Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA
Abstract Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibio...
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Nature Portfolio
2017
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oai:doaj.org-article:95566b469c67471991905cef023d55b12021-12-02T11:52:19ZAntibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA10.1038/s41598-017-08392-12045-2322https://doaj.org/article/95566b469c67471991905cef023d55b12017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08392-1https://doaj.org/toc/2045-2322Abstract Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against Mycoplasma contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells. Unexpectedly, here we found that ciprofloxacin induced the release of both DNA (mitochondrial and chromosomal sequences) and DNA-binding proteins on the exofacial surfaces of small extracellular vesicles referred to in this paper as exosomes. Furthermore, a label-free optical biosensor analysis revealed DNA-dependent binding of exosomes to fibronectin. DNA release on the surface of exosomes was not affected any further by cellular activation or apoptosis induction. Our results reveal for the first time that prolonged low-dose ciprofloxacin exposure leads to the release of DNA associated with the external surface of exosomes.Andrea NémethNorbert OrgovanBarbara W SódarXabier OsteikoetxeaKrisztina PálócziKatalin É. Szabó-TaylorKrisztina V. VukmanÁgnes KittelLilla TuriákZoltán WienerSára TóthLászló DrahosKároly VékeyRobert HorvathEdit I. BuzásNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017) |
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Medicine R Science Q Andrea Németh Norbert Orgovan Barbara W Sódar Xabier Osteikoetxea Krisztina Pálóczi Katalin É. Szabó-Taylor Krisztina V. Vukman Ágnes Kittel Lilla Turiák Zoltán Wiener Sára Tóth László Drahos Károly Vékey Robert Horvath Edit I. Buzás Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA |
| description |
Abstract Recently, biological roles of extracellular vesicles (which include among others exosomes, microvesicles and apoptotic bodies) have attracted substantial attention in various fields of biomedicine. Here we investigated the impact of sustained exposure of cells to the fluoroquinolone antibiotic ciprofloxacin on the released extracellular vesicles. Ciprofloxacin is widely used in humans against bacterial infections as well as in cell cultures against Mycoplasma contamination. However, ciprofloxacin is an inducer of oxidative stress and mitochondrial dysfunction of mammalian cells. Unexpectedly, here we found that ciprofloxacin induced the release of both DNA (mitochondrial and chromosomal sequences) and DNA-binding proteins on the exofacial surfaces of small extracellular vesicles referred to in this paper as exosomes. Furthermore, a label-free optical biosensor analysis revealed DNA-dependent binding of exosomes to fibronectin. DNA release on the surface of exosomes was not affected any further by cellular activation or apoptosis induction. Our results reveal for the first time that prolonged low-dose ciprofloxacin exposure leads to the release of DNA associated with the external surface of exosomes. |
| format |
article |
| author |
Andrea Németh Norbert Orgovan Barbara W Sódar Xabier Osteikoetxea Krisztina Pálóczi Katalin É. Szabó-Taylor Krisztina V. Vukman Ágnes Kittel Lilla Turiák Zoltán Wiener Sára Tóth László Drahos Károly Vékey Robert Horvath Edit I. Buzás |
| author_facet |
Andrea Németh Norbert Orgovan Barbara W Sódar Xabier Osteikoetxea Krisztina Pálóczi Katalin É. Szabó-Taylor Krisztina V. Vukman Ágnes Kittel Lilla Turiák Zoltán Wiener Sára Tóth László Drahos Károly Vékey Robert Horvath Edit I. Buzás |
| author_sort |
Andrea Németh |
| title |
Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA |
| title_short |
Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA |
| title_full |
Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA |
| title_fullStr |
Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA |
| title_full_unstemmed |
Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA |
| title_sort |
antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated dna |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/95566b469c67471991905cef023d55b1 |
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