Determination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production

The functions and profiles of lncRNAs during infectious bursal disease virus (IBDV) infection have not been determined, yet. The objectives of this study were to determine the antiviral action of loc107051710 lncRNA during IBDV infection by investigating the relationship between loc107051710 and IRF...

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Autores principales:	Xuewei Huang, Yigang Xu, Qingyu Lin, Weilong Guo, Dongfang Zhao, Chunmei Wang, Li Wang, Han Zhou, Yanping Jiang, Wen Cui, Xinyuan Qiao, Yijing Li, Guangpeng Ma, Lijie Tang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	infectious bursal disease virus lncrna loc107051710 irf8 type i interferon interferon-stimulated gene Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/95c5470371184756b7705dab8bd0a0f8
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spelling	oai:doaj.org-article:95c5470371184756b7705dab8bd0a0f82021-11-17T14:21:57ZDetermination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production2150-55942150-560810.1080/21505594.2019.1707957https://doaj.org/article/95c5470371184756b7705dab8bd0a0f82020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2019.1707957https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608The functions and profiles of lncRNAs during infectious bursal disease virus (IBDV) infection have not been determined, yet. The objectives of this study were to determine the antiviral action of loc107051710 lncRNA during IBDV infection by investigating the relationship between loc107051710 and IRF8, Type I IFN, STATs, and ISGs. DF-1 cells were either left untreated as non-infected controls (n = 1) or infected with IBDV (n = 3). RNA sequencing was applied for analysis of mRNAs and lncRNAs expression. Differentially expressed genes were verified by RT-qPCR. Then identification, of 230 significantly different expressed genes (182 mRNAs and 48 lncRNA) by pairwise comparison of the infected and control groups, was carried out. The functions of differentially expressed lncRNAs were investigated by selection of lncRNAs and mRNAs significantly enriched in the aforementioned biological processes and signaling pathways for construction of lncRNA-mRNA co-expression networks. The techniques of gene ontology and Kyoto Encyclopedia of Genes and Genomes pathways were applied. It was suggested that these differentially expressed genes were involved in the interaction between the host and IBDV. Loc107051710 was found to have potential antiviral effects. RT-qPCR and western blot were applied and revealed that loc107051710 was required for induction of IRF8, type I IFN, STAT, and ISG expression, and its knockdown promoted IBDV replication. By fluorescence in situ hybridization, it was found that loc107051710 was translocated from the nucleus to the cytoplasm after infection with IBDV. Overall, loc107051710 promoted the production of IFN-α and IFN-β by regulating IRF8, thereby promoting the antiviral activity of ISGs.Xuewei HuangYigang XuQingyu LinWeilong GuoDongfang ZhaoChunmei WangLi WangHan ZhouYanping JiangWen CuiXinyuan QiaoYijing LiGuangpeng MaLijie TangTaylor & Francis Grouparticleinfectious bursal disease viruslncrnaloc107051710irf8type i interferoninterferon-stimulated geneInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 68-79 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	infectious bursal disease virus lncrna loc107051710 irf8 type i interferon interferon-stimulated gene Infectious and parasitic diseases RC109-216
spellingShingle	infectious bursal disease virus lncrna loc107051710 irf8 type i interferon interferon-stimulated gene Infectious and parasitic diseases RC109-216 Xuewei Huang Yigang Xu Qingyu Lin Weilong Guo Dongfang Zhao Chunmei Wang Li Wang Han Zhou Yanping Jiang Wen Cui Xinyuan Qiao Yijing Li Guangpeng Ma Lijie Tang Determination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production
description	The functions and profiles of lncRNAs during infectious bursal disease virus (IBDV) infection have not been determined, yet. The objectives of this study were to determine the antiviral action of loc107051710 lncRNA during IBDV infection by investigating the relationship between loc107051710 and IRF8, Type I IFN, STATs, and ISGs. DF-1 cells were either left untreated as non-infected controls (n = 1) or infected with IBDV (n = 3). RNA sequencing was applied for analysis of mRNAs and lncRNAs expression. Differentially expressed genes were verified by RT-qPCR. Then identification, of 230 significantly different expressed genes (182 mRNAs and 48 lncRNA) by pairwise comparison of the infected and control groups, was carried out. The functions of differentially expressed lncRNAs were investigated by selection of lncRNAs and mRNAs significantly enriched in the aforementioned biological processes and signaling pathways for construction of lncRNA-mRNA co-expression networks. The techniques of gene ontology and Kyoto Encyclopedia of Genes and Genomes pathways were applied. It was suggested that these differentially expressed genes were involved in the interaction between the host and IBDV. Loc107051710 was found to have potential antiviral effects. RT-qPCR and western blot were applied and revealed that loc107051710 was required for induction of IRF8, type I IFN, STAT, and ISG expression, and its knockdown promoted IBDV replication. By fluorescence in situ hybridization, it was found that loc107051710 was translocated from the nucleus to the cytoplasm after infection with IBDV. Overall, loc107051710 promoted the production of IFN-α and IFN-β by regulating IRF8, thereby promoting the antiviral activity of ISGs.
format	article
author	Xuewei Huang Yigang Xu Qingyu Lin Weilong Guo Dongfang Zhao Chunmei Wang Li Wang Han Zhou Yanping Jiang Wen Cui Xinyuan Qiao Yijing Li Guangpeng Ma Lijie Tang
author_facet	Xuewei Huang Yigang Xu Qingyu Lin Weilong Guo Dongfang Zhao Chunmei Wang Li Wang Han Zhou Yanping Jiang Wen Cui Xinyuan Qiao Yijing Li Guangpeng Ma Lijie Tang
author_sort	Xuewei Huang
title	Determination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production
title_short	Determination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production
title_full	Determination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production
title_fullStr	Determination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production
title_full_unstemmed	Determination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production
title_sort	determination of antiviral action of long non-coding rna loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/95c5470371184756b7705dab8bd0a0f8
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Determination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production

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