Ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology

Guangcheng Zhang,1 Mingjun Nie,2 Thomas J Webster,3 Qing Zhang,2 Weimin Fan11Department of Orthopedics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 2Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang, People...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhang G, Nie M, Webster TJ, Zhang Q, Fan W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Sox9 chondrogenesis gene enhanced tissue engineering transfection chondroid Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/9584f54b62594168a5ccbfcbad82308a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:9584f54b62594168a5ccbfcbad82308a
record_format	dspace
spelling	oai:doaj.org-article:9584f54b62594168a5ccbfcbad82308a2021-12-02T03:23:40ZEctopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology1178-2013https://doaj.org/article/9584f54b62594168a5ccbfcbad82308a2019-07-01T00:00:00Zhttps://www.dovepress.com/ectopic-chondrogenesis-of-nude-mouse-induced-by-nano-gene-delivery-enh-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Guangcheng Zhang,1 Mingjun Nie,2 Thomas J Webster,3 Qing Zhang,2 Weimin Fan11Department of Orthopedics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 2Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang, People’s Republic of China; 3Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USABackground: Many techniques and methods have been used clinically to relieve pain from cartilage repair, but the long-term effect is still unsatisfactory.Purpose: The objective of this study was to form an artificial chondroid tissue gene enhanced tissue engineering system to repair cartilage defects via nanosized liposomes.Methods: Cationic nanosized liposomes were prepared and characterized using transmission electron microscope (TEM) and dynamic laser light scattering (DLS). The rat mesenchymal stem cells (rMSCs) were isolated, cultivated, and induced by SRY (Sex-Determining Region Y)-Box 9 (Sox9) via cationic nanosized liposomes. The induced rMSCs were mixed with a thermo-sensitive chitosan hydrogel and subcutaneously injected into the nude mice. Finally, the newly-formed chondroid tissue obtained in the injection parts, and the transparent parts were detected by HE, collagen II, and safranin O.Results: It was found that the presently prepared cationic nanosized liposomes had the diameter of 85.76±3.48 nm and the zeta potential of 15.76±2.1 mV. The isolated rMSCs proliferation was fibroblast-like, with a cultivated confluence of 90% confluence in 5–8 days, and stained positive for CD29 and CD44 while negative for CD34 and CD45. After transfection with cationic nanosized liposomes, we observed changes of cellular morphology and a higher expression of SOX9 compared with control groups, which indicated that rMSCs could differentiate into chondrocyte in vitro. By mixing transfected rMSCs with the thermo-sensitive hydrogel of chitosan in nude mice, chondroid tissue was successfully obtained, demonstrating that rMSCs can differentiate into chondrogenic cells in vivo. Conclusion: This study explored new ways to improve the quality of tissue engineered cartilage, thus accelerating clinical transformation and reducing patient pain.Keywords: Sox9, chondrogenesis, gene enhanced tissue engineering, transfection, chondroidZhang GNie MWebster TJZhang QFan WDove Medical PressarticleSox9chondrogenesisgene enhanced tissue engineeringtransfectionchondroidMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 4755-4765 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Sox9 chondrogenesis gene enhanced tissue engineering transfection chondroid Medicine (General) R5-920
spellingShingle	Sox9 chondrogenesis gene enhanced tissue engineering transfection chondroid Medicine (General) R5-920 Zhang G Nie M Webster TJ Zhang Q Fan W Ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology
description	Guangcheng Zhang,1 Mingjun Nie,2 Thomas J Webster,3 Qing Zhang,2 Weimin Fan11Department of Orthopedics, the First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of China; 2Department of Orthopedics, Affiliated Hospital of Jiangsu University, Zhenjiang, People’s Republic of China; 3Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USABackground: Many techniques and methods have been used clinically to relieve pain from cartilage repair, but the long-term effect is still unsatisfactory.Purpose: The objective of this study was to form an artificial chondroid tissue gene enhanced tissue engineering system to repair cartilage defects via nanosized liposomes.Methods: Cationic nanosized liposomes were prepared and characterized using transmission electron microscope (TEM) and dynamic laser light scattering (DLS). The rat mesenchymal stem cells (rMSCs) were isolated, cultivated, and induced by SRY (Sex-Determining Region Y)-Box 9 (Sox9) via cationic nanosized liposomes. The induced rMSCs were mixed with a thermo-sensitive chitosan hydrogel and subcutaneously injected into the nude mice. Finally, the newly-formed chondroid tissue obtained in the injection parts, and the transparent parts were detected by HE, collagen II, and safranin O.Results: It was found that the presently prepared cationic nanosized liposomes had the diameter of 85.76±3.48 nm and the zeta potential of 15.76±2.1 mV. The isolated rMSCs proliferation was fibroblast-like, with a cultivated confluence of 90% confluence in 5–8 days, and stained positive for CD29 and CD44 while negative for CD34 and CD45. After transfection with cationic nanosized liposomes, we observed changes of cellular morphology and a higher expression of SOX9 compared with control groups, which indicated that rMSCs could differentiate into chondrocyte in vitro. By mixing transfected rMSCs with the thermo-sensitive hydrogel of chitosan in nude mice, chondroid tissue was successfully obtained, demonstrating that rMSCs can differentiate into chondrogenic cells in vivo. Conclusion: This study explored new ways to improve the quality of tissue engineered cartilage, thus accelerating clinical transformation and reducing patient pain.Keywords: Sox9, chondrogenesis, gene enhanced tissue engineering, transfection, chondroid
format	article
author	Zhang G Nie M Webster TJ Zhang Q Fan W
author_facet	Zhang G Nie M Webster TJ Zhang Q Fan W
author_sort	Zhang G
title	Ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology
title_short	Ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology
title_full	Ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology
title_fullStr	Ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology
title_full_unstemmed	Ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology
title_sort	ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/9584f54b62594168a5ccbfcbad82308a
work_keys_str_mv	AT zhangg ectopicchondrogenesisofnudemouseinducedbynanogenedeliveryenhancedtissueengineeringtechnology AT niem ectopicchondrogenesisofnudemouseinducedbynanogenedeliveryenhancedtissueengineeringtechnology AT webstertj ectopicchondrogenesisofnudemouseinducedbynanogenedeliveryenhancedtissueengineeringtechnology AT zhangq ectopicchondrogenesisofnudemouseinducedbynanogenedeliveryenhancedtissueengineeringtechnology AT fanw ectopicchondrogenesisofnudemouseinducedbynanogenedeliveryenhancedtissueengineeringtechnology
_version_	1718401809153785856

Ectopic chondrogenesis of nude mouse induced by nano gene delivery enhanced tissue engineering technology

Ejemplares similares