Differential expression of serotonin, tryptophan hydroxylase and monoamine oxidase A in the mammary gland of the Myotis velifer bat.

The mammary gland has long drawn the attention of the scientific community due to the limited knowledge of some fundamental aspects involved in the control of its function. Myotis velifer, a microchiropteran species, provides an interesting model to study some of the regulatory factors involved in t...

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Autores principales:	Cristián Vela Hinojosa, Miguel Angel León Galván, Miguel Tapia Rodríguez, Gerardo López Ortega, Marco Antonio Cerbón Cervantes, Carmen Adriana Mendoza Rodríguez, Patricia Padilla Cortés, Luis Antonio Martínez Méndez, Francisco Javier Jiménez Trejo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95a31f6a0fc34a9a961c5fbf7b160358
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spelling	oai:doaj.org-article:95a31f6a0fc34a9a961c5fbf7b1603582021-11-18T08:53:55ZDifferential expression of serotonin, tryptophan hydroxylase and monoamine oxidase A in the mammary gland of the Myotis velifer bat.1932-620310.1371/journal.pone.0075062https://doaj.org/article/95a31f6a0fc34a9a961c5fbf7b1603582013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24086437/?tool=EBIhttps://doaj.org/toc/1932-6203The mammary gland has long drawn the attention of the scientific community due to the limited knowledge of some fundamental aspects involved in the control of its function. Myotis velifer, a microchiropteran species, provides an interesting model to study some of the regulatory factors involved in the control of the mammary gland cycle. Having an asynchronous, monoestrous reproductive pattern, female M. velifer bats undergo drastic morphological changes of the breast during the reproductive cycle. Current research on non-chiropteran mammals indicates that serotonin (5-HT) plays a major role in the intraluminal volume homeostasis of the mammary gland during lactation; however, an analysis of both the expression and localization of the main components of the serotonergic system in the bat mammary gland is lacking. Thus, the objectives of the present study were: to describe the gross and histological anatomy of the mammary gland of M. velifer to establish the lactation period for this species; to analyze the distribution and expression of the main serotonergic components in the mammary tissues of these bats under the physiological conditions of lactation, involution and the resting phase; and to provide information on the involvement of 5-HT in the regulation of the physiological function of this organ. To assess the expression and localization of serotonergic components, multiple immunofluorescence, Western blot and HPLC methods were used. 5-HT and the enzyme that catalyzes its synthesis (TPH) were located in both myoepithelial and luminal epithelial cells, while the enzyme responsible for the catabolism of this neurohormone (MAO A) was found in luminal epithelial cells as well as in secreted products. We also found an increased expression of serotonergic components during lactation, indicating that elements of the serotonergic system may play an important role in lactation in this species of bat in a way similar to that of other mammal species.Cristián Vela HinojosaMiguel Angel León GalvánMiguel Tapia RodríguezGerardo López OrtegaMarco Antonio Cerbón CervantesCarmen Adriana Mendoza RodríguezPatricia Padilla CortésLuis Antonio Martínez MéndezFrancisco Javier Jiménez TrejoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e75062 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristián Vela Hinojosa Miguel Angel León Galván Miguel Tapia Rodríguez Gerardo López Ortega Marco Antonio Cerbón Cervantes Carmen Adriana Mendoza Rodríguez Patricia Padilla Cortés Luis Antonio Martínez Méndez Francisco Javier Jiménez Trejo Differential expression of serotonin, tryptophan hydroxylase and monoamine oxidase A in the mammary gland of the Myotis velifer bat.
description	The mammary gland has long drawn the attention of the scientific community due to the limited knowledge of some fundamental aspects involved in the control of its function. Myotis velifer, a microchiropteran species, provides an interesting model to study some of the regulatory factors involved in the control of the mammary gland cycle. Having an asynchronous, monoestrous reproductive pattern, female M. velifer bats undergo drastic morphological changes of the breast during the reproductive cycle. Current research on non-chiropteran mammals indicates that serotonin (5-HT) plays a major role in the intraluminal volume homeostasis of the mammary gland during lactation; however, an analysis of both the expression and localization of the main components of the serotonergic system in the bat mammary gland is lacking. Thus, the objectives of the present study were: to describe the gross and histological anatomy of the mammary gland of M. velifer to establish the lactation period for this species; to analyze the distribution and expression of the main serotonergic components in the mammary tissues of these bats under the physiological conditions of lactation, involution and the resting phase; and to provide information on the involvement of 5-HT in the regulation of the physiological function of this organ. To assess the expression and localization of serotonergic components, multiple immunofluorescence, Western blot and HPLC methods were used. 5-HT and the enzyme that catalyzes its synthesis (TPH) were located in both myoepithelial and luminal epithelial cells, while the enzyme responsible for the catabolism of this neurohormone (MAO A) was found in luminal epithelial cells as well as in secreted products. We also found an increased expression of serotonergic components during lactation, indicating that elements of the serotonergic system may play an important role in lactation in this species of bat in a way similar to that of other mammal species.
format	article
author	Cristián Vela Hinojosa Miguel Angel León Galván Miguel Tapia Rodríguez Gerardo López Ortega Marco Antonio Cerbón Cervantes Carmen Adriana Mendoza Rodríguez Patricia Padilla Cortés Luis Antonio Martínez Méndez Francisco Javier Jiménez Trejo
author_facet	Cristián Vela Hinojosa Miguel Angel León Galván Miguel Tapia Rodríguez Gerardo López Ortega Marco Antonio Cerbón Cervantes Carmen Adriana Mendoza Rodríguez Patricia Padilla Cortés Luis Antonio Martínez Méndez Francisco Javier Jiménez Trejo
author_sort	Cristián Vela Hinojosa
title	Differential expression of serotonin, tryptophan hydroxylase and monoamine oxidase A in the mammary gland of the Myotis velifer bat.
title_short	Differential expression of serotonin, tryptophan hydroxylase and monoamine oxidase A in the mammary gland of the Myotis velifer bat.
title_full	Differential expression of serotonin, tryptophan hydroxylase and monoamine oxidase A in the mammary gland of the Myotis velifer bat.
title_fullStr	Differential expression of serotonin, tryptophan hydroxylase and monoamine oxidase A in the mammary gland of the Myotis velifer bat.
title_full_unstemmed	Differential expression of serotonin, tryptophan hydroxylase and monoamine oxidase A in the mammary gland of the Myotis velifer bat.
title_sort	differential expression of serotonin, tryptophan hydroxylase and monoamine oxidase a in the mammary gland of the myotis velifer bat.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/95a31f6a0fc34a9a961c5fbf7b160358
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Differential expression of serotonin, tryptophan hydroxylase and monoamine oxidase A in the mammary gland of the Myotis velifer bat.

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