Proteomic analysis of phytase transgenic and non-transgenic maize seeds

Abstract Proteomics has become a powerful technique for investigating unintended effects in genetically modified crops. In this study, we performed a comparative proteomics of the seeds of phytase-transgenic (PT) and non-transgenic (NT) maize using 2-DE and iTRAQ techniques. A total of 148 different...

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Autores principales:	Yanhua Tan, Zheng Tong, Qian Yang, Yong Sun, Xiang Jin, Cunzhi Peng, Anping Guo, Xuchu Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9578f39b779d457ba053bafb52016b1b
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spelling	oai:doaj.org-article:9578f39b779d457ba053bafb52016b1b2021-12-02T11:53:13ZProteomic analysis of phytase transgenic and non-transgenic maize seeds10.1038/s41598-017-09557-82045-2322https://doaj.org/article/9578f39b779d457ba053bafb52016b1b2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09557-8https://doaj.org/toc/2045-2322Abstract Proteomics has become a powerful technique for investigating unintended effects in genetically modified crops. In this study, we performed a comparative proteomics of the seeds of phytase-transgenic (PT) and non-transgenic (NT) maize using 2-DE and iTRAQ techniques. A total of 148 differentially expressed proteins (DEPs), including 106 down-regulated and 42 up-regulated proteins in PT, were identified. Of these proteins, 32 were identified through 2-DE and 116 were generated by iTRAQ. It is noteworthy that only three proteins could be detected via both iTRAQ and 2-DE, and most of the identified DEPs were not newly produced proteins but proteins with altered abundance. These results indicated that many DEPs could be detected in the proteome of PT maize seeds and the corresponding wild type after overexpression of the target gene, but the changes in these proteins were not substantial. Functional classification revealed many DEPs involved in posttranscriptional modifications and some ribosomal proteins and heat-shock proteins that may generate adaptive effects in response to the insertion of exogenous genes. Protein-protein interaction analysis demonstrated that the detected interacting proteins were mainly ribosomal proteins and heat-shock proteins. Our data provided new information on such unintended effects through a proteomic analysis of maize seeds.Yanhua TanZheng TongQian YangYong SunXiang JinCunzhi PengAnping GuoXuchu WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanhua Tan Zheng Tong Qian Yang Yong Sun Xiang Jin Cunzhi Peng Anping Guo Xuchu Wang Proteomic analysis of phytase transgenic and non-transgenic maize seeds
description	Abstract Proteomics has become a powerful technique for investigating unintended effects in genetically modified crops. In this study, we performed a comparative proteomics of the seeds of phytase-transgenic (PT) and non-transgenic (NT) maize using 2-DE and iTRAQ techniques. A total of 148 differentially expressed proteins (DEPs), including 106 down-regulated and 42 up-regulated proteins in PT, were identified. Of these proteins, 32 were identified through 2-DE and 116 were generated by iTRAQ. It is noteworthy that only three proteins could be detected via both iTRAQ and 2-DE, and most of the identified DEPs were not newly produced proteins but proteins with altered abundance. These results indicated that many DEPs could be detected in the proteome of PT maize seeds and the corresponding wild type after overexpression of the target gene, but the changes in these proteins were not substantial. Functional classification revealed many DEPs involved in posttranscriptional modifications and some ribosomal proteins and heat-shock proteins that may generate adaptive effects in response to the insertion of exogenous genes. Protein-protein interaction analysis demonstrated that the detected interacting proteins were mainly ribosomal proteins and heat-shock proteins. Our data provided new information on such unintended effects through a proteomic analysis of maize seeds.
format	article
author	Yanhua Tan Zheng Tong Qian Yang Yong Sun Xiang Jin Cunzhi Peng Anping Guo Xuchu Wang
author_facet	Yanhua Tan Zheng Tong Qian Yang Yong Sun Xiang Jin Cunzhi Peng Anping Guo Xuchu Wang
author_sort	Yanhua Tan
title	Proteomic analysis of phytase transgenic and non-transgenic maize seeds
title_short	Proteomic analysis of phytase transgenic and non-transgenic maize seeds
title_full	Proteomic analysis of phytase transgenic and non-transgenic maize seeds
title_fullStr	Proteomic analysis of phytase transgenic and non-transgenic maize seeds
title_full_unstemmed	Proteomic analysis of phytase transgenic and non-transgenic maize seeds
title_sort	proteomic analysis of phytase transgenic and non-transgenic maize seeds
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/9578f39b779d457ba053bafb52016b1b
work_keys_str_mv	AT yanhuatan proteomicanalysisofphytasetransgenicandnontransgenicmaizeseeds AT zhengtong proteomicanalysisofphytasetransgenicandnontransgenicmaizeseeds AT qianyang proteomicanalysisofphytasetransgenicandnontransgenicmaizeseeds AT yongsun proteomicanalysisofphytasetransgenicandnontransgenicmaizeseeds AT xiangjin proteomicanalysisofphytasetransgenicandnontransgenicmaizeseeds AT cunzhipeng proteomicanalysisofphytasetransgenicandnontransgenicmaizeseeds AT anpingguo proteomicanalysisofphytasetransgenicandnontransgenicmaizeseeds AT xuchuwang proteomicanalysisofphytasetransgenicandnontransgenicmaizeseeds
_version_	1718394879410700288

Proteomic analysis of phytase transgenic and non-transgenic maize seeds

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