Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite

Abstract Chemical decontamination during processing is used in many countries to mitigate the Campylobacter load on chicken meat. Chlorine is a commonly used sanitizer in poultry processing to limit foodborne bacterial pathogens but its efficacy is limited by high bacterial loads and organic materia...

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Autores principales:	Gayani Weerasooriya, Andrea R. McWhorter, Samiullah Khan, Kapil K. Chousalkar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
Acceso en línea:	https://doaj.org/article/95b4cd6b1cc7444985e7a6e6f88d8a75
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spelling	oai:doaj.org-article:95b4cd6b1cc7444985e7a6e6f88d8a752021-12-02T18:49:17ZTranscriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite10.1038/s41538-021-00103-52396-8370https://doaj.org/article/95b4cd6b1cc7444985e7a6e6f88d8a752021-08-01T00:00:00Zhttps://doi.org/10.1038/s41538-021-00103-5https://doaj.org/toc/2396-8370Abstract Chemical decontamination during processing is used in many countries to mitigate the Campylobacter load on chicken meat. Chlorine is a commonly used sanitizer in poultry processing to limit foodborne bacterial pathogens but its efficacy is limited by high bacterial loads and organic material. Acidified sodium chlorite (ASC) is a potential alternative for poultry meat sanitization but little is known about its effects on the cellular response of Campylobacter. In this study, the sensitivity of C. jejuni isolates to ASC was established. RNAseq was performed to characterize the transcriptomic response of C. jejuni following exposure to either chlorine or ASC. Following chlorine exposure, C. jejuni induced an adaptive stress response mechanism. In contrast, exposure to ASC induced higher oxidative damage and cellular death by inhibiting all vital metabolic pathways and upregulating the genes involved in DNA damage and repair. The transcriptional changes in C. jejuni in response to ASC exposure suggest its potential as an effective sanitizer for use in the chicken meat industry.Gayani WeerasooriyaAndrea R. McWhorterSamiullah KhanKapil K. ChousalkarNature PortfolioarticleNutrition. Foods and food supplyTX341-641Food processing and manufactureTP368-456ENnpj Science of Food, Vol 5, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
spellingShingle	Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456 Gayani Weerasooriya Andrea R. McWhorter Samiullah Khan Kapil K. Chousalkar Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite
description	Abstract Chemical decontamination during processing is used in many countries to mitigate the Campylobacter load on chicken meat. Chlorine is a commonly used sanitizer in poultry processing to limit foodborne bacterial pathogens but its efficacy is limited by high bacterial loads and organic material. Acidified sodium chlorite (ASC) is a potential alternative for poultry meat sanitization but little is known about its effects on the cellular response of Campylobacter. In this study, the sensitivity of C. jejuni isolates to ASC was established. RNAseq was performed to characterize the transcriptomic response of C. jejuni following exposure to either chlorine or ASC. Following chlorine exposure, C. jejuni induced an adaptive stress response mechanism. In contrast, exposure to ASC induced higher oxidative damage and cellular death by inhibiting all vital metabolic pathways and upregulating the genes involved in DNA damage and repair. The transcriptional changes in C. jejuni in response to ASC exposure suggest its potential as an effective sanitizer for use in the chicken meat industry.
format	article
author	Gayani Weerasooriya Andrea R. McWhorter Samiullah Khan Kapil K. Chousalkar
author_facet	Gayani Weerasooriya Andrea R. McWhorter Samiullah Khan Kapil K. Chousalkar
author_sort	Gayani Weerasooriya
title	Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite
title_short	Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite
title_full	Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite
title_fullStr	Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite
title_full_unstemmed	Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite
title_sort	transcriptomic response of campylobacter jejuni following exposure to acidified sodium chlorite
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/95b4cd6b1cc7444985e7a6e6f88d8a75
work_keys_str_mv	AT gayaniweerasooriya transcriptomicresponseofcampylobacterjejunifollowingexposuretoacidifiedsodiumchlorite AT andrearmcwhorter transcriptomicresponseofcampylobacterjejunifollowingexposuretoacidifiedsodiumchlorite AT samiullahkhan transcriptomicresponseofcampylobacterjejunifollowingexposuretoacidifiedsodiumchlorite AT kapilkchousalkar transcriptomicresponseofcampylobacterjejunifollowingexposuretoacidifiedsodiumchlorite
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Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite

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