Association between dental pulp stones and calcifying nanoparticles

Jinfeng Zeng1*, Fang Yang1*, Wei Zhang2, Qimei Gong1, Yu Du1, Junqi Ling11Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology and Institute of Stomatological Research, Sun Yat-sen University, Guangzhou, China; 2National Neuroscience Institute, Singapore *Th...

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Autores principales:	Jinfeng Zeng, Fang Yang, Wei Zhang, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9553d5f2e40148b98199c42e84514551
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spelling	oai:doaj.org-article:9553d5f2e40148b98199c42e845145512021-12-02T01:35:39ZAssociation between dental pulp stones and calcifying nanoparticles1176-91141178-2013https://doaj.org/article/9553d5f2e40148b98199c42e845145512011-01-01T00:00:00Zhttp://www.dovepress.com/association-between-dental-pulp-stones-and-calcifying-nanoparticles-a6015https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jinfeng Zeng1, Fang Yang1, Wei Zhang2, Qimei Gong1, Yu Du1, Junqi Ling11Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology and Institute of Stomatological Research, Sun Yat-sen University, Guangzhou, China; 2National Neuroscience Institute, Singapore *These authors contributed equally to this workAbstract: The etiology of dental pulp stones, one type of extraskeletal calcification disease, remains elusive to date. Calcifying nanoparticles (CNPs), formerly referred to as nanobacteria, were reported to be one etiological factor in a number of extraskeletal calcification diseases. We hypothesized that CNPs are involved in the calcification of the dental pulp tissue, and therefore investigated the link between CNPs and dental pulp stones. Sixty-five freshly collected dental pulp stones, each from a different patient, were analyzed. Thirteen of the pulp stones were examined for the existence of CNPs in situ by immunohistochemical staining (IHS), indirect immunofluorescence staining (IIFS), and transmission electron microscope (TEM). The remaining 52 pulp stones were used for isolation and cultivation of CNPs; the cultured CNPs were identified and confirmed via their shape and growth characteristics. Among the dental pulp stones examined in situ, 84.6% of the tissue samples staines positive for CNPs antigen by IHS; the corresponding rate by IIFS was 92.3 %. In 88.2% of the cultured samples, CNPs were isolated and cultivated successfully. The CNPs were visible under TEM as 200&ndash;400 nm diameter spherical particles surrounded by a compact crust. CNPs could be detected and isolated from a high percentage of dental pulp stones, suggesting that CNPs might play an important role in the calcification of dental pulp.Keywords: calcifying nanoparticles, nanobacteria, pulp stones Jinfeng ZengFang YangWei Zhanget alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 109-118 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Jinfeng Zeng Fang Yang Wei Zhang et al Association between dental pulp stones and calcifying nanoparticles
description	Jinfeng Zeng1, Fang Yang1, Wei Zhang2, Qimei Gong1, Yu Du1, Junqi Ling11Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology and Institute of Stomatological Research, Sun Yat-sen University, Guangzhou, China; 2National Neuroscience Institute, Singapore *These authors contributed equally to this workAbstract: The etiology of dental pulp stones, one type of extraskeletal calcification disease, remains elusive to date. Calcifying nanoparticles (CNPs), formerly referred to as nanobacteria, were reported to be one etiological factor in a number of extraskeletal calcification diseases. We hypothesized that CNPs are involved in the calcification of the dental pulp tissue, and therefore investigated the link between CNPs and dental pulp stones. Sixty-five freshly collected dental pulp stones, each from a different patient, were analyzed. Thirteen of the pulp stones were examined for the existence of CNPs in situ by immunohistochemical staining (IHS), indirect immunofluorescence staining (IIFS), and transmission electron microscope (TEM). The remaining 52 pulp stones were used for isolation and cultivation of CNPs; the cultured CNPs were identified and confirmed via their shape and growth characteristics. Among the dental pulp stones examined in situ, 84.6% of the tissue samples staines positive for CNPs antigen by IHS; the corresponding rate by IIFS was 92.3 %. In 88.2% of the cultured samples, CNPs were isolated and cultivated successfully. The CNPs were visible under TEM as 200&ndash;400 nm diameter spherical particles surrounded by a compact crust. CNPs could be detected and isolated from a high percentage of dental pulp stones, suggesting that CNPs might play an important role in the calcification of dental pulp.Keywords: calcifying nanoparticles, nanobacteria, pulp stones
format	article
author	Jinfeng Zeng Fang Yang Wei Zhang et al
author_facet	Jinfeng Zeng Fang Yang Wei Zhang et al
author_sort	Jinfeng Zeng
title	Association between dental pulp stones and calcifying nanoparticles
title_short	Association between dental pulp stones and calcifying nanoparticles
title_full	Association between dental pulp stones and calcifying nanoparticles
title_fullStr	Association between dental pulp stones and calcifying nanoparticles
title_full_unstemmed	Association between dental pulp stones and calcifying nanoparticles
title_sort	association between dental pulp stones and calcifying nanoparticles
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/9553d5f2e40148b98199c42e84514551
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Association between dental pulp stones and calcifying nanoparticles

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