Calcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes

Szu-Yuan Wu,1–4 Li-Ting Chang,5 Sydeny Peng,6 Hsieh-Chih Tsai5 1Institute of Toxicology, College of Medicine, National Taiwan University, 2Department of Radiation Oncology, Wan Fang Hospital, Taipei Medical University, 3Department of Internal Medicine, School of Medicine, College of Medi...

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Autores principales:	Wu SY, Chang LT, Peng S, Tsai HC
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/955137204700417c87150bed35010b39
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spelling	oai:doaj.org-article:955137204700417c87150bed35010b392021-12-02T07:46:33ZCalcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes1178-2013https://doaj.org/article/955137204700417c87150bed35010b392015-02-01T00:00:00Zhttp://www.dovepress.com/calcium-activated-gene-transfection-from-dnapolyamic-acid-co-imide-com-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Szu-Yuan Wu,1–4 Li-Ting Chang,5 Sydeny Peng,6 Hsieh-Chih Tsai5 1Institute of Toxicology, College of Medicine, National Taiwan University, 2Department of Radiation Oncology, Wan Fang Hospital, Taipei Medical University, 3Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; 4Department of Biotechnology, Hungkuang University, Taichung, Taiwan; 5Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan; 6Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan Abstract: In this study, we synthesized a water-soluble poly(amic acid-co-imide) (PA-I) from ethylenediaminetetraacetic dianhydride (EDTA) and 2,2'-(ethylenedioxy)bis(ethylamine) that possesses comparable transfection efficiency to that of polyethylenimine (PEI), when prepared in combination with divalent calcium cations. The polycondensation of monomers afforded poly(amic acid) (PA) precursors, and subsequent thermal imidization resulted in the formation of PA-I. At a polymer/DNA ratio (indicated by the molar ratio of nitrogen in the polymer to phosphate in DNA) of 40, complete retardation of the DNA band was observed by gel electrophoresis, indicating the strong association of DNA with PA-I. A zeta potential of -22 mV was recorded for the PA-I polymer solution, and no apparent cytotoxicity was observed at concentrations up to 500 µg·mL-1. In the presence of divalent Ca2+, the transfection efficiency of PA-I was higher than that of PA, due to the formation of a copolymer/Ca2+/DNA polyplex and the reduction in negative charge due to thermal cyclization. Interestingly, a synergistic effect of Ca2+ and the synthesized copolymer on DNA transfection was observed. The use of Ca2+ or copolymer alone resulted in unsatisfactory delivery, whereas the formation of three-component polyplexes synergistically increased DNA transfection. Our findings demonstrated that a PA-I/Ca2+/DNA polyplex could serve as a promising candidate for gene delivery. Keywords: gene carrier, poly(amic acid), polyimideWu SYChang LTPeng STsai HCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1637-1647 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Wu SY Chang LT Peng S Tsai HC Calcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes
description	Szu-Yuan Wu,1–4 Li-Ting Chang,5 Sydeny Peng,6 Hsieh-Chih Tsai5 1Institute of Toxicology, College of Medicine, National Taiwan University, 2Department of Radiation Oncology, Wan Fang Hospital, Taipei Medical University, 3Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; 4Department of Biotechnology, Hungkuang University, Taichung, Taiwan; 5Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan; 6Department of Chemical Engineering, National Tsing Hua University, Hsinchu, Taiwan Abstract: In this study, we synthesized a water-soluble poly(amic acid-co-imide) (PA-I) from ethylenediaminetetraacetic dianhydride (EDTA) and 2,2'-(ethylenedioxy)bis(ethylamine) that possesses comparable transfection efficiency to that of polyethylenimine (PEI), when prepared in combination with divalent calcium cations. The polycondensation of monomers afforded poly(amic acid) (PA) precursors, and subsequent thermal imidization resulted in the formation of PA-I. At a polymer/DNA ratio (indicated by the molar ratio of nitrogen in the polymer to phosphate in DNA) of 40, complete retardation of the DNA band was observed by gel electrophoresis, indicating the strong association of DNA with PA-I. A zeta potential of -22 mV was recorded for the PA-I polymer solution, and no apparent cytotoxicity was observed at concentrations up to 500 µg·mL-1. In the presence of divalent Ca2+, the transfection efficiency of PA-I was higher than that of PA, due to the formation of a copolymer/Ca2+/DNA polyplex and the reduction in negative charge due to thermal cyclization. Interestingly, a synergistic effect of Ca2+ and the synthesized copolymer on DNA transfection was observed. The use of Ca2+ or copolymer alone resulted in unsatisfactory delivery, whereas the formation of three-component polyplexes synergistically increased DNA transfection. Our findings demonstrated that a PA-I/Ca2+/DNA polyplex could serve as a promising candidate for gene delivery. Keywords: gene carrier, poly(amic acid), polyimide
format	article
author	Wu SY Chang LT Peng S Tsai HC
author_facet	Wu SY Chang LT Peng S Tsai HC
author_sort	Wu SY
title	Calcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes
title_short	Calcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes
title_full	Calcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes
title_fullStr	Calcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes
title_full_unstemmed	Calcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes
title_sort	calcium-activated gene transfection from dna/poly(amic acid-co-imide) complexes
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/955137204700417c87150bed35010b39
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Calcium-activated gene transfection from DNA/poly(amic acid-co-imide) complexes

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