Validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk

BACKGROUND: The information on official testing methods, or regulatory methods in Colombia to test whey in milk is limited; this restriction of information goes against the possibility of mitigating the risk of food fraud. OBJECTIVES: The validation of an HPLC method to determine casein glycomacrop...

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Autores principales:	Orlando Bernal Chia, Fernando Murcia Rubiano, Olga Lucía Ávila Guzmán, Jenifer Yaneth Guzman Gualteros, Willinton Arturo Arias Casallas, Maria Victoria Morales
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2021
Materias:	Casein glycomacropeptide c-GMP High Pressure Liquid Cromatographic - HPLC Fraud food Whey milk Raw bovine milk Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
Acceso en línea:	https://doaj.org/article/95b46da0a50b4b508aa2859ad22e1e49
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spelling	oai:doaj.org-article:95b46da0a50b4b508aa2859ad22e1e492021-12-02T18:20:59ZValidation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk10.17533/udea.vitae.v28n3a3452560121-40042145-2660https://doaj.org/article/95b46da0a50b4b508aa2859ad22e1e492021-08-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/vitae/article/view/345256https://doaj.org/toc/0121-4004https://doaj.org/toc/2145-2660 BACKGROUND: The information on official testing methods, or regulatory methods in Colombia to test whey in milk is limited; this restriction of information goes against the possibility of mitigating the risk of food fraud. OBJECTIVES: The validation of an HPLC method to determine casein glycomacropeptide (c-GMP), a protein that countries such as Brazil, Spain, and Ecuador have used as an indicator of raw milk adulteration with whey, was carried out. METHODS: A 10mL sample of raw milk is precipitated with 24% TCA using ultrasound, a process followed by filtration. The collected fraction ensured the separation of c-GMP and then injected into the liquid chromatography. RESULTS: A 30 minutes analysis allowed the determination of c-GMP with a retention time of 12.9 ± 0.5 minutes. The performance characteristics method in the validation exercise were: recovery percentage 99.97%, linearity R2> 0.95; % RSD accuracy <5.3%. CONCLUSION, the method exhibits desirable attributes for the intended purpose. Orlando Bernal ChiaFernando Murcia RubianoOlga Lucía Ávila GuzmánJenifer Yaneth Guzman GualterosWillinton Arturo Arias CasallasMaria Victoria MoralesUniversidad de AntioquiaarticleCasein glycomacropeptide c-GMPHigh Pressure Liquid Cromatographic - HPLCFraud foodWhey milkRaw bovine milkFood processing and manufactureTP368-456Pharmaceutical industryHD9665-9675ENVitae, Vol 28, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Casein glycomacropeptide c-GMP High Pressure Liquid Cromatographic - HPLC Fraud food Whey milk Raw bovine milk Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675
spellingShingle	Casein glycomacropeptide c-GMP High Pressure Liquid Cromatographic - HPLC Fraud food Whey milk Raw bovine milk Food processing and manufacture TP368-456 Pharmaceutical industry HD9665-9675 Orlando Bernal Chia Fernando Murcia Rubiano Olga Lucía Ávila Guzmán Jenifer Yaneth Guzman Gualteros Willinton Arturo Arias Casallas Maria Victoria Morales Validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk
description	BACKGROUND: The information on official testing methods, or regulatory methods in Colombia to test whey in milk is limited; this restriction of information goes against the possibility of mitigating the risk of food fraud. OBJECTIVES: The validation of an HPLC method to determine casein glycomacropeptide (c-GMP), a protein that countries such as Brazil, Spain, and Ecuador have used as an indicator of raw milk adulteration with whey, was carried out. METHODS: A 10mL sample of raw milk is precipitated with 24% TCA using ultrasound, a process followed by filtration. The collected fraction ensured the separation of c-GMP and then injected into the liquid chromatography. RESULTS: A 30 minutes analysis allowed the determination of c-GMP with a retention time of 12.9 ± 0.5 minutes. The performance characteristics method in the validation exercise were: recovery percentage 99.97%, linearity R2> 0.95; % RSD accuracy <5.3%. CONCLUSION, the method exhibits desirable attributes for the intended purpose.
format	article
author	Orlando Bernal Chia Fernando Murcia Rubiano Olga Lucía Ávila Guzmán Jenifer Yaneth Guzman Gualteros Willinton Arturo Arias Casallas Maria Victoria Morales
author_facet	Orlando Bernal Chia Fernando Murcia Rubiano Olga Lucía Ávila Guzmán Jenifer Yaneth Guzman Gualteros Willinton Arturo Arias Casallas Maria Victoria Morales
author_sort	Orlando Bernal Chia
title	Validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk
title_short	Validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk
title_full	Validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk
title_fullStr	Validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk
title_full_unstemmed	Validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk
title_sort	validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk
publisher	Universidad de Antioquia
publishDate	2021
url	https://doaj.org/article/95b46da0a50b4b508aa2859ad22e1e49
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Validation of a method employing liquid chromatography to identify and quantify glycomacropeptide of casein in raw milk

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