Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing

Urinary stones and urinary tract infection (UTI) are the most common diseases in urology and they are characterized by high incidence and high recurrence rate in China. Previous studies have shown that urinary stones are closely associated with gut or urine microbiota. Calcium oxalate stones are the...

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Autores principales:	Chen Shen, Qianhui Zhu, Fan Dong, Wei Wang, Bo Fan, Kexin Li, Jun Chen, Songnian Hu, Zilong He, Xiancheng Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	urinary tract infection 16S rDNA sequencing differentially abundant taxa calcium oxalate stones urinary stone microbiota Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9576b2ad9bb9401fb6ce9a8a353c977c
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spelling	oai:doaj.org-article:9576b2ad9bb9401fb6ce9a8a353c977c2021-11-17T05:46:32ZIdentifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing2235-298810.3389/fcimb.2021.723781https://doaj.org/article/9576b2ad9bb9401fb6ce9a8a353c977c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.723781/fullhttps://doaj.org/toc/2235-2988Urinary stones and urinary tract infection (UTI) are the most common diseases in urology and they are characterized by high incidence and high recurrence rate in China. Previous studies have shown that urinary stones are closely associated with gut or urine microbiota. Calcium oxalate stones are the most common type of urinary stones. However, the profile of urinary tract microorganisms of calcium oxalate stones with UTI is not clear. In this research, we firstly found two novel clusters in patients with calcium oxalate stones (OA) that were associated with the WBC/HP (white blood cells per high-power field) level in urine. Two clusters in the OA group (OA1 and OA2) were distinguished by the key microbiota Firmicutes and Enterobacteriaceae. We found that Enterobacteriaceae enriched in OA1 cluster was positively correlated with several infection-related pathways and negatively correlated with a few antibiotics-related pathways. Meantime, some probiotics with higher abundance in OA2 cluster such as Bifidobacterium were positively correlated with antibiotics-related pathways, and some common pathogens with higher abundance in OA2 cluster such as Enterococcus were positively correlated with infection-related pathways. Therefore, we speculated that as a sub-type of OA disease, OA1 was caused by Enterobacteriaceae and the lack of probiotics compared with OA2 cluster. Moreover, we also sequenced urine samples of healthy individuals (CK), patients with UTI (I), patients with uric acid stones (UA), and patients with infection stones (IS). We identified the differentially abundant taxa among all groups. We hope the findings will be helpful for clinical treatment and diagnosis of urinary stones.Chen ShenQianhui ZhuQianhui ZhuFan DongWei WangBo FanKexin LiKexin LiJun ChenSongnian HuSongnian HuZilong HeZilong HeXiancheng LiFrontiers Media S.A.articleurinary tract infection16S rDNA sequencingdifferentially abundant taxacalcium oxalate stonesurinary stonemicrobiotaMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	urinary tract infection 16S rDNA sequencing differentially abundant taxa calcium oxalate stones urinary stone microbiota Microbiology QR1-502
spellingShingle	urinary tract infection 16S rDNA sequencing differentially abundant taxa calcium oxalate stones urinary stone microbiota Microbiology QR1-502 Chen Shen Qianhui Zhu Qianhui Zhu Fan Dong Wei Wang Bo Fan Kexin Li Kexin Li Jun Chen Songnian Hu Songnian Hu Zilong He Zilong He Xiancheng Li Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing
description	Urinary stones and urinary tract infection (UTI) are the most common diseases in urology and they are characterized by high incidence and high recurrence rate in China. Previous studies have shown that urinary stones are closely associated with gut or urine microbiota. Calcium oxalate stones are the most common type of urinary stones. However, the profile of urinary tract microorganisms of calcium oxalate stones with UTI is not clear. In this research, we firstly found two novel clusters in patients with calcium oxalate stones (OA) that were associated with the WBC/HP (white blood cells per high-power field) level in urine. Two clusters in the OA group (OA1 and OA2) were distinguished by the key microbiota Firmicutes and Enterobacteriaceae. We found that Enterobacteriaceae enriched in OA1 cluster was positively correlated with several infection-related pathways and negatively correlated with a few antibiotics-related pathways. Meantime, some probiotics with higher abundance in OA2 cluster such as Bifidobacterium were positively correlated with antibiotics-related pathways, and some common pathogens with higher abundance in OA2 cluster such as Enterococcus were positively correlated with infection-related pathways. Therefore, we speculated that as a sub-type of OA disease, OA1 was caused by Enterobacteriaceae and the lack of probiotics compared with OA2 cluster. Moreover, we also sequenced urine samples of healthy individuals (CK), patients with UTI (I), patients with uric acid stones (UA), and patients with infection stones (IS). We identified the differentially abundant taxa among all groups. We hope the findings will be helpful for clinical treatment and diagnosis of urinary stones.
format	article
author	Chen Shen Qianhui Zhu Qianhui Zhu Fan Dong Wei Wang Bo Fan Kexin Li Kexin Li Jun Chen Songnian Hu Songnian Hu Zilong He Zilong He Xiancheng Li
author_facet	Chen Shen Qianhui Zhu Qianhui Zhu Fan Dong Wei Wang Bo Fan Kexin Li Kexin Li Jun Chen Songnian Hu Songnian Hu Zilong He Zilong He Xiancheng Li
author_sort	Chen Shen
title	Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing
title_short	Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing
title_full	Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing
title_fullStr	Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing
title_full_unstemmed	Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing
title_sort	identifying two novel clusters in calcium oxalate stones with urinary tract infection using 16s rdna sequencing
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/9576b2ad9bb9401fb6ce9a8a353c977c
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Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing

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