Aflatoxin Concentrations in Various Rice Varieties of Punjab, Pakistan

Background: Mycotoxins are secondary fungal metabolites that are produced in rice, corn, nuts and several other cereals. The contamination of food stuff by mycotoxins is a cause of numerous diseases throughout the world. Aflatoxins are potent mycotoxins that have chronic and acute toxicity. Obje...

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Autores principales:	Nasir Ishaque Tahir, Shabbir Hussain, Imran Kalim, Amina Asghar, Shahzada Khurram Syed, Muhammad Amin Idrees, Muhammad Ameen Ahmad
Formato:	article
Lenguaje:	EN
Publicado:	Jinnah University for Women 2021
Materias:	Aflatoxins, Branded, Nonbranded, Punjab, Pakistan, Rice. Medicine R Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/95ad604ca2664fee975abecc8c065198
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spelling	oai:doaj.org-article:95ad604ca2664fee975abecc8c0651982021-11-25T06:47:11ZAflatoxin Concentrations in Various Rice Varieties of Punjab, Pakistan10.37962/jbas.v12i1.4192305-87222521-8573https://doaj.org/article/95ad604ca2664fee975abecc8c0651982021-08-01T00:00:00Zhttp://jbas.juw.edu.pk/index.php/JBAS/article/view/419https://doaj.org/toc/2305-8722https://doaj.org/toc/2521-8573 Background: Mycotoxins are secondary fungal metabolites that are produced in rice, corn, nuts and several other cereals. The contamination of food stuff by mycotoxins is a cause of numerous diseases throughout the world. Aflatoxins are potent mycotoxins that have chronic and acute toxicity. Objectives: To evaluate the presence of aflatoxins B1, B2, G1 and G2 in branded and non-branded rice varieties available in Punjab, Pakistan. Methodology: Total 150 rice samples were collected in May 2019 from various cities (Islamabad, Rawalpindi, Gujar Khan, Jehlum, Kharian, Gujrat, Gujranwala, Lahore, Kasur, Okara, Sahiwal, Mian Channu, Khanewal, Multan, Bahawalpur and Rahim Yar Khan) of Pakistan. All the rice samples were subjected to physical testing methods, followed by extraction (using chloroform extraction method) and qualitative and quantitative detection of aflatoxin using Thin layer Chromatography (TLC) Technique and Enzyme linked Immunosorbent assay (ELISA). Results: No aflatoxins was detected in branded rice varieties which were stored in good packaging. The aflatoxin B1 (AFB1) was detected in 65% of poorly stored rice varieties, with maximum contamination of 8.92ppb. The lowest moisture 9.4% content was found in (branded samples) and 10.9% (non-branded samples) while highest moisture content 10.8% found in (branded samples) and 16% found in (non-branded samples). The 54 out 100 non-branded samples found to have more than 13% moisture contents which is alarming. Conclusion: With the implementation of effective strategies and special precautions during storage, harvesting, transportation, drying and harvesting, we can prevent the contamination of rice product with fungi and stop aflatoxin production which is a major threat to country economy. Nasir Ishaque TahirShabbir HussainImran KalimAmina AsgharShahzada Khurram SyedMuhammad Amin IdreesMuhammad Ameen AhmadJinnah University for WomenarticleAflatoxins, Branded, Nonbranded, Punjab, Pakistan, Rice.MedicineRBiology (General)QH301-705.5ENRADS Journal of Biological Research & Applied Science, Vol 12, Iss 1 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aflatoxins, Branded, Nonbranded, Punjab, Pakistan, Rice. Medicine R Biology (General) QH301-705.5
spellingShingle	Aflatoxins, Branded, Nonbranded, Punjab, Pakistan, Rice. Medicine R Biology (General) QH301-705.5 Nasir Ishaque Tahir Shabbir Hussain Imran Kalim Amina Asghar Shahzada Khurram Syed Muhammad Amin Idrees Muhammad Ameen Ahmad Aflatoxin Concentrations in Various Rice Varieties of Punjab, Pakistan
description	Background: Mycotoxins are secondary fungal metabolites that are produced in rice, corn, nuts and several other cereals. The contamination of food stuff by mycotoxins is a cause of numerous diseases throughout the world. Aflatoxins are potent mycotoxins that have chronic and acute toxicity. Objectives: To evaluate the presence of aflatoxins B1, B2, G1 and G2 in branded and non-branded rice varieties available in Punjab, Pakistan. Methodology: Total 150 rice samples were collected in May 2019 from various cities (Islamabad, Rawalpindi, Gujar Khan, Jehlum, Kharian, Gujrat, Gujranwala, Lahore, Kasur, Okara, Sahiwal, Mian Channu, Khanewal, Multan, Bahawalpur and Rahim Yar Khan) of Pakistan. All the rice samples were subjected to physical testing methods, followed by extraction (using chloroform extraction method) and qualitative and quantitative detection of aflatoxin using Thin layer Chromatography (TLC) Technique and Enzyme linked Immunosorbent assay (ELISA). Results: No aflatoxins was detected in branded rice varieties which were stored in good packaging. The aflatoxin B1 (AFB1) was detected in 65% of poorly stored rice varieties, with maximum contamination of 8.92ppb. The lowest moisture 9.4% content was found in (branded samples) and 10.9% (non-branded samples) while highest moisture content 10.8% found in (branded samples) and 16% found in (non-branded samples). The 54 out 100 non-branded samples found to have more than 13% moisture contents which is alarming. Conclusion: With the implementation of effective strategies and special precautions during storage, harvesting, transportation, drying and harvesting, we can prevent the contamination of rice product with fungi and stop aflatoxin production which is a major threat to country economy.
format	article
author	Nasir Ishaque Tahir Shabbir Hussain Imran Kalim Amina Asghar Shahzada Khurram Syed Muhammad Amin Idrees Muhammad Ameen Ahmad
author_facet	Nasir Ishaque Tahir Shabbir Hussain Imran Kalim Amina Asghar Shahzada Khurram Syed Muhammad Amin Idrees Muhammad Ameen Ahmad
author_sort	Nasir Ishaque Tahir
title	Aflatoxin Concentrations in Various Rice Varieties of Punjab, Pakistan
title_short	Aflatoxin Concentrations in Various Rice Varieties of Punjab, Pakistan
title_full	Aflatoxin Concentrations in Various Rice Varieties of Punjab, Pakistan
title_fullStr	Aflatoxin Concentrations in Various Rice Varieties of Punjab, Pakistan
title_full_unstemmed	Aflatoxin Concentrations in Various Rice Varieties of Punjab, Pakistan
title_sort	aflatoxin concentrations in various rice varieties of punjab, pakistan
publisher	Jinnah University for Women
publishDate	2021
url	https://doaj.org/article/95ad604ca2664fee975abecc8c065198
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Aflatoxin Concentrations in Various Rice Varieties of Punjab, Pakistan

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