Alterations of mass density and 3D osteocyte lacunar properties in bisphosphonate-related osteonecrotic human jaw bone, a synchrotron µCT study.
Osteonecrosis of the jaw, in association with bisphosphonates (BRONJ) used for treating osteoporosis or cancer, is a severe and most often irreversible side effect whose underlying pathophysiological mechanisms remain largely unknown. Osteocytes are involved in bone remodeling and mineralization whe...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2014
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/95b5d9f4dd4b46e7bad1dc26f90fd63a |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:95b5d9f4dd4b46e7bad1dc26f90fd63a |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:95b5d9f4dd4b46e7bad1dc26f90fd63a2021-11-18T08:31:37ZAlterations of mass density and 3D osteocyte lacunar properties in bisphosphonate-related osteonecrotic human jaw bone, a synchrotron µCT study.1932-620310.1371/journal.pone.0088481https://doaj.org/article/95b5d9f4dd4b46e7bad1dc26f90fd63a2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24586331/?tool=EBIhttps://doaj.org/toc/1932-6203Osteonecrosis of the jaw, in association with bisphosphonates (BRONJ) used for treating osteoporosis or cancer, is a severe and most often irreversible side effect whose underlying pathophysiological mechanisms remain largely unknown. Osteocytes are involved in bone remodeling and mineralization where they orchestrate the delicate equilibrium between osteoclast and osteoblast activity and through the active process called osteocytic osteolysis. Here, we hypothesized that (i) changes of the mineralized tissue matrix play a substantial role in the pathogenesis of BRONJ, and (ii) the osteocyte lacunar morphology is altered in BRONJ. Synchrotron µCT with phase contrast is an appropriate tool for assessing both the 3D morphology of the osteocyte lacunae and the bone matrix mass density. Here, we used this technique to investigate the mass density distribution and 3D osteocyte lacunar properties at the sub-micrometer scale in human bone samples from the jaw, femur and tibia. First, we compared healthy human jaw bone to human tibia and femur in order to assess the specific differences and address potential explanations of why the jaw bone is exclusively targeted by the necrosis as a side effect of BP treatment. Second, we investigated the differences between BRONJ and control jaw bone samples to detect potential differences which could aid an improved understanding of the course of BRONJ. We found that the apparent mass density of jaw bone was significantly smaller compared to that of tibia, consistent with a higher bone turnover in the jaw bone. The variance of the lacunar volume distribution was significantly different depending on the anatomical site. The comparison between BRONJ and control jaw specimens revealed no significant increase in mineralization after BP. We found a significant decrease in osteocyte-lacunar density in the BRONJ group compared to the control jaw. Interestingly, the osteocyte-lacunar volume distribution was not altered after BP treatment.Bernhard HesseMax LangerPeter VargaAlexandra PacureanuPei DongSusanne SchrofNils MännickeHeikki SuhonenCecile OlivierPeter MaurerGalateia J KazakiaKay RaumFrancoise PeyrinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88481 (2014) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Bernhard Hesse Max Langer Peter Varga Alexandra Pacureanu Pei Dong Susanne Schrof Nils Männicke Heikki Suhonen Cecile Olivier Peter Maurer Galateia J Kazakia Kay Raum Francoise Peyrin Alterations of mass density and 3D osteocyte lacunar properties in bisphosphonate-related osteonecrotic human jaw bone, a synchrotron µCT study. |
| description |
Osteonecrosis of the jaw, in association with bisphosphonates (BRONJ) used for treating osteoporosis or cancer, is a severe and most often irreversible side effect whose underlying pathophysiological mechanisms remain largely unknown. Osteocytes are involved in bone remodeling and mineralization where they orchestrate the delicate equilibrium between osteoclast and osteoblast activity and through the active process called osteocytic osteolysis. Here, we hypothesized that (i) changes of the mineralized tissue matrix play a substantial role in the pathogenesis of BRONJ, and (ii) the osteocyte lacunar morphology is altered in BRONJ. Synchrotron µCT with phase contrast is an appropriate tool for assessing both the 3D morphology of the osteocyte lacunae and the bone matrix mass density. Here, we used this technique to investigate the mass density distribution and 3D osteocyte lacunar properties at the sub-micrometer scale in human bone samples from the jaw, femur and tibia. First, we compared healthy human jaw bone to human tibia and femur in order to assess the specific differences and address potential explanations of why the jaw bone is exclusively targeted by the necrosis as a side effect of BP treatment. Second, we investigated the differences between BRONJ and control jaw bone samples to detect potential differences which could aid an improved understanding of the course of BRONJ. We found that the apparent mass density of jaw bone was significantly smaller compared to that of tibia, consistent with a higher bone turnover in the jaw bone. The variance of the lacunar volume distribution was significantly different depending on the anatomical site. The comparison between BRONJ and control jaw specimens revealed no significant increase in mineralization after BP. We found a significant decrease in osteocyte-lacunar density in the BRONJ group compared to the control jaw. Interestingly, the osteocyte-lacunar volume distribution was not altered after BP treatment. |
| format |
article |
| author |
Bernhard Hesse Max Langer Peter Varga Alexandra Pacureanu Pei Dong Susanne Schrof Nils Männicke Heikki Suhonen Cecile Olivier Peter Maurer Galateia J Kazakia Kay Raum Francoise Peyrin |
| author_facet |
Bernhard Hesse Max Langer Peter Varga Alexandra Pacureanu Pei Dong Susanne Schrof Nils Männicke Heikki Suhonen Cecile Olivier Peter Maurer Galateia J Kazakia Kay Raum Francoise Peyrin |
| author_sort |
Bernhard Hesse |
| title |
Alterations of mass density and 3D osteocyte lacunar properties in bisphosphonate-related osteonecrotic human jaw bone, a synchrotron µCT study. |
| title_short |
Alterations of mass density and 3D osteocyte lacunar properties in bisphosphonate-related osteonecrotic human jaw bone, a synchrotron µCT study. |
| title_full |
Alterations of mass density and 3D osteocyte lacunar properties in bisphosphonate-related osteonecrotic human jaw bone, a synchrotron µCT study. |
| title_fullStr |
Alterations of mass density and 3D osteocyte lacunar properties in bisphosphonate-related osteonecrotic human jaw bone, a synchrotron µCT study. |
| title_full_unstemmed |
Alterations of mass density and 3D osteocyte lacunar properties in bisphosphonate-related osteonecrotic human jaw bone, a synchrotron µCT study. |
| title_sort |
alterations of mass density and 3d osteocyte lacunar properties in bisphosphonate-related osteonecrotic human jaw bone, a synchrotron µct study. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/95b5d9f4dd4b46e7bad1dc26f90fd63a |
| work_keys_str_mv |
AT bernhardhesse alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT maxlanger alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT petervarga alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT alexandrapacureanu alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT peidong alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT susanneschrof alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT nilsmannicke alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT heikkisuhonen alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT cecileolivier alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT petermaurer alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT galateiajkazakia alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT kayraum alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy AT francoisepeyrin alterationsofmassdensityand3dosteocytelacunarpropertiesinbisphosphonaterelatedosteonecrotichumanjawboneasynchrotronμctstudy |
| _version_ |
1718421697479049216 |