Extended live-cell barcoding approach for multiplexed mass cytometry

Abstract Sample barcoding is essential in mass cytometry analysis, since it can eliminate potential procedural variations, enhance throughput, and allow simultaneous sample processing and acquisition. Sample pooling after prior surface staining termed live-cell barcoding is more desirable than intra...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Muharrem Muftuoglu, Li Li, Shaoheng Liang, Duncan Mak, Angelique J. Lin, Junxiang Fang, Jared K. Burks, Ken Chen, Michael Andreeff
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95adbb71ff4b407bbd8d5e2b9fd48465
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:95adbb71ff4b407bbd8d5e2b9fd48465
record_format	dspace
spelling	oai:doaj.org-article:95adbb71ff4b407bbd8d5e2b9fd484652021-12-02T17:30:40ZExtended live-cell barcoding approach for multiplexed mass cytometry10.1038/s41598-021-91816-w2045-2322https://doaj.org/article/95adbb71ff4b407bbd8d5e2b9fd484652021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91816-whttps://doaj.org/toc/2045-2322Abstract Sample barcoding is essential in mass cytometry analysis, since it can eliminate potential procedural variations, enhance throughput, and allow simultaneous sample processing and acquisition. Sample pooling after prior surface staining termed live-cell barcoding is more desirable than intracellular barcoding, where samples are pooled after fixation and permeabilization, since it does not depend on fixation-sensitive antigenic epitopes. In live-cell barcoding, the general approach uses two tags per sample out of a pool of antibodies paired with five palladium (Pd) isotopes in order to preserve appreciable signal-to-noise ratios and achieve higher yields after sample deconvolution. The number of samples that can be pooled in an experiment using live-cell barcoding is limited, due to weak signal intensities associated with Pd isotopes and the relatively low number of available tags. Here, we describe a novel barcoding technique utilizing 10 different tags, seven cadmium (Cd) tags and three Pd tags, with superior signal intensities that do not impinge on lanthanide detection, which enables enhanced pooling of samples with multiple experimental conditions and markedly enhances sample throughput.Muharrem MuftuogluLi LiShaoheng LiangDuncan MakAngelique J. LinJunxiang FangJared K. BurksKen ChenMichael AndreeffNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muharrem Muftuoglu Li Li Shaoheng Liang Duncan Mak Angelique J. Lin Junxiang Fang Jared K. Burks Ken Chen Michael Andreeff Extended live-cell barcoding approach for multiplexed mass cytometry
description	Abstract Sample barcoding is essential in mass cytometry analysis, since it can eliminate potential procedural variations, enhance throughput, and allow simultaneous sample processing and acquisition. Sample pooling after prior surface staining termed live-cell barcoding is more desirable than intracellular barcoding, where samples are pooled after fixation and permeabilization, since it does not depend on fixation-sensitive antigenic epitopes. In live-cell barcoding, the general approach uses two tags per sample out of a pool of antibodies paired with five palladium (Pd) isotopes in order to preserve appreciable signal-to-noise ratios and achieve higher yields after sample deconvolution. The number of samples that can be pooled in an experiment using live-cell barcoding is limited, due to weak signal intensities associated with Pd isotopes and the relatively low number of available tags. Here, we describe a novel barcoding technique utilizing 10 different tags, seven cadmium (Cd) tags and three Pd tags, with superior signal intensities that do not impinge on lanthanide detection, which enables enhanced pooling of samples with multiple experimental conditions and markedly enhances sample throughput.
format	article
author	Muharrem Muftuoglu Li Li Shaoheng Liang Duncan Mak Angelique J. Lin Junxiang Fang Jared K. Burks Ken Chen Michael Andreeff
author_facet	Muharrem Muftuoglu Li Li Shaoheng Liang Duncan Mak Angelique J. Lin Junxiang Fang Jared K. Burks Ken Chen Michael Andreeff
author_sort	Muharrem Muftuoglu
title	Extended live-cell barcoding approach for multiplexed mass cytometry
title_short	Extended live-cell barcoding approach for multiplexed mass cytometry
title_full	Extended live-cell barcoding approach for multiplexed mass cytometry
title_fullStr	Extended live-cell barcoding approach for multiplexed mass cytometry
title_full_unstemmed	Extended live-cell barcoding approach for multiplexed mass cytometry
title_sort	extended live-cell barcoding approach for multiplexed mass cytometry
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/95adbb71ff4b407bbd8d5e2b9fd48465
work_keys_str_mv	AT muharremmuftuoglu extendedlivecellbarcodingapproachformultiplexedmasscytometry AT lili extendedlivecellbarcodingapproachformultiplexedmasscytometry AT shaohengliang extendedlivecellbarcodingapproachformultiplexedmasscytometry AT duncanmak extendedlivecellbarcodingapproachformultiplexedmasscytometry AT angeliquejlin extendedlivecellbarcodingapproachformultiplexedmasscytometry AT junxiangfang extendedlivecellbarcodingapproachformultiplexedmasscytometry AT jaredkburks extendedlivecellbarcodingapproachformultiplexedmasscytometry AT kenchen extendedlivecellbarcodingapproachformultiplexedmasscytometry AT michaelandreeff extendedlivecellbarcodingapproachformultiplexedmasscytometry
_version_	1718380744949104640

Extended live-cell barcoding approach for multiplexed mass cytometry

Ejemplares similares