/95436ba6c6fe4f359e3699ef64a6388e
work_keys_str_mv	AT shouchenlo growthandautolysisofthekefiryeastkluyveromycesmarxianusinlactateculture AT chiayinyang growthandautolysisofthekefiryeastkluyveromycesmarxianusinlactateculture AT donychackomathew growthandautolysisofthekefiryeastkluyveromycesmarxianusinlactateculture AT chiehchenhuang growthandautolysisofthekefiryeastkluyveromycesmarxianusinlactateculture
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Growth and autolysis of the kefir yeast Kluyveromyces marxianus in lactate culture

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