Development of RNAi method for screening candidate genes to control emerald ash borer, Agrilus planipennis

Abstract The ingestion of double-strand RNAs (dsRNA) targeting essential genes in an insect could cause mortality. Based on this principle, a new generation of insect control methods using RNA interference (RNAi) are being developed. In this work, we developed a bioassay for oral delivery of dsRNA t...

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Autores principales:	Thais B. Rodrigues, Lynne K. Rieske, Jian J. Duan, Kanakachari Mogilicherla, Subba R. Palli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95b00af95d7b4bf3b5282ecaced2c41b
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spelling	oai:doaj.org-article:95b00af95d7b4bf3b5282ecaced2c41b2021-12-02T11:52:25ZDevelopment of RNAi method for screening candidate genes to control emerald ash borer, Agrilus planipennis10.1038/s41598-017-07605-x2045-2322https://doaj.org/article/95b00af95d7b4bf3b5282ecaced2c41b2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07605-xhttps://doaj.org/toc/2045-2322Abstract The ingestion of double-strand RNAs (dsRNA) targeting essential genes in an insect could cause mortality. Based on this principle, a new generation of insect control methods using RNA interference (RNAi) are being developed. In this work, we developed a bioassay for oral delivery of dsRNA to an invasive forest and urban tree pest, the emerald ash borer (EAB, Agrilus planipennis). EAB feeds and develops beneath the bark, killing trees rapidly. This behavior, coupled with the lack of a reliable artificial diet for rearing larvae and adults, make them difficult to study. We found that dsRNA is transported and processed to siRNAs by EAB larvae within 72 h after ingestion. Also, feeding neonate larvae with IAP (inhibitor of apoptosis) or COP (COPI coatomer, β subunit) dsRNA silenced their target genes and caused mortality. Both an increase in the concentration of dsRNA fed and sequential feeding of two different dsRNAs increased mortality. Here we provide evidence for successful RNAi in EAB, and demonstrate the development of a rapid and effective bioassay for oral delivery of dsRNA to screen additional genes.Thais B. RodriguesLynne K. RieskeJian J. DuanKanakachari MogilicherlaSubba R. PalliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thais B. Rodrigues Lynne K. Rieske Jian J. Duan Kanakachari Mogilicherla Subba R. Palli Development of RNAi method for screening candidate genes to control emerald ash borer, Agrilus planipennis
description	Abstract The ingestion of double-strand RNAs (dsRNA) targeting essential genes in an insect could cause mortality. Based on this principle, a new generation of insect control methods using RNA interference (RNAi) are being developed. In this work, we developed a bioassay for oral delivery of dsRNA to an invasive forest and urban tree pest, the emerald ash borer (EAB, Agrilus planipennis). EAB feeds and develops beneath the bark, killing trees rapidly. This behavior, coupled with the lack of a reliable artificial diet for rearing larvae and adults, make them difficult to study. We found that dsRNA is transported and processed to siRNAs by EAB larvae within 72 h after ingestion. Also, feeding neonate larvae with IAP (inhibitor of apoptosis) or COP (COPI coatomer, β subunit) dsRNA silenced their target genes and caused mortality. Both an increase in the concentration of dsRNA fed and sequential feeding of two different dsRNAs increased mortality. Here we provide evidence for successful RNAi in EAB, and demonstrate the development of a rapid and effective bioassay for oral delivery of dsRNA to screen additional genes.
format	article
author	Thais B. Rodrigues Lynne K. Rieske Jian J. Duan Kanakachari Mogilicherla Subba R. Palli
author_facet	Thais B. Rodrigues Lynne K. Rieske Jian J. Duan Kanakachari Mogilicherla Subba R. Palli
author_sort	Thais B. Rodrigues
title	Development of RNAi method for screening candidate genes to control emerald ash borer, Agrilus planipennis
title_short	Development of RNAi method for screening candidate genes to control emerald ash borer, Agrilus planipennis
title_full	Development of RNAi method for screening candidate genes to control emerald ash borer, Agrilus planipennis
title_fullStr	Development of RNAi method for screening candidate genes to control emerald ash borer, Agrilus planipennis
title_full_unstemmed	Development of RNAi method for screening candidate genes to control emerald ash borer, Agrilus planipennis
title_sort	development of rnai method for screening candidate genes to control emerald ash borer, agrilus planipennis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/95b00af95d7b4bf3b5282ecaced2c41b
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Development of RNAi method for screening candidate genes to control emerald ash borer, Agrilus planipennis

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