Purification of Curcuminoids from Natural Deep Eutectic Solvents (NADES) Matrices Using Chromatography-Based Separation Methods

Curcuminoids can be successfully extracted from Curcuma zedoaria using natural deep eutectic solvents (NADES) as extraction solvents. However, a mixture of extracted curcuminoids, NADES, and impurities from C. zedoaria was obtained as a slurry at the end of the extraction process. Therefore, further...

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Autores principales:	Orchidea Rachmaniah, Muhammad Rifqy Muhsin, Angga Widya Putra, Muhammad Rachimoellah
Formato:	article
Lenguaje:	EN
Publicado:	Department of Chemistry, Universitas Gadjah Mada 2021
Materias:	curcumin chromatography recrystallization separation solid phase extraction Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/955d12d5e5f04e8ea23d558bb539b4c1
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spelling	oai:doaj.org-article:955d12d5e5f04e8ea23d558bb539b4c12021-12-02T17:58:05ZPurification of Curcuminoids from Natural Deep Eutectic Solvents (NADES) Matrices Using Chromatography-Based Separation Methods1411-94202460-157810.22146/ijc.58935https://doaj.org/article/955d12d5e5f04e8ea23d558bb539b4c12021-08-01T00:00:00Zhttps://jurnal.ugm.ac.id/ijc/article/view/58935https://doaj.org/toc/1411-9420https://doaj.org/toc/2460-1578Curcuminoids can be successfully extracted from Curcuma zedoaria using natural deep eutectic solvents (NADES) as extraction solvents. However, a mixture of extracted curcuminoids, NADES, and impurities from C. zedoaria was obtained as a slurry at the end of the extraction process. Therefore, further separation and purification were required to obtain the extracted compound in high purity. Herein, two purification methods based on classical column chromatography (CCC) and solid-phase extraction (SPE), were evaluated for the purification of curcuminoids from NADES matrices after extraction. Choline chloride–malic acid–water (CCMA–H2O) and choline chloride–citric acid–water (CCCA–H2O) in the molar ratio of 1:1:18 were selected as NADES matrices due to their high solubility and stabilization capability for curcuminoids. Ethanol-conditioned silica gel (60–200 µm) was applied as the bed resin for CCC, and a C18 cartridge was used for SPE. Acetonitrile/0.1% acetic acid, water/0.1% acetic acid, and iso-propanol/0.1% acetic acid were used as mobile phases for CCC. For SPE, methanol/0.05% acetic acid and water/0.05% acetic acid were applied in the conditioning step, water/0.05% acetic acid in the washing step, and methanol/acetonitrile (1:1) in the eluting step. The SPE method produced higher recovery of curcuminoids from the CCCA–H2O and CCMA–H2O matrices (75.27% and 73.40%, respectively) compared to CCC (51.9% and 61.0%, respectively). After removing the NADES constituents from the crude extract of curcuminoids, recrystallization was attempted.Orchidea RachmaniahMuhammad Rifqy MuhsinAngga Widya PutraMuhammad RachimoellahDepartment of Chemistry, Universitas Gadjah Madaarticlecurcuminchromatographyrecrystallizationseparationsolid phase extractionChemistryQD1-999ENIndonesian Journal of Chemistry, Vol 21, Iss 4, Pp 806-815 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	curcumin chromatography recrystallization separation solid phase extraction Chemistry QD1-999
spellingShingle	curcumin chromatography recrystallization separation solid phase extraction Chemistry QD1-999 Orchidea Rachmaniah Muhammad Rifqy Muhsin Angga Widya Putra Muhammad Rachimoellah Purification of Curcuminoids from Natural Deep Eutectic Solvents (NADES) Matrices Using Chromatography-Based Separation Methods
description	Curcuminoids can be successfully extracted from Curcuma zedoaria using natural deep eutectic solvents (NADES) as extraction solvents. However, a mixture of extracted curcuminoids, NADES, and impurities from C. zedoaria was obtained as a slurry at the end of the extraction process. Therefore, further separation and purification were required to obtain the extracted compound in high purity. Herein, two purification methods based on classical column chromatography (CCC) and solid-phase extraction (SPE), were evaluated for the purification of curcuminoids from NADES matrices after extraction. Choline chloride–malic acid–water (CCMA–H2O) and choline chloride–citric acid–water (CCCA–H2O) in the molar ratio of 1:1:18 were selected as NADES matrices due to their high solubility and stabilization capability for curcuminoids. Ethanol-conditioned silica gel (60–200 µm) was applied as the bed resin for CCC, and a C18 cartridge was used for SPE. Acetonitrile/0.1% acetic acid, water/0.1% acetic acid, and iso-propanol/0.1% acetic acid were used as mobile phases for CCC. For SPE, methanol/0.05% acetic acid and water/0.05% acetic acid were applied in the conditioning step, water/0.05% acetic acid in the washing step, and methanol/acetonitrile (1:1) in the eluting step. The SPE method produced higher recovery of curcuminoids from the CCCA–H2O and CCMA–H2O matrices (75.27% and 73.40%, respectively) compared to CCC (51.9% and 61.0%, respectively). After removing the NADES constituents from the crude extract of curcuminoids, recrystallization was attempted.
format	article
author	Orchidea Rachmaniah Muhammad Rifqy Muhsin Angga Widya Putra Muhammad Rachimoellah
author_facet	Orchidea Rachmaniah Muhammad Rifqy Muhsin Angga Widya Putra Muhammad Rachimoellah
author_sort	Orchidea Rachmaniah
title	Purification of Curcuminoids from Natural Deep Eutectic Solvents (NADES) Matrices Using Chromatography-Based Separation Methods
title_short	Purification of Curcuminoids from Natural Deep Eutectic Solvents (NADES) Matrices Using Chromatography-Based Separation Methods
title_full	Purification of Curcuminoids from Natural Deep Eutectic Solvents (NADES) Matrices Using Chromatography-Based Separation Methods
title_fullStr	Purification of Curcuminoids from Natural Deep Eutectic Solvents (NADES) Matrices Using Chromatography-Based Separation Methods
title_full_unstemmed	Purification of Curcuminoids from Natural Deep Eutectic Solvents (NADES) Matrices Using Chromatography-Based Separation Methods
title_sort	purification of curcuminoids from natural deep eutectic solvents (nades) matrices using chromatography-based separation methods
publisher	Department of Chemistry, Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/955d12d5e5f04e8ea23d558bb539b4c1
work_keys_str_mv	AT orchidearachmaniah purificationofcurcuminoidsfromnaturaldeepeutecticsolventsnadesmatricesusingchromatographybasedseparationmethods AT muhammadrifqymuhsin purificationofcurcuminoidsfromnaturaldeepeutecticsolventsnadesmatricesusingchromatographybasedseparationmethods AT anggawidyaputra purificationofcurcuminoidsfromnaturaldeepeutecticsolventsnadesmatricesusingchromatographybasedseparationmethods AT muhammadrachimoellah purificationofcurcuminoidsfromnaturaldeepeutecticsolventsnadesmatricesusingchromatographybasedseparationmethods
_version_	1718379060259717120

Purification of Curcuminoids from Natural Deep Eutectic Solvents (NADES) Matrices Using Chromatography-Based Separation Methods

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