A molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep.

The accurate diagnosis of parasitic nematode infections in livestock (including sheep and goats) is central to their effective control and the detection of the anthelmintic resistance. Traditionally, the faecal egg count reduction test (FECRT), combined with the technique of larval culture (LC), has...

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Autores principales:	Florian Roeber, John W A Larsen, Norman Anderson, Angus J D Campbell, Garry A Anderson, Robin B Gasser, Aaron R Jex
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95d6cdce012044db9c67d568d13f6723
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spelling	oai:doaj.org-article:95d6cdce012044db9c67d568d13f67232021-11-18T07:17:50ZA molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep.1932-620310.1371/journal.pone.0037327https://doaj.org/article/95d6cdce012044db9c67d568d13f67232012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22629381/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The accurate diagnosis of parasitic nematode infections in livestock (including sheep and goats) is central to their effective control and the detection of the anthelmintic resistance. Traditionally, the faecal egg count reduction test (FECRT), combined with the technique of larval culture (LC), has been used widely to assess drug-susceptibility/resistance in strongylid nematodes. However, this approach suffers from a lack of specificity, sensitivity and reliability, and is time-consuming and costly to conduct. Here, we critically assessed a specific PCR assay to support FECRT, in a well-controlled experiment on sheep with naturally acquired strongylid infections known to be resistant to benzimidazoles. We showed that the PCR results were in close agreement with those of total worm count (TWC), but not of LC. Importantly, albendazole resistance detected by PCR-coupled FECRT was unequivocally linked to Teladorsagia circumcincta and, to lesser extent, Trichostrongylus colubriformis, a result that was not achievable by LC. The key findings from this study demonstrate that our PCR-coupled FECRT approach has major merit for supporting anthelmintic resistance in nematode populations. The findings also show clearly that our PCR assay can be used as an alternative to LC, and is more time-efficient and less laborious, which has important practical implications for the effective management and control strongylid nematodes of sheep.Florian RoeberJohn W A LarsenNorman AndersonAngus J D CampbellGarry A AndersonRobin B GasserAaron R JexPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37327 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Florian Roeber John W A Larsen Norman Anderson Angus J D Campbell Garry A Anderson Robin B Gasser Aaron R Jex A molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep.
description	The accurate diagnosis of parasitic nematode infections in livestock (including sheep and goats) is central to their effective control and the detection of the anthelmintic resistance. Traditionally, the faecal egg count reduction test (FECRT), combined with the technique of larval culture (LC), has been used widely to assess drug-susceptibility/resistance in strongylid nematodes. However, this approach suffers from a lack of specificity, sensitivity and reliability, and is time-consuming and costly to conduct. Here, we critically assessed a specific PCR assay to support FECRT, in a well-controlled experiment on sheep with naturally acquired strongylid infections known to be resistant to benzimidazoles. We showed that the PCR results were in close agreement with those of total worm count (TWC), but not of LC. Importantly, albendazole resistance detected by PCR-coupled FECRT was unequivocally linked to Teladorsagia circumcincta and, to lesser extent, Trichostrongylus colubriformis, a result that was not achievable by LC. The key findings from this study demonstrate that our PCR-coupled FECRT approach has major merit for supporting anthelmintic resistance in nematode populations. The findings also show clearly that our PCR assay can be used as an alternative to LC, and is more time-efficient and less laborious, which has important practical implications for the effective management and control strongylid nematodes of sheep.
format	article
author	Florian Roeber John W A Larsen Norman Anderson Angus J D Campbell Garry A Anderson Robin B Gasser Aaron R Jex
author_facet	Florian Roeber John W A Larsen Norman Anderson Angus J D Campbell Garry A Anderson Robin B Gasser Aaron R Jex
author_sort	Florian Roeber
title	A molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep.
title_short	A molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep.
title_full	A molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep.
title_fullStr	A molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep.
title_full_unstemmed	A molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep.
title_sort	molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/95d6cdce012044db9c67d568d13f6723
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A molecular diagnostic tool to replace larval culture in conventional faecal egg count reduction testing in sheep.

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