Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity

The hypothalamus plays a central role in the integrated regulation of feeding and energy homeostasis. The hypothalamic arcuate nucleus (ARC) contains a population of neurons that express orexigenic and anorexigenic factors and is thought to control feeding behavior via several neuronal circuits. In...

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Autores principales:	Chang Yeon Kim, Jang Ho Ahn, Do Hyun Han, Cherl NamKoong, Hyung Jin Choi
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/95b9b77a0a414d0daac1d089f63f5532
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spelling	oai:doaj.org-article:95b9b77a0a414d0daac1d089f63f55322021-11-29T00:56:14ZProteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity2314-614110.1155/2021/3501770https://doaj.org/article/95b9b77a0a414d0daac1d089f63f55322021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/3501770https://doaj.org/toc/2314-6141The hypothalamus plays a central role in the integrated regulation of feeding and energy homeostasis. The hypothalamic arcuate nucleus (ARC) contains a population of neurons that express orexigenic and anorexigenic factors and is thought to control feeding behavior via several neuronal circuits. In this study, a comparative proteomic analysis of low-fat control diet- (LFD-) and high-fat diet- (HFD-) induced hypothalamic ARC was performed to identify differentially expressed proteins (DEPs) related to changes in body weight. In the ARC in the hypothalamus, 6621 proteins (FDR<0.01) were detected, and 178 proteins were categorized as DEPs (89 upregulated and 89 downregulated in the HFD group). Among the Gene Ontology molecular function terms associated with the DEPs, protein binding was the most significant. Fibroblast growth factor receptor substrate 2 (Frs2) and SHC adaptor protein 3 (Shc3) were related to protein binding and involved in the neurotrophin signaling pathway according to Kyoto Encyclopedia of Genes and Genomes analysis. Furthermore, high-precision quantitative proteomic analysis revealed that the protein profile of the ARC in mice with HFD-induced obesity differed from that in LFD mice, thereby offering insight into the molecular basis of feeding regulation and suggesting Frs2 and Shc3 as novel treatment targets for central anorexigenic signal induction.Chang Yeon KimJang Ho AhnDo Hyun HanCherl NamKoongHyung Jin ChoiHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Chang Yeon Kim Jang Ho Ahn Do Hyun Han Cherl NamKoong Hyung Jin Choi Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity
description	The hypothalamus plays a central role in the integrated regulation of feeding and energy homeostasis. The hypothalamic arcuate nucleus (ARC) contains a population of neurons that express orexigenic and anorexigenic factors and is thought to control feeding behavior via several neuronal circuits. In this study, a comparative proteomic analysis of low-fat control diet- (LFD-) and high-fat diet- (HFD-) induced hypothalamic ARC was performed to identify differentially expressed proteins (DEPs) related to changes in body weight. In the ARC in the hypothalamus, 6621 proteins (FDR<0.01) were detected, and 178 proteins were categorized as DEPs (89 upregulated and 89 downregulated in the HFD group). Among the Gene Ontology molecular function terms associated with the DEPs, protein binding was the most significant. Fibroblast growth factor receptor substrate 2 (Frs2) and SHC adaptor protein 3 (Shc3) were related to protein binding and involved in the neurotrophin signaling pathway according to Kyoto Encyclopedia of Genes and Genomes analysis. Furthermore, high-precision quantitative proteomic analysis revealed that the protein profile of the ARC in mice with HFD-induced obesity differed from that in LFD mice, thereby offering insight into the molecular basis of feeding regulation and suggesting Frs2 and Shc3 as novel treatment targets for central anorexigenic signal induction.
format	article
author	Chang Yeon Kim Jang Ho Ahn Do Hyun Han Cherl NamKoong Hyung Jin Choi
author_facet	Chang Yeon Kim Jang Ho Ahn Do Hyun Han Cherl NamKoong Hyung Jin Choi
author_sort	Chang Yeon Kim
title	Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity
title_short	Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity
title_full	Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity
title_fullStr	Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity
title_full_unstemmed	Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity
title_sort	proteome analysis of the hypothalamic arcuate nucleus in chronic high-fat diet-induced obesity
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/95b9b77a0a414d0daac1d089f63f5532
work_keys_str_mv	AT changyeonkim proteomeanalysisofthehypothalamicarcuatenucleusinchronichighfatdietinducedobesity AT janghoahn proteomeanalysisofthehypothalamicarcuatenucleusinchronichighfatdietinducedobesity AT dohyunhan proteomeanalysisofthehypothalamicarcuatenucleusinchronichighfatdietinducedobesity AT cherlnamkoong proteomeanalysisofthehypothalamicarcuatenucleusinchronichighfatdietinducedobesity AT hyungjinchoi proteomeanalysisofthehypothalamicarcuatenucleusinchronichighfatdietinducedobesity
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Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity

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