Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats

Abstract Cholestasis is a clinical disorder defined as an impairment of bile flow, and that leads to toxic bile acid (BA) accumulation in hepatocytes. Here, we investigated the hepatoprotective effect of Yinchenhaotang (YCHT), a well-known formulae for the treatment of jaundice and liver disorders,...

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Autores principales:	Jingyu Yan, Guoxiang Xie, Chungeng Liang, Yiyang Hu, Aihua Zhao, Fengjie Huang, Ping Hu, Ping Liu, Wei Jia, Xiaoning Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/954acb8f56a443e08fea497cc61db122
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spelling	oai:doaj.org-article:954acb8f56a443e08fea497cc61db1222021-12-02T16:06:29ZHerbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats10.1038/s41598-017-04536-52045-2322https://doaj.org/article/954acb8f56a443e08fea497cc61db1222017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04536-5https://doaj.org/toc/2045-2322Abstract Cholestasis is a clinical disorder defined as an impairment of bile flow, and that leads to toxic bile acid (BA) accumulation in hepatocytes. Here, we investigated the hepatoprotective effect of Yinchenhaotang (YCHT), a well-known formulae for the treatment of jaundice and liver disorders, against the cholestasis using the α-naphthylisothiocyanate (ANIT)-induced cholestasis in male Wistar rats. ANIT feeding induced significant cholestasis with substantially increased intrahepatic retention of hydrophobic BAs. The dynamic changes of serum and liver BAs indicated that YCHT was able to attenuate ANIT-induced BA perturbation, which is consistent with the histopathological findings that YCHT significantly decreased the liver damage. YCHT treatment substantially reduced serum alanine aminotransferase (ALT), alkaline phosphatase (AST), total bilirubin (TBIL) and direct bilirubin (DBIL) with minimal bile duct damage in the ANIT treated rats. Elevated mRNA expression of liver IL-6, IL-17A, IL-17F, TGF-β1, α-SMA, TGR5, NTCP, OATP1a1, and ileum ASBT and decreased liver IL-10, FXR, CAR, VDR, BSEP, MRP2, MRP3, MRP4 was also observed in ANIT-induced cholestasis but were attenuated or normalized by YCHT. Our results demonstrated that the BA profiles were significantly altered with ANIT intervention and YCHT possesses the hepatoprotective potential against cholestatic liver injury induced by hepatotoxin such as ANIT.Jingyu YanGuoxiang XieChungeng LiangYiyang HuAihua ZhaoFengjie HuangPing HuPing LiuWei JiaXiaoning WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jingyu Yan Guoxiang Xie Chungeng Liang Yiyang Hu Aihua Zhao Fengjie Huang Ping Hu Ping Liu Wei Jia Xiaoning Wang Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats
description	Abstract Cholestasis is a clinical disorder defined as an impairment of bile flow, and that leads to toxic bile acid (BA) accumulation in hepatocytes. Here, we investigated the hepatoprotective effect of Yinchenhaotang (YCHT), a well-known formulae for the treatment of jaundice and liver disorders, against the cholestasis using the α-naphthylisothiocyanate (ANIT)-induced cholestasis in male Wistar rats. ANIT feeding induced significant cholestasis with substantially increased intrahepatic retention of hydrophobic BAs. The dynamic changes of serum and liver BAs indicated that YCHT was able to attenuate ANIT-induced BA perturbation, which is consistent with the histopathological findings that YCHT significantly decreased the liver damage. YCHT treatment substantially reduced serum alanine aminotransferase (ALT), alkaline phosphatase (AST), total bilirubin (TBIL) and direct bilirubin (DBIL) with minimal bile duct damage in the ANIT treated rats. Elevated mRNA expression of liver IL-6, IL-17A, IL-17F, TGF-β1, α-SMA, TGR5, NTCP, OATP1a1, and ileum ASBT and decreased liver IL-10, FXR, CAR, VDR, BSEP, MRP2, MRP3, MRP4 was also observed in ANIT-induced cholestasis but were attenuated or normalized by YCHT. Our results demonstrated that the BA profiles were significantly altered with ANIT intervention and YCHT possesses the hepatoprotective potential against cholestatic liver injury induced by hepatotoxin such as ANIT.
format	article
author	Jingyu Yan Guoxiang Xie Chungeng Liang Yiyang Hu Aihua Zhao Fengjie Huang Ping Hu Ping Liu Wei Jia Xiaoning Wang
author_facet	Jingyu Yan Guoxiang Xie Chungeng Liang Yiyang Hu Aihua Zhao Fengjie Huang Ping Hu Ping Liu Wei Jia Xiaoning Wang
author_sort	Jingyu Yan
title	Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats
title_short	Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats
title_full	Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats
title_fullStr	Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats
title_full_unstemmed	Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats
title_sort	herbal medicine yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/954acb8f56a443e08fea497cc61db122
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Herbal medicine Yinchenhaotang protects against α-naphthylisothiocyanate-induced cholestasis in rats

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