Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma

Abstract Camel milk has been gaining immmense importance due to high nutritious value and medicinal properties. Peptides from milk proteins is gaining popularity in various therapeutics including human cancer. The study was aimed to investigate the anti-cancerous and anti-inflammatory properties of...

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Autores principales:	Chandraprabha Murali, Priti Mudgil, Chee-Yuen Gan, Hamadeh Tarazi, Raafat El-Awady, Youssef Abdalla, Amr Amin, Sajid Maqsood
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95e60ad1e223423db53a4c905f7ddb74
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spelling	oai:doaj.org-article:95e60ad1e223423db53a4c905f7ddb742021-12-02T13:26:28ZCamel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma10.1038/s41598-021-86391-z2045-2322https://doaj.org/article/95e60ad1e223423db53a4c905f7ddb742021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86391-zhttps://doaj.org/toc/2045-2322Abstract Camel milk has been gaining immmense importance due to high nutritious value and medicinal properties. Peptides from milk proteins is gaining popularity in various therapeutics including human cancer. The study was aimed to investigate the anti-cancerous and anti-inflammatory properties of camel whey protein hydrolysates (CWPHs). CWPHs were generated at three temperatures (30 ℃, 37 ℃, and 45 ℃), two hydrolysis timepoints (120 and 360 min) and with three different enzyme concentrations (0.5, 1 and 2 %). CWPHs demonstrated an increase in anti-inflammatory effect between 732.50 (P-6.1) and 3779.16 (P-2.1) µg Dicolfenac Sodium Equivalent (DSE)/mg protein. CWPHs (P-4.3 & 5.2) inhibited growth of human colon carcinoma cells (HCT116) with an IC50 value of 231 and 221 μg/ml, respectively. P-4.3 induced G2/M cell cycle arrest and modulated the expression of Cdk1, p-Cdk1, Cyclin B1, p-histone H3, p21 and p53. Docking of two peptides (AHLEQVLLR and ALPNIDPPTVER) from CWPHs (P-4.3) identified Polo like kinase 1 as a potential target, which strongly supports our in vitro data and provides an encouraging insight into developing a novel peptide-based anticancer formulation. These results suggest that the active component, CWPHs (P-4.3), can be further studied and modeled to form a small molecule anti-cancerous therapy.Chandraprabha MuraliPriti MudgilChee-Yuen GanHamadeh TaraziRaafat El-AwadyYoussef AbdallaAmr AminSajid MaqsoodNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chandraprabha Murali Priti Mudgil Chee-Yuen Gan Hamadeh Tarazi Raafat El-Awady Youssef Abdalla Amr Amin Sajid Maqsood Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma
description	Abstract Camel milk has been gaining immmense importance due to high nutritious value and medicinal properties. Peptides from milk proteins is gaining popularity in various therapeutics including human cancer. The study was aimed to investigate the anti-cancerous and anti-inflammatory properties of camel whey protein hydrolysates (CWPHs). CWPHs were generated at three temperatures (30 ℃, 37 ℃, and 45 ℃), two hydrolysis timepoints (120 and 360 min) and with three different enzyme concentrations (0.5, 1 and 2 %). CWPHs demonstrated an increase in anti-inflammatory effect between 732.50 (P-6.1) and 3779.16 (P-2.1) µg Dicolfenac Sodium Equivalent (DSE)/mg protein. CWPHs (P-4.3 & 5.2) inhibited growth of human colon carcinoma cells (HCT116) with an IC50 value of 231 and 221 μg/ml, respectively. P-4.3 induced G2/M cell cycle arrest and modulated the expression of Cdk1, p-Cdk1, Cyclin B1, p-histone H3, p21 and p53. Docking of two peptides (AHLEQVLLR and ALPNIDPPTVER) from CWPHs (P-4.3) identified Polo like kinase 1 as a potential target, which strongly supports our in vitro data and provides an encouraging insight into developing a novel peptide-based anticancer formulation. These results suggest that the active component, CWPHs (P-4.3), can be further studied and modeled to form a small molecule anti-cancerous therapy.
format	article
author	Chandraprabha Murali Priti Mudgil Chee-Yuen Gan Hamadeh Tarazi Raafat El-Awady Youssef Abdalla Amr Amin Sajid Maqsood
author_facet	Chandraprabha Murali Priti Mudgil Chee-Yuen Gan Hamadeh Tarazi Raafat El-Awady Youssef Abdalla Amr Amin Sajid Maqsood
author_sort	Chandraprabha Murali
title	Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma
title_short	Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma
title_full	Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma
title_fullStr	Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma
title_full_unstemmed	Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma
title_sort	camel whey protein hydrolysates induced g2/m cellcycle arrest in human colorectal carcinoma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/95e60ad1e223423db53a4c905f7ddb74
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Camel whey protein hydrolysates induced G2/M cellcycle arrest in human colorectal carcinoma

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