Maternal High-Fat Diet Programs Offspring Liver Steatosis in a Sexually Dimorphic Manner in Association with Changes in Gut Microbial Ecology in Mice

Abstract The contributions of maternal diet and obesity in shaping offspring microbiome remain unclear. Here we employed a mouse model of maternal diet-induced obesity via high-fat diet feeding (HFD, 45% fat calories) for 12 wk prior to conception on offspring gut microbial ecology. Male and female...

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Autores principales:	Umesh D. Wankhade, Ying Zhong, Ping Kang, Maria Alfaro, Sree V. Chintapalli, Brian D. Piccolo, Kelly E. Mercer, Aline Andres, Keshari M. Thakali, Kartik Shankar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Maternal Diet Female Offspring Microbiome Male Offspring Serum Bile Acid Concentrations Medicine R Science Q
Acceso en línea:	https://doaj.org/article/959da76f8935497394ad32e4ff3c4811
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spelling	oai:doaj.org-article:959da76f8935497394ad32e4ff3c48112021-12-02T15:08:13ZMaternal High-Fat Diet Programs Offspring Liver Steatosis in a Sexually Dimorphic Manner in Association with Changes in Gut Microbial Ecology in Mice10.1038/s41598-018-34453-02045-2322https://doaj.org/article/959da76f8935497394ad32e4ff3c48112018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34453-0https://doaj.org/toc/2045-2322Abstract The contributions of maternal diet and obesity in shaping offspring microbiome remain unclear. Here we employed a mouse model of maternal diet-induced obesity via high-fat diet feeding (HFD, 45% fat calories) for 12 wk prior to conception on offspring gut microbial ecology. Male and female offspring were provided access to control or HFD from weaning until 17 wk of age. Maternal HFD-associated programming was sexually dimorphic, with male offspring from HFD dams showing hyper-responsive weight gain to postnatal HFD. Likewise, microbiome analysis of offspring cecal contents showed differences in α-diversity, β-diversity and higher Firmicutes in male compared to female mice. Weight gain in offspring was significantly associated with abundance of Lachnospiraceae and Clostridiaceae families and Adlercreutzia, Coprococcus and Lactococcus genera. Sex differences in metagenomic pathways relating to lipid metabolism, bile acid biosynthesis and immune response were also observed. HFD-fed male offspring from HFD dams also showed worse hepatic pathology, increased pro-inflammatory cytokines, altered expression of bile acid regulators (Cyp7a1, Cyp8b1 and Cyp39a1) and serum bile acid concentrations. These findings suggest that maternal HFD alters gut microbiota composition and weight gain of offspring in a sexually dimorphic manner, coincident with fatty liver and a pro-inflammatory state in male offspring.Umesh D. WankhadeYing ZhongPing KangMaria AlfaroSree V. ChintapalliBrian D. PiccoloKelly E. MercerAline AndresKeshari M. ThakaliKartik ShankarNature PortfolioarticleMaternal DietFemale OffspringMicrobiomeMale OffspringSerum Bile Acid ConcentrationsMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Maternal Diet Female Offspring Microbiome Male Offspring Serum Bile Acid Concentrations Medicine R Science Q
spellingShingle	Maternal Diet Female Offspring Microbiome Male Offspring Serum Bile Acid Concentrations Medicine R Science Q Umesh D. Wankhade Ying Zhong Ping Kang Maria Alfaro Sree V. Chintapalli Brian D. Piccolo Kelly E. Mercer Aline Andres Keshari M. Thakali Kartik Shankar Maternal High-Fat Diet Programs Offspring Liver Steatosis in a Sexually Dimorphic Manner in Association with Changes in Gut Microbial Ecology in Mice
description	Abstract The contributions of maternal diet and obesity in shaping offspring microbiome remain unclear. Here we employed a mouse model of maternal diet-induced obesity via high-fat diet feeding (HFD, 45% fat calories) for 12 wk prior to conception on offspring gut microbial ecology. Male and female offspring were provided access to control or HFD from weaning until 17 wk of age. Maternal HFD-associated programming was sexually dimorphic, with male offspring from HFD dams showing hyper-responsive weight gain to postnatal HFD. Likewise, microbiome analysis of offspring cecal contents showed differences in α-diversity, β-diversity and higher Firmicutes in male compared to female mice. Weight gain in offspring was significantly associated with abundance of Lachnospiraceae and Clostridiaceae families and Adlercreutzia, Coprococcus and Lactococcus genera. Sex differences in metagenomic pathways relating to lipid metabolism, bile acid biosynthesis and immune response were also observed. HFD-fed male offspring from HFD dams also showed worse hepatic pathology, increased pro-inflammatory cytokines, altered expression of bile acid regulators (Cyp7a1, Cyp8b1 and Cyp39a1) and serum bile acid concentrations. These findings suggest that maternal HFD alters gut microbiota composition and weight gain of offspring in a sexually dimorphic manner, coincident with fatty liver and a pro-inflammatory state in male offspring.
format	article
author	Umesh D. Wankhade Ying Zhong Ping Kang Maria Alfaro Sree V. Chintapalli Brian D. Piccolo Kelly E. Mercer Aline Andres Keshari M. Thakali Kartik Shankar
author_facet	Umesh D. Wankhade Ying Zhong Ping Kang Maria Alfaro Sree V. Chintapalli Brian D. Piccolo Kelly E. Mercer Aline Andres Keshari M. Thakali Kartik Shankar
author_sort	Umesh D. Wankhade
title	Maternal High-Fat Diet Programs Offspring Liver Steatosis in a Sexually Dimorphic Manner in Association with Changes in Gut Microbial Ecology in Mice
title_short	Maternal High-Fat Diet Programs Offspring Liver Steatosis in a Sexually Dimorphic Manner in Association with Changes in Gut Microbial Ecology in Mice
title_full	Maternal High-Fat Diet Programs Offspring Liver Steatosis in a Sexually Dimorphic Manner in Association with Changes in Gut Microbial Ecology in Mice
title_fullStr	Maternal High-Fat Diet Programs Offspring Liver Steatosis in a Sexually Dimorphic Manner in Association with Changes in Gut Microbial Ecology in Mice
title_full_unstemmed	Maternal High-Fat Diet Programs Offspring Liver Steatosis in a Sexually Dimorphic Manner in Association with Changes in Gut Microbial Ecology in Mice
title_sort	maternal high-fat diet programs offspring liver steatosis in a sexually dimorphic manner in association with changes in gut microbial ecology in mice
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/959da76f8935497394ad32e4ff3c4811
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Maternal High-Fat Diet Programs Offspring Liver Steatosis in a Sexually Dimorphic Manner in Association with Changes in Gut Microbial Ecology in Mice

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