Comparison of laboratory methods for quantification of fiber in feedstuffs
Background: Peter Van Soest proposed the conventional method of fiber analysis using neutral detergent fiber (NDF) and acid detergent fiber (ADF). The main advantage of this method is the precision of results; however, reagents are costly and laboratory work requires long runtime and labor. Objectiv...
Guardado en:
Autores principales: | , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Universidad de Antioquia
2017
|
Materias: | |
Acceso en línea: | https://doaj.org/article/95c10ee7afe94f20b3296e5079463dd3 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:95c10ee7afe94f20b3296e5079463dd3 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:95c10ee7afe94f20b3296e5079463dd32021-11-26T19:13:36ZComparison of laboratory methods for quantification of fiber in feedstuffs2256-295810.17533/udea.rccp.v30n1a03https://doaj.org/article/95c10ee7afe94f20b3296e5079463dd32017-01-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/325752https://doaj.org/toc/2256-2958Background: Peter Van Soest proposed the conventional method of fiber analysis using neutral detergent fiber (NDF) and acid detergent fiber (ADF). The main advantage of this method is the precision of results; however, reagents are costly and laboratory work requires long runtime and labor. Objective: to compare analytical data from different methodologies used to assess NDF and ADF contents. Methods: means obtained with the conventional method were compared through Dunnett’s test (α = 5%) with values from alternative methods using autoclave as the digester system. A completely randomized design in a 4 x 2 factorial arrangement was used. Results: NDF content through alternative methods was assessed for Tifton 85 hay, babassu meal and sugarcane, whereas ADF was only determined for babassu meal. NDF and ADF did not differ (p ≥ 0.05) between non-sequential or sequential analysis for all the feedstuffs and methods studied, except for ADF in corn silage. Conclusion: alternative methodologies allow reducing operating costs and time but lack uniformity and accuracy for analyzing ADF in corn silage. Palavras chave:Maria do Socorro N. LourençoJuliana D. MessanaAna Paula de O. SaderRoberta C. CanesinEuclides B. MalheirosPablo S. CastagninoTelma T. BerchielliUniversidad de Antioquiaarticleacid detergent fiberbabassu mealneutral detergent fibersugarcanetifton 85 hayAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 30, Iss 1, Pp 21-29 (2017) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
acid detergent fiber babassu meal neutral detergent fiber sugarcane tifton 85 hay Animal culture SF1-1100 |
spellingShingle |
acid detergent fiber babassu meal neutral detergent fiber sugarcane tifton 85 hay Animal culture SF1-1100 Maria do Socorro N. Lourenço Juliana D. Messana Ana Paula de O. Sader Roberta C. Canesin Euclides B. Malheiros Pablo S. Castagnino Telma T. Berchielli Comparison of laboratory methods for quantification of fiber in feedstuffs |
description |
Background: Peter Van Soest proposed the conventional method of fiber analysis using neutral detergent fiber (NDF) and acid detergent fiber (ADF). The main advantage of this method is the precision of results; however, reagents are costly and laboratory work requires long runtime and labor. Objective: to compare analytical data from different methodologies used to assess NDF and ADF contents. Methods: means obtained with the conventional method were compared through Dunnett’s test (α = 5%) with values from alternative methods using autoclave as the digester system. A completely randomized design in a 4 x 2 factorial arrangement was used. Results: NDF content through alternative methods was assessed for Tifton 85 hay, babassu meal and sugarcane, whereas ADF was only determined for babassu meal. NDF and ADF did not differ (p ≥ 0.05) between non-sequential or sequential analysis for all the feedstuffs and methods studied, except for ADF in corn silage. Conclusion: alternative methodologies allow reducing operating costs and time but lack uniformity and accuracy for analyzing ADF in corn silage.
Palavras chave: |
format |
article |
author |
Maria do Socorro N. Lourenço Juliana D. Messana Ana Paula de O. Sader Roberta C. Canesin Euclides B. Malheiros Pablo S. Castagnino Telma T. Berchielli |
author_facet |
Maria do Socorro N. Lourenço Juliana D. Messana Ana Paula de O. Sader Roberta C. Canesin Euclides B. Malheiros Pablo S. Castagnino Telma T. Berchielli |
author_sort |
Maria do Socorro N. Lourenço |
title |
Comparison of laboratory methods for quantification of fiber in feedstuffs |
title_short |
Comparison of laboratory methods for quantification of fiber in feedstuffs |
title_full |
Comparison of laboratory methods for quantification of fiber in feedstuffs |
title_fullStr |
Comparison of laboratory methods for quantification of fiber in feedstuffs |
title_full_unstemmed |
Comparison of laboratory methods for quantification of fiber in feedstuffs |
title_sort |
comparison of laboratory methods for quantification of fiber in feedstuffs |
publisher |
Universidad de Antioquia |
publishDate |
2017 |
url |
https://doaj.org/article/95c10ee7afe94f20b3296e5079463dd3 |
work_keys_str_mv |
AT mariadosocorronlourenco comparisonoflaboratorymethodsforquantificationoffiberinfeedstuffs AT julianadmessana comparisonoflaboratorymethodsforquantificationoffiberinfeedstuffs AT anapauladeosader comparisonoflaboratorymethodsforquantificationoffiberinfeedstuffs AT robertaccanesin comparisonoflaboratorymethodsforquantificationoffiberinfeedstuffs AT euclidesbmalheiros comparisonoflaboratorymethodsforquantificationoffiberinfeedstuffs AT pabloscastagnino comparisonoflaboratorymethodsforquantificationoffiberinfeedstuffs AT telmatberchielli comparisonoflaboratorymethodsforquantificationoffiberinfeedstuffs |
_version_ |
1718409305998229504 |