Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo

Abstract Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory role of ficolins has been sugg...

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Autores principales:	Ninette Genster, Olga Østrup, Camilla Schjalm, Tom Eirik Mollnes, Jack B. Cowland, Peter Garred
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9565494a7e254c2098c2868b24f37f7c
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spelling	oai:doaj.org-article:9565494a7e254c2098c2868b24f37f7c2021-12-02T15:05:10ZFicolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo10.1038/s41598-017-04121-w2045-2322https://doaj.org/article/9565494a7e254c2098c2868b24f37f7c2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04121-whttps://doaj.org/toc/2045-2322Abstract Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory role of ficolins has been suggested. Yet, the contribution of ficolins to inflammatory disease processes remains elusive. To address this, we investigated ficolin deficient mice during a lipopolysaccharide (LPS)-induced model of systemic inflammation. Although murine serum ficolin was shown to bind LPS in vitro, there was no difference between wildtype and ficolin deficient mice in morbidity and mortality by LPS-induced inflammation. Moreover, there was no difference between wildtype and ficolin deficient mice in the inflammatory cytokine profiles after LPS challenge. These findings were substantiated by microarray analysis revealing an unaltered spleen transcriptome profile in ficolin deficient mice compared to wildtype mice. Collectively, results from this study demonstrate that ficolins are not involved in host response to LPS-induced systemic inflammation.Ninette GensterOlga ØstrupCamilla SchjalmTom Eirik MollnesJack B. CowlandPeter GarredNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ninette Genster Olga Østrup Camilla Schjalm Tom Eirik Mollnes Jack B. Cowland Peter Garred Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo
description	Abstract Ficolins are a family of pattern recognition molecules that are capable of activating the lectin pathway of complement. A limited number of reports have demonstrated a protective role of ficolins in animal models of infection. In addition, an immune modulatory role of ficolins has been suggested. Yet, the contribution of ficolins to inflammatory disease processes remains elusive. To address this, we investigated ficolin deficient mice during a lipopolysaccharide (LPS)-induced model of systemic inflammation. Although murine serum ficolin was shown to bind LPS in vitro, there was no difference between wildtype and ficolin deficient mice in morbidity and mortality by LPS-induced inflammation. Moreover, there was no difference between wildtype and ficolin deficient mice in the inflammatory cytokine profiles after LPS challenge. These findings were substantiated by microarray analysis revealing an unaltered spleen transcriptome profile in ficolin deficient mice compared to wildtype mice. Collectively, results from this study demonstrate that ficolins are not involved in host response to LPS-induced systemic inflammation.
format	article
author	Ninette Genster Olga Østrup Camilla Schjalm Tom Eirik Mollnes Jack B. Cowland Peter Garred
author_facet	Ninette Genster Olga Østrup Camilla Schjalm Tom Eirik Mollnes Jack B. Cowland Peter Garred
author_sort	Ninette Genster
title	Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo
title_short	Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo
title_full	Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo
title_fullStr	Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo
title_full_unstemmed	Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo
title_sort	ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9565494a7e254c2098c2868b24f37f7c
work_keys_str_mv	AT ninettegenster ficolinsdonotalterhostimmuneresponsestolipopolysaccharideinducedinflammationinvivo AT olgaøstrup ficolinsdonotalterhostimmuneresponsestolipopolysaccharideinducedinflammationinvivo AT camillaschjalm ficolinsdonotalterhostimmuneresponsestolipopolysaccharideinducedinflammationinvivo AT tomeirikmollnes ficolinsdonotalterhostimmuneresponsestolipopolysaccharideinducedinflammationinvivo AT jackbcowland ficolinsdonotalterhostimmuneresponsestolipopolysaccharideinducedinflammationinvivo AT petergarred ficolinsdonotalterhostimmuneresponsestolipopolysaccharideinducedinflammationinvivo
_version_	1718388907354095616

Ficolins do not alter host immune responses to lipopolysaccharide-induced inflammation in vivo

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