Cellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis

Abstract In cnidarians, long-term ecological success relies on sexual reproduction. The sea anemone Nematostella vectensis, which has emerged as an important model organism for developmental studies, can be induced for spawning by temperature elevation and light exposure. To uncover molecular mechan...

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Autores principales:	Shelly Reuven, Mieka Rinsky, Vera Brekhman, Assaf Malik, Oren Levy, Tamar Lotan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95754d0498194f5b9ee8d3d6ad2becf9
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spelling	oai:doaj.org-article:95754d0498194f5b9ee8d3d6ad2becf92021-12-02T18:47:04ZCellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis10.1038/s41598-021-95033-32045-2322https://doaj.org/article/95754d0498194f5b9ee8d3d6ad2becf92021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95033-3https://doaj.org/toc/2045-2322Abstract In cnidarians, long-term ecological success relies on sexual reproduction. The sea anemone Nematostella vectensis, which has emerged as an important model organism for developmental studies, can be induced for spawning by temperature elevation and light exposure. To uncover molecular mechanisms and pathways underlying spawning, we characterized the transcriptome of Nematostella females before and during spawning induction. We identified an array of processes involving numerous receptors, circadian clock components, cytoskeleton, and extracellular transcripts that are upregulated upon spawning induction. Concurrently, processes related to the cell cycle, fatty acid metabolism, and other housekeeping functions are downregulated. Real-time qPCR revealed that light exposure has a minor effect on expression levels of most examined transcripts, implying that temperature change is a stronger inducer for spawning in Nematostella. Our findings reveal the potential mechanisms that may enable the mesenteries to serve as a gonad-like tissue for the developing oocytes and expand our understanding of sexual reproduction in cnidarians.Shelly ReuvenMieka RinskyVera BrekhmanAssaf MalikOren LevyTamar LotanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shelly Reuven Mieka Rinsky Vera Brekhman Assaf Malik Oren Levy Tamar Lotan Cellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis
description	Abstract In cnidarians, long-term ecological success relies on sexual reproduction. The sea anemone Nematostella vectensis, which has emerged as an important model organism for developmental studies, can be induced for spawning by temperature elevation and light exposure. To uncover molecular mechanisms and pathways underlying spawning, we characterized the transcriptome of Nematostella females before and during spawning induction. We identified an array of processes involving numerous receptors, circadian clock components, cytoskeleton, and extracellular transcripts that are upregulated upon spawning induction. Concurrently, processes related to the cell cycle, fatty acid metabolism, and other housekeeping functions are downregulated. Real-time qPCR revealed that light exposure has a minor effect on expression levels of most examined transcripts, implying that temperature change is a stronger inducer for spawning in Nematostella. Our findings reveal the potential mechanisms that may enable the mesenteries to serve as a gonad-like tissue for the developing oocytes and expand our understanding of sexual reproduction in cnidarians.
format	article
author	Shelly Reuven Mieka Rinsky Vera Brekhman Assaf Malik Oren Levy Tamar Lotan
author_facet	Shelly Reuven Mieka Rinsky Vera Brekhman Assaf Malik Oren Levy Tamar Lotan
author_sort	Shelly Reuven
title	Cellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis
title_short	Cellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis
title_full	Cellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis
title_fullStr	Cellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis
title_full_unstemmed	Cellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis
title_sort	cellular pathways during spawning induction in the starlet sea anemone nematostella vectensis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/95754d0498194f5b9ee8d3d6ad2becf9
work_keys_str_mv	AT shellyreuven cellularpathwaysduringspawninginductioninthestarletseaanemonenematostellavectensis AT miekarinsky cellularpathwaysduringspawninginductioninthestarletseaanemonenematostellavectensis AT verabrekhman cellularpathwaysduringspawninginductioninthestarletseaanemonenematostellavectensis AT assafmalik cellularpathwaysduringspawninginductioninthestarletseaanemonenematostellavectensis AT orenlevy cellularpathwaysduringspawninginductioninthestarletseaanemonenematostellavectensis AT tamarlotan cellularpathwaysduringspawninginductioninthestarletseaanemonenematostellavectensis
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Cellular pathways during spawning induction in the starlet sea anemone Nematostella vectensis

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