Identification of key genes involved in recovery from spinal cord injury in adult zebrafish

Zebrafish are an effective vertebrate model to study the mechanisms underlying recovery after spinal cord injury. The subacute phase after spinal cord injury is critical to the recovery of neurological function, which involves tissue bridging and axon regeneration. In this study, we found that zebra...

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Autores principales:	Wen-Yuan Shen, Xuan-Hao Fu, Jun Cai, Wen-Chang Li, Bao-You Fan, Yi-Lin Pang, Chen-Xi Zhao, Muhtidir Abula, Xiao-Hong Kong, Xue Yao, Shi-Qing Feng
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2022
Materias:	axon regeneration; clasp2; endogenous neural stem cells; h1m; microtubule; nanog; neural regeneration; neurogenesis; spinal cord injury; subacute phase Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/956b2ff7946c483892189033fa9dda87
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spelling	oai:doaj.org-article:956b2ff7946c483892189033fa9dda872021-11-19T12:16:44ZIdentification of key genes involved in recovery from spinal cord injury in adult zebrafish1673-537410.4103/1673-5374.327360https://doaj.org/article/956b2ff7946c483892189033fa9dda872022-01-01T00:00:00Zhttp://www.nrronline.org/article.asp?issn=1673-5374;year=2022;volume=17;issue=6;spage=1334;epage=1342;aulast=Shenhttps://doaj.org/toc/1673-5374Zebrafish are an effective vertebrate model to study the mechanisms underlying recovery after spinal cord injury. The subacute phase after spinal cord injury is critical to the recovery of neurological function, which involves tissue bridging and axon regeneration. In this study, we found that zebrafish spontaneously recovered 44% of their swimming ability within the subacute phase (2 weeks) after spinal cord injury. During this period, we identified 7762 differentially expressed genes in spinal cord tissue: 2950 were up-regulated and 4812 were down-regulated. These differentially expressed genes were primarily concentrated in the biological processes of the respiratory chain, axon regeneration, and cell-component morphogenesis. The genes were also mostly involved in the regulation of metabolic pathways, the cell cycle, and gene-regulation pathways. We verified the gene expression of two differentially expressed genes, clasp2 up-regulation and h1m down-regulation, in zebrafish spinal cord tissue in vitro. Pathway enrichment analysis revealed that up-regulated clasp2 functions similarly to microtubule-associated protein, which is responsible for axon extension regulated by microtubules. Down-regulated h1m controls endogenous stem cell differentiation after spinal cord injury. This study provides new candidate genes, clasp2 and h1m, as potential therapeutic intervention targets for spinal cord injury repair by neuroregeneration. All experimental procedures and protocols were approved by the Animal Ethics Committee of Tianjin Institute of Medical & Pharmaceutical Sciences (approval No. IMPS-EAEP-Q-2019-02) on September 24, 2019.Wen-Yuan ShenXuan-Hao FuJun CaiWen-Chang LiBao-You FanYi-Lin PangChen-Xi ZhaoMuhtidir AbulaXiao-Hong KongXue YaoShi-Qing FengWolters Kluwer Medknow Publicationsarticleaxon regeneration; clasp2; endogenous neural stem cells; h1m; microtubule; nanog; neural regeneration; neurogenesis; spinal cord injury; subacute phaseNeurology. Diseases of the nervous systemRC346-429ENNeural Regeneration Research, Vol 17, Iss 6, Pp 1334-1342 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	axon regeneration; clasp2; endogenous neural stem cells; h1m; microtubule; nanog; neural regeneration; neurogenesis; spinal cord injury; subacute phase Neurology. Diseases of the nervous system RC346-429
spellingShingle	axon regeneration; clasp2; endogenous neural stem cells; h1m; microtubule; nanog; neural regeneration; neurogenesis; spinal cord injury; subacute phase Neurology. Diseases of the nervous system RC346-429 Wen-Yuan Shen Xuan-Hao Fu Jun Cai Wen-Chang Li Bao-You Fan Yi-Lin Pang Chen-Xi Zhao Muhtidir Abula Xiao-Hong Kong Xue Yao Shi-Qing Feng Identification of key genes involved in recovery from spinal cord injury in adult zebrafish
description	Zebrafish are an effective vertebrate model to study the mechanisms underlying recovery after spinal cord injury. The subacute phase after spinal cord injury is critical to the recovery of neurological function, which involves tissue bridging and axon regeneration. In this study, we found that zebrafish spontaneously recovered 44% of their swimming ability within the subacute phase (2 weeks) after spinal cord injury. During this period, we identified 7762 differentially expressed genes in spinal cord tissue: 2950 were up-regulated and 4812 were down-regulated. These differentially expressed genes were primarily concentrated in the biological processes of the respiratory chain, axon regeneration, and cell-component morphogenesis. The genes were also mostly involved in the regulation of metabolic pathways, the cell cycle, and gene-regulation pathways. We verified the gene expression of two differentially expressed genes, clasp2 up-regulation and h1m down-regulation, in zebrafish spinal cord tissue in vitro. Pathway enrichment analysis revealed that up-regulated clasp2 functions similarly to microtubule-associated protein, which is responsible for axon extension regulated by microtubules. Down-regulated h1m controls endogenous stem cell differentiation after spinal cord injury. This study provides new candidate genes, clasp2 and h1m, as potential therapeutic intervention targets for spinal cord injury repair by neuroregeneration. All experimental procedures and protocols were approved by the Animal Ethics Committee of Tianjin Institute of Medical & Pharmaceutical Sciences (approval No. IMPS-EAEP-Q-2019-02) on September 24, 2019.
format	article
author	Wen-Yuan Shen Xuan-Hao Fu Jun Cai Wen-Chang Li Bao-You Fan Yi-Lin Pang Chen-Xi Zhao Muhtidir Abula Xiao-Hong Kong Xue Yao Shi-Qing Feng
author_facet	Wen-Yuan Shen Xuan-Hao Fu Jun Cai Wen-Chang Li Bao-You Fan Yi-Lin Pang Chen-Xi Zhao Muhtidir Abula Xiao-Hong Kong Xue Yao Shi-Qing Feng
author_sort	Wen-Yuan Shen
title	Identification of key genes involved in recovery from spinal cord injury in adult zebrafish
title_short	Identification of key genes involved in recovery from spinal cord injury in adult zebrafish
title_full	Identification of key genes involved in recovery from spinal cord injury in adult zebrafish
title_fullStr	Identification of key genes involved in recovery from spinal cord injury in adult zebrafish
title_full_unstemmed	Identification of key genes involved in recovery from spinal cord injury in adult zebrafish
title_sort	identification of key genes involved in recovery from spinal cord injury in adult zebrafish
publisher	Wolters Kluwer Medknow Publications
publishDate	2022
url	https://doaj.org/article/956b2ff7946c483892189033fa9dda87
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_version_	1718420085006139392

Identification of key genes involved in recovery from spinal cord injury in adult zebrafish

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