The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia

Abstract. Nisa IK, Prabaningtyas S, Lukiati B, Saptawati RT, Rodiansyah A. 2021. The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia. Biodiversitas 21: 42-49. Indonesia is one country that has water resources having an abundance of microbial...

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Autores principales:	INDAH KHOIRUN NISA, Prabaningtyas Sitoresmi, BETTY LUKIATI, RINA TRITURANI SAPTAWATI, ACHMAD RODIANSYAH
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	16s rrna gene, amylolytic bacteria, dns method, lakes, east java Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/9573c07516124947896f0ce8dd30515d
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spelling	oai:doaj.org-article:9573c07516124947896f0ce8dd30515d2021-11-22T00:53:57ZThe potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia1412-033X2085-472210.13057/biodiv/d220106https://doaj.org/article/9573c07516124947896f0ce8dd30515d2020-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6855https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Nisa IK, Prabaningtyas S, Lukiati B, Saptawati RT, Rodiansyah A. 2021. The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia. Biodiversitas 21: 42-49. Indonesia is one country that has water resources having an abundance of microbial diversity, but not explored massively. This study aims to measure the amylase activity quantitatively from 53 amylolytic bacterial isolates from Ranu Pani, Ranu Regulo, Ranu Grati, and Ngebel Lake; also it identifies the isolate with the highest amylase enzyme activity. The amylase enzyme activity test calculates with DNS (Dinitrosalycylic acid) method, molecular identification of the highest bacterial isolate is based on the 16S rRNA gene. Its relationship is determined through the phylogenetic tree with the Neighbor-Joining (NJ) method. The results showed that the fifty-three bacterial isolates have amylase activity about 0.000-0.016 units/mL. The KN bacterial isolate from Ranu Ngebel was the highest amylase activity, producing enzyme around 0.016 units/mL, while isolate G20 from Ranu Grati was the lowest, reaching about 0.0001 Unit/mL. Based on the morphological and molecular identification, the KN bacterial isolate is classified as the Bacillus cereus group with 99.4-100% sequence similarity, closely related to Bacillus paramycoides (NR 157734.1).INDAH KHOIRUN NISAPrabaningtyas SitoresmiBETTY LUKIATIRINA TRITURANI SAPTAWATIACHMAD RODIANSYAHMBI & UNS Soloarticle16s rrna gene, amylolytic bacteria, dns method, lakes, east javaBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna gene, amylolytic bacteria, dns method, lakes, east java Biology (General) QH301-705.5
spellingShingle	16s rrna gene, amylolytic bacteria, dns method, lakes, east java Biology (General) QH301-705.5 INDAH KHOIRUN NISA Prabaningtyas Sitoresmi BETTY LUKIATI RINA TRITURANI SAPTAWATI ACHMAD RODIANSYAH The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia
description	Abstract. Nisa IK, Prabaningtyas S, Lukiati B, Saptawati RT, Rodiansyah A. 2021. The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia. Biodiversitas 21: 42-49. Indonesia is one country that has water resources having an abundance of microbial diversity, but not explored massively. This study aims to measure the amylase activity quantitatively from 53 amylolytic bacterial isolates from Ranu Pani, Ranu Regulo, Ranu Grati, and Ngebel Lake; also it identifies the isolate with the highest amylase enzyme activity. The amylase enzyme activity test calculates with DNS (Dinitrosalycylic acid) method, molecular identification of the highest bacterial isolate is based on the 16S rRNA gene. Its relationship is determined through the phylogenetic tree with the Neighbor-Joining (NJ) method. The results showed that the fifty-three bacterial isolates have amylase activity about 0.000-0.016 units/mL. The KN bacterial isolate from Ranu Ngebel was the highest amylase activity, producing enzyme around 0.016 units/mL, while isolate G20 from Ranu Grati was the lowest, reaching about 0.0001 Unit/mL. Based on the morphological and molecular identification, the KN bacterial isolate is classified as the Bacillus cereus group with 99.4-100% sequence similarity, closely related to Bacillus paramycoides (NR 157734.1).
format	article
author	INDAH KHOIRUN NISA Prabaningtyas Sitoresmi BETTY LUKIATI RINA TRITURANI SAPTAWATI ACHMAD RODIANSYAH
author_facet	INDAH KHOIRUN NISA Prabaningtyas Sitoresmi BETTY LUKIATI RINA TRITURANI SAPTAWATI ACHMAD RODIANSYAH
author_sort	INDAH KHOIRUN NISA
title	The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia
title_short	The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia
title_full	The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia
title_fullStr	The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia
title_full_unstemmed	The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia
title_sort	potential of amylase enzyme activity against bacteria isolated from several lakes in east java, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/9573c07516124947896f0ce8dd30515d
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The potential of amylase enzyme activity against bacteria isolated from several lakes in East Java, Indonesia

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