High-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels.

The eri-1 gene encodes a 3' exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) ex...

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Autores principales:	Yingnan Bian, Wei Zhou, Yingchun Zhao, Xiaoping Li, Wei Geng, Ruixin Hao, Qing Yang, Weida Huang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95a3167c43d0439a89b784b58646c9e8
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spelling	oai:doaj.org-article:95a3167c43d0439a89b784b58646c9e82021-11-18T07:36:06ZHigh-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels.1932-620310.1371/journal.pone.0026466https://doaj.org/article/95a3167c43d0439a89b784b58646c9e82011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22039495/?tool=EBIhttps://doaj.org/toc/1932-6203The eri-1 gene encodes a 3' exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3' untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA.Yingnan BianWei ZhouYingchun ZhaoXiaoping LiWei GengRuixin HaoQing YangWeida HuangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26466 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yingnan Bian Wei Zhou Yingchun Zhao Xiaoping Li Wei Geng Ruixin Hao Qing Yang Weida Huang High-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels.
description	The eri-1 gene encodes a 3' exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3' untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA.
format	article
author	Yingnan Bian Wei Zhou Yingchun Zhao Xiaoping Li Wei Geng Ruixin Hao Qing Yang Weida Huang
author_facet	Yingnan Bian Wei Zhou Yingchun Zhao Xiaoping Li Wei Geng Ruixin Hao Qing Yang Weida Huang
author_sort	Yingnan Bian
title	High-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels.
title_short	High-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels.
title_full	High-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels.
title_fullStr	High-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels.
title_full_unstemmed	High-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels.
title_sort	high-dose sirnas upregulate mouse eri-1 at both transcription and posttranscription levels.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/95a3167c43d0439a89b784b58646c9e8
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High-dose siRNAs upregulate mouse Eri-1 at both transcription and posttranscription levels.

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