Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy

Shih-Heng Tseng,1,2 Min-Yuan Chou,2 I-Ming Chu1 1Department of Chemical Engineering, National Tsing Hua University, 2Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan Abstract: We have developed a theranostic nanoparticle, ie, cet-PEG...

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Autores principales:	Tseng SH, Chou MY, Chu IM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/957e22a807544480b2882f0db0d86901
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spelling	oai:doaj.org-article:957e22a807544480b2882f0db0d869012021-12-02T07:46:33ZCetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy1178-2013https://doaj.org/article/957e22a807544480b2882f0db0d869012015-05-01T00:00:00Zhttp://www.dovepress.com/cetuximab-conjugated-iron-oxide-nanoparticles-for-cancer-imaging-and-t-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Shih-Heng Tseng,1,2 Min-Yuan Chou,2 I-Ming Chu1 1Department of Chemical Engineering, National Tsing Hua University, 2Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan Abstract: We have developed a theranostic nanoparticle, ie, cet-PEG-dexSPIONs, by conjugation of the anti-epidermal growth factor receptor (EGFR) monoclonal antibody, cetuximab, to dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) via periodate oxidation. Approximately 31 antibody molecules were conjugated to each nanoparticle. Cet-PEG-dexSPIONs specifically bind to EGFR-expressing tumor cells and enhance image contrast on magnetic resonance imaging. Cet-PEG-dexSPION-treated A431 cells showed significant inhibition of epidermal growth factor-induced EGFR phosphorylation and enhancement of EGFR internalization and degradation. In addition, a significant increase in apoptosis was detected in EGFR-overexpressing cell lines, A431 and 32D/EGFR, after 24 hours of incubation at 37°C with cet-PEG-dexSPIONs compared with cetuximab alone. The antibody-dependent cell-mediated cytotoxicity of cetuximab was observed in cet-PEG-dexSPIONs. The results demonstrated that cet-PEG-dexSPIONs retained the therapeutic effect of cetuximab in addition to having the ability to target and image EGFR-expressing tumors. Cet-PEG-dexSPIONs represent a promising targeted magnetic probe for early detection and treatment of EGFR-expressing tumor cells. Keywords: epidermal growth factor receptor, cetuximab, superparamagnetic iron oxide nanoparticle, magnetic resonance imaging, sodium periodate, polyethylene glycolTseng SHChou MYChu IMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 3663-3685 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Tseng SH Chou MY Chu IM Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy
description	Shih-Heng Tseng,1,2 Min-Yuan Chou,2 I-Ming Chu1 1Department of Chemical Engineering, National Tsing Hua University, 2Biomedical Technology and Device Research Laboratories, Industrial Technology Research Institute, Hsinchu, Taiwan Abstract: We have developed a theranostic nanoparticle, ie, cet-PEG-dexSPIONs, by conjugation of the anti-epidermal growth factor receptor (EGFR) monoclonal antibody, cetuximab, to dextran-coated superparamagnetic iron oxide nanoparticles (SPIONs) via periodate oxidation. Approximately 31 antibody molecules were conjugated to each nanoparticle. Cet-PEG-dexSPIONs specifically bind to EGFR-expressing tumor cells and enhance image contrast on magnetic resonance imaging. Cet-PEG-dexSPION-treated A431 cells showed significant inhibition of epidermal growth factor-induced EGFR phosphorylation and enhancement of EGFR internalization and degradation. In addition, a significant increase in apoptosis was detected in EGFR-overexpressing cell lines, A431 and 32D/EGFR, after 24 hours of incubation at 37°C with cet-PEG-dexSPIONs compared with cetuximab alone. The antibody-dependent cell-mediated cytotoxicity of cetuximab was observed in cet-PEG-dexSPIONs. The results demonstrated that cet-PEG-dexSPIONs retained the therapeutic effect of cetuximab in addition to having the ability to target and image EGFR-expressing tumors. Cet-PEG-dexSPIONs represent a promising targeted magnetic probe for early detection and treatment of EGFR-expressing tumor cells. Keywords: epidermal growth factor receptor, cetuximab, superparamagnetic iron oxide nanoparticle, magnetic resonance imaging, sodium periodate, polyethylene glycol
format	article
author	Tseng SH Chou MY Chu IM
author_facet	Tseng SH Chou MY Chu IM
author_sort	Tseng SH
title	Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy
title_short	Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy
title_full	Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy
title_fullStr	Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy
title_full_unstemmed	Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy
title_sort	cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/957e22a807544480b2882f0db0d86901
work_keys_str_mv	AT tsengsh cetuximabconjugatedironoxidenanoparticlesforcancerimagingandtherapy AT choumy cetuximabconjugatedironoxidenanoparticlesforcancerimagingandtherapy AT chuim cetuximabconjugatedironoxidenanoparticlesforcancerimagingandtherapy
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Cetuximab-conjugated iron oxide nanoparticles for cancer imaging and therapy

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