Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. pauca strain CoDiRO

Abstract Xylella fastidiosa subsp. pauca strain CoDiRO, a pathogen responsible for Olive Quick Decline Syndrome (OQDS), is strongly threatening the agricultural-based economy of South Italy and making its typical landscape collapse. The bacteria can also infect more than other twenty woody or shrub...

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Autores principales:	Maria Serena Chiriacò, Andrea Luvisi, Elisabetta Primiceri, Erika Sabella, Luigi De Bellis, Giuseppe Maruccio
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/957d42fe6f524c3d9386c783a160791e
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spelling	oai:doaj.org-article:957d42fe6f524c3d9386c783a160791e2021-12-02T16:07:50ZDevelopment of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. pauca strain CoDiRO10.1038/s41598-018-25747-42045-2322https://doaj.org/article/957d42fe6f524c3d9386c783a160791e2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25747-4https://doaj.org/toc/2045-2322Abstract Xylella fastidiosa subsp. pauca strain CoDiRO, a pathogen responsible for Olive Quick Decline Syndrome (OQDS), is strongly threatening the agricultural-based economy of South Italy and making its typical landscape collapse. The bacteria can also infect more than other twenty woody or shrub species and quarantine programs are carried out in Italy. Since symptoms of OQDS like leaf scorching and wilting of canopy may appear several months after infection and some hosts are asymptomatic, a tool for the rapid and early screening of plants is desirable, in order to plan a sudden control strategy and apply programs for pest management. X. fastidiosa detection is usually performed by ELISA and PCR methods. In this work, the two standard methods are compared with an innovative on-chip detection strategy for X. fastidiosa assay from leaves samples, based on an electrochemical transduction method. The realized lab-on-chip includes also a microfluidic module and its performances are competitive with conventional diagnostic methods in terms of reliability, but with further advantages of portability, low-costs and ease of use. Thus, the proposed technology has the potential to provide a useful assay method for large-scale monitoring programs.Maria Serena ChiriacòAndrea LuvisiElisabetta PrimiceriErika SabellaLuigi De BellisGiuseppe MaruccioNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-8 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maria Serena Chiriacò Andrea Luvisi Elisabetta Primiceri Erika Sabella Luigi De Bellis Giuseppe Maruccio Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. pauca strain CoDiRO
description	Abstract Xylella fastidiosa subsp. pauca strain CoDiRO, a pathogen responsible for Olive Quick Decline Syndrome (OQDS), is strongly threatening the agricultural-based economy of South Italy and making its typical landscape collapse. The bacteria can also infect more than other twenty woody or shrub species and quarantine programs are carried out in Italy. Since symptoms of OQDS like leaf scorching and wilting of canopy may appear several months after infection and some hosts are asymptomatic, a tool for the rapid and early screening of plants is desirable, in order to plan a sudden control strategy and apply programs for pest management. X. fastidiosa detection is usually performed by ELISA and PCR methods. In this work, the two standard methods are compared with an innovative on-chip detection strategy for X. fastidiosa assay from leaves samples, based on an electrochemical transduction method. The realized lab-on-chip includes also a microfluidic module and its performances are competitive with conventional diagnostic methods in terms of reliability, but with further advantages of portability, low-costs and ease of use. Thus, the proposed technology has the potential to provide a useful assay method for large-scale monitoring programs.
format	article
author	Maria Serena Chiriacò Andrea Luvisi Elisabetta Primiceri Erika Sabella Luigi De Bellis Giuseppe Maruccio
author_facet	Maria Serena Chiriacò Andrea Luvisi Elisabetta Primiceri Erika Sabella Luigi De Bellis Giuseppe Maruccio
author_sort	Maria Serena Chiriacò
title	Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. pauca strain CoDiRO
title_short	Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. pauca strain CoDiRO
title_full	Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. pauca strain CoDiRO
title_fullStr	Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. pauca strain CoDiRO
title_full_unstemmed	Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. pauca strain CoDiRO
title_sort	development of a lab-on-a-chip method for rapid assay of xylella fastidiosa subsp. pauca strain codiro
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/957d42fe6f524c3d9386c783a160791e
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Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. pauca strain CoDiRO

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