Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)

Consumption of contaminated meat, milk, and water are among the major routes of human campylobacteriosis. This study aimed to determined the genetic diversity of C. coli and C. jejuni isolated from meat, milk, and water samples collected from different locations. From the 376 samples (meat = 248, co...

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Autores principales:	Aboi Igwaran, Anthony Ifeanyi Okoh
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	campylobacter dendrogram diversity cluster eric-pcr genotyping Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/95d336b903ed477d90480faa0983feda
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spelling	oai:doaj.org-article:95d336b903ed477d90480faa0983feda2021-11-04T15:00:43ZMolecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)2000-868610.1080/20008686.2020.1830701https://doaj.org/article/95d336b903ed477d90480faa0983feda2020-01-01T00:00:00Zhttp://dx.doi.org/10.1080/20008686.2020.1830701https://doaj.org/toc/2000-8686Consumption of contaminated meat, milk, and water are among the major routes of human campylobacteriosis. This study aimed to determined the genetic diversity of C. coli and C. jejuni isolated from meat, milk, and water samples collected from different locations. From the 376 samples (meat = 248, cow milk = 72, and water = 56) collected, a total of 1238 presumptive Campylobacter isolates were recovered and the presence of the genus Campylobacter were detected in 402 isolates, and from which, 85 and 67 isolates were identified asC. jejuni and C. coli respectively. Of which, 71 isolates identified as C. coli (n = 35) and C. jejuni (n = 36) were randomly selected from meat, milk, and water samples and were genotyped using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The digital images of the ERIC-PCR genotype were analyzed by GelJ v.2.0 software. The diversity and similarity of the isolates were assessed via an unweighted-pair group method using average linkages clustering algorithm. The results showed that the 36 C. jejuni strains separated into 29 ERIC-genotypes and 4 clusters while the 35 C. coli were delineated into 29 ERIC-genotypes and 6 clusters. The study revealed the genetic diversity among C. coli and C. jejuni strains recovered from different matrices characterized by Gelj.Aboi IgwaranAnthony Ifeanyi OkohTaylor & Francis Grouparticlecampylobacterdendrogramdiversityclustereric-pcrgenotypingInfectious and parasitic diseasesRC109-216ENInfection Ecology & Epidemiology, Vol 10, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	campylobacter dendrogram diversity cluster eric-pcr genotyping Infectious and parasitic diseases RC109-216
spellingShingle	campylobacter dendrogram diversity cluster eric-pcr genotyping Infectious and parasitic diseases RC109-216 Aboi Igwaran Anthony Ifeanyi Okoh Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)
description	Consumption of contaminated meat, milk, and water are among the major routes of human campylobacteriosis. This study aimed to determined the genetic diversity of C. coli and C. jejuni isolated from meat, milk, and water samples collected from different locations. From the 376 samples (meat = 248, cow milk = 72, and water = 56) collected, a total of 1238 presumptive Campylobacter isolates were recovered and the presence of the genus Campylobacter were detected in 402 isolates, and from which, 85 and 67 isolates were identified asC. jejuni and C. coli respectively. Of which, 71 isolates identified as C. coli (n = 35) and C. jejuni (n = 36) were randomly selected from meat, milk, and water samples and were genotyped using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The digital images of the ERIC-PCR genotype were analyzed by GelJ v.2.0 software. The diversity and similarity of the isolates were assessed via an unweighted-pair group method using average linkages clustering algorithm. The results showed that the 36 C. jejuni strains separated into 29 ERIC-genotypes and 4 clusters while the 35 C. coli were delineated into 29 ERIC-genotypes and 6 clusters. The study revealed the genetic diversity among C. coli and C. jejuni strains recovered from different matrices characterized by Gelj.
format	article
author	Aboi Igwaran Anthony Ifeanyi Okoh
author_facet	Aboi Igwaran Anthony Ifeanyi Okoh
author_sort	Aboi Igwaran
title	Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)
title_short	Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)
title_full	Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)
title_fullStr	Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)
title_full_unstemmed	Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)
title_sort	molecular determination of genetic diversity among campylobacter jejuni and campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus pcr (eric-pcr)
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/95d336b903ed477d90480faa0983feda
work_keys_str_mv	AT aboiigwaran moleculardeterminationofgeneticdiversityamongcampylobacterjejuniandcampylobactercoliisolatedfrommilkwaterandmeatsamplesusingenterobacterialrepetitiveintergenicconsensuspcrericpcr AT anthonyifeanyiokoh moleculardeterminationofgeneticdiversityamongcampylobacterjejuniandcampylobactercoliisolatedfrommilkwaterandmeatsamplesusingenterobacterialrepetitiveintergenicconsensuspcrericpcr
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Molecular determination of genetic diversity among Campylobacter jejuni and Campylobacter coli isolated from milk, water, and meat samples using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR)

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