ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons

Abstract Local protein synthesis occurs in axons and dendrites of neurons, enabling fast and spatially restricted responses to a dynamically changing extracellular environment. Prior to local translation, mRNA that is to be translated is packed into ribonucleoprotein particles (RNPs) where RNA bindi...

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Autores principales:	Anna S. Urbanska, Aleksandra Janusz-Kaminska, Katarzyna Switon, Alicia L. Hawthorne, Malgorzata Perycz, Malgorzata Urbanska, Gary J. Bassell, Jacek Jaworski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/959f5bf9d0a84195b62739a8b8eee718
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spelling	oai:doaj.org-article:959f5bf9d0a84195b62739a8b8eee7182021-12-02T12:32:46ZZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons10.1038/s41598-017-01963-22045-2322https://doaj.org/article/959f5bf9d0a84195b62739a8b8eee7182017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01963-2https://doaj.org/toc/2045-2322Abstract Local protein synthesis occurs in axons and dendrites of neurons, enabling fast and spatially restricted responses to a dynamically changing extracellular environment. Prior to local translation, mRNA that is to be translated is packed into ribonucleoprotein particles (RNPs) where RNA binding proteins ensure mRNA silencing and provide a link to molecular motors. ZBP1 is a component of RNP transport particles and is known for its role in the local translation of β-actin mRNA. Its binding to mRNA is regulated by tyrosine 396 phosphorylation, and this particular modification was shown to be vital for axonal growth and dendritic branching. Recently, additional phosphorylation of ZBP1 at serine 181 (Ser181) was described in non-neuronal cells. In the present study, we found that ZBP1 is also phosphorylated at Ser181 in neurons in a mammalian/mechanistic target of rapamycin complex 2-, Src kinase-, and mRNA binding-dependent manner. Furthermore, Ser181 ZBP1 phosphorylation was essential for the proper dendritic branching of hippocampal neurons that were cultured in vitro and for the proper ZBP1 dendritic distribution and motility.Anna S. UrbanskaAleksandra Janusz-KaminskaKatarzyna SwitonAlicia L. HawthorneMalgorzata PeryczMalgorzata UrbanskaGary J. BassellJacek JaworskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna S. Urbanska Aleksandra Janusz-Kaminska Katarzyna Switon Alicia L. Hawthorne Malgorzata Perycz Malgorzata Urbanska Gary J. Bassell Jacek Jaworski ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons
description	Abstract Local protein synthesis occurs in axons and dendrites of neurons, enabling fast and spatially restricted responses to a dynamically changing extracellular environment. Prior to local translation, mRNA that is to be translated is packed into ribonucleoprotein particles (RNPs) where RNA binding proteins ensure mRNA silencing and provide a link to molecular motors. ZBP1 is a component of RNP transport particles and is known for its role in the local translation of β-actin mRNA. Its binding to mRNA is regulated by tyrosine 396 phosphorylation, and this particular modification was shown to be vital for axonal growth and dendritic branching. Recently, additional phosphorylation of ZBP1 at serine 181 (Ser181) was described in non-neuronal cells. In the present study, we found that ZBP1 is also phosphorylated at Ser181 in neurons in a mammalian/mechanistic target of rapamycin complex 2-, Src kinase-, and mRNA binding-dependent manner. Furthermore, Ser181 ZBP1 phosphorylation was essential for the proper dendritic branching of hippocampal neurons that were cultured in vitro and for the proper ZBP1 dendritic distribution and motility.
format	article
author	Anna S. Urbanska Aleksandra Janusz-Kaminska Katarzyna Switon Alicia L. Hawthorne Malgorzata Perycz Malgorzata Urbanska Gary J. Bassell Jacek Jaworski
author_facet	Anna S. Urbanska Aleksandra Janusz-Kaminska Katarzyna Switon Alicia L. Hawthorne Malgorzata Perycz Malgorzata Urbanska Gary J. Bassell Jacek Jaworski
author_sort	Anna S. Urbanska
title	ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons
title_short	ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons
title_full	ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons
title_fullStr	ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons
title_full_unstemmed	ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons
title_sort	zbp1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/959f5bf9d0a84195b62739a8b8eee718
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ZBP1 phosphorylation at serine 181 regulates its dendritic transport and the development of dendritic trees of hippocampal neurons

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