Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)

The objective of the current experiment was to study the effect of different avian egg yolks (EYs) on rams epididymal sperms stored at 4 ºC. Sixteen pairs of testes (n=32) were mature Awassi rams of about 1-4 years, slaughtered at Al-Sadoon abattoir, Mosul city were collected. The spermatozoa were...

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Autores principales:	Faten Fadhel Mustafa, Uday Talat Naoman
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	avian egg yolk epididymal sperm ram semen Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.88532.1096 https://doaj.org/article/955513e60b7c4e5d83cfddd9a7f74213
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spelling	oai:doaj.org-article:955513e60b7c4e5d83cfddd9a7f742132021-12-02T17:12:50ZComparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)https://dx.doi.org/10.21608/javs.2021.88532.10961687-40722090-3308https://doaj.org/article/955513e60b7c4e5d83cfddd9a7f742132021-10-01T00:00:00Zhttps://javs.journals.ekb.eg/article_192839.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308The objective of the current experiment was to study the effect of different avian egg yolks (EYs) on rams epididymal sperms stored at 4 ºC. Sixteen pairs of testes (n=32) were mature Awassi rams of about 1-4 years, slaughtered at Al-Sadoon abattoir, Mosul city were collected. The spermatozoa were collected from the tail of the epididymis by cutting and squeezing; after that, a total of 0.5 ml of spermatozoa volume was diluted by using sodium citrate 2.9% and the volume was completed to 1 ml, then the sample was divided into four aliquots (0.25 ml for each one). The extender consisted of egg yolk (EY) 10% of four avian types (chicken, quail, duck, turkey), sodium citrate 2.9%, fructose 2.4gm, penicillin, and streptomycin (100.000 IU and 100 mg, respectively) in 100 distal water. Sperms individual motility, live sperm percentage, sperms abnormalities were checked at 0, 72, 144 hours of storage. Results of the present study revealed that quail egg yolk improved sperms, individual motility, live sperm percentage with longer life span and reduce sperms abnormalities, followed by Turkey, chicken and duck EYs, respectively. Individual motility of the spermatozoa after 144 h of storage in quail EY extender were (36.00%± 1.20), which higher than turkey EY (32.30%± 1.30). However, these results were higher and significant (P˂ 0.05) when compared with chicken EY extender (24.60%± 2.30) and duck EY extender (19.00%± 1.50). Similar results of quail and turkey EYs were manifested in the percentage of live spermatozoa, which were (38.00%± 1.20), (33.30%± 1.30), (24.00%± 2.30), and (19.90%± 1.50), respectively. The duck EY was significantly lower (P˂ 0.05) when comparing the four egg yolks types after storage for 144 h. In conclusion: we advise potentially using a quail or turkey EYs extender for the chilled storage for ram epididymal sperms. Faten Fadhel MustafaUday Talat NaomanEgyptian Society for Animal Managementarticleavian egg yolkepididymal spermramsemenZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 4, Pp 46-50 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	avian egg yolk epididymal sperm ram semen Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	avian egg yolk epididymal sperm ram semen Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Faten Fadhel Mustafa Uday Talat Naoman Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)
description	The objective of the current experiment was to study the effect of different avian egg yolks (EYs) on rams epididymal sperms stored at 4 ºC. Sixteen pairs of testes (n=32) were mature Awassi rams of about 1-4 years, slaughtered at Al-Sadoon abattoir, Mosul city were collected. The spermatozoa were collected from the tail of the epididymis by cutting and squeezing; after that, a total of 0.5 ml of spermatozoa volume was diluted by using sodium citrate 2.9% and the volume was completed to 1 ml, then the sample was divided into four aliquots (0.25 ml for each one). The extender consisted of egg yolk (EY) 10% of four avian types (chicken, quail, duck, turkey), sodium citrate 2.9%, fructose 2.4gm, penicillin, and streptomycin (100.000 IU and 100 mg, respectively) in 100 distal water. Sperms individual motility, live sperm percentage, sperms abnormalities were checked at 0, 72, 144 hours of storage. Results of the present study revealed that quail egg yolk improved sperms, individual motility, live sperm percentage with longer life span and reduce sperms abnormalities, followed by Turkey, chicken and duck EYs, respectively. Individual motility of the spermatozoa after 144 h of storage in quail EY extender were (36.00%± 1.20), which higher than turkey EY (32.30%± 1.30). However, these results were higher and significant (P˂ 0.05) when compared with chicken EY extender (24.60%± 2.30) and duck EY extender (19.00%± 1.50). Similar results of quail and turkey EYs were manifested in the percentage of live spermatozoa, which were (38.00%± 1.20), (33.30%± 1.30), (24.00%± 2.30), and (19.90%± 1.50), respectively. The duck EY was significantly lower (P˂ 0.05) when comparing the four egg yolks types after storage for 144 h. In conclusion: we advise potentially using a quail or turkey EYs extender for the chilled storage for ram epididymal sperms.
format	article
author	Faten Fadhel Mustafa Uday Talat Naoman
author_facet	Faten Fadhel Mustafa Uday Talat Naoman
author_sort	Faten Fadhel Mustafa
title	Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)
title_short	Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)
title_full	Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)
title_fullStr	Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)
title_full_unstemmed	Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)
title_sort	comparative study of using four types of avian egg yolk for epididymal sperms chilled storage in awassi rams (ovis aries)
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.88532.1096 https://doaj.org/article/955513e60b7c4e5d83cfddd9a7f74213
work_keys_str_mv	AT fatenfadhelmustafa comparativestudyofusingfourtypesofavianeggyolkforepididymalspermschilledstorageinawassiramsovisaries AT udaytalatnaoman comparativestudyofusingfourtypesofavianeggyolkforepididymalspermschilledstorageinawassiramsovisaries
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Comparative Study of Using Four Types of Avian Egg Yolk for Epididymal Sperms Chilled Storage in Awassi Rams (Ovis aries)

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