Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.

RNA silencing, mediated by small RNAs including microRNAs (miRNAs) and small interfering RNAs (siRNAs), is a potent antiviral or antibacterial mechanism, besides regulating normal cellular gene expression critical for development and physiology. To gain insights into host small RNA metabolism under...

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Autores principales:	Peng Du, Jianguo Wu, Jiayao Zhang, Shuqi Zhao, Hong Zheng, Ge Gao, Liping Wei, Yi Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/95ea7f832f664360b7d18a683b55e0e4
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spelling	oai:doaj.org-article:95ea7f832f664360b7d18a683b55e0e42021-11-18T06:03:06ZViral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.1553-73661553-737410.1371/journal.ppat.1002176https://doaj.org/article/95ea7f832f664360b7d18a683b55e0e42011-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21901091/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374RNA silencing, mediated by small RNAs including microRNAs (miRNAs) and small interfering RNAs (siRNAs), is a potent antiviral or antibacterial mechanism, besides regulating normal cellular gene expression critical for development and physiology. To gain insights into host small RNA metabolism under infections by different viruses, we used Solexa/Illumina deep sequencing to characterize the small RNA profiles of rice plants infected by two distinct viruses, Rice dwarf virus (RDV, dsRNA virus) and Rice stripe virus (RSV, a negative sense and ambisense RNA virus), respectively, as compared with those from non-infected plants. Our analyses showed that RSV infection enhanced the accumulation of some rice miRNA*s, but not their corresponding miRNAs, as well as accumulation of phased siRNAs from a particular precursor. Furthermore, RSV infection also induced the expression of novel miRNAs in a phased pattern from several conserved miRNA precursors. In comparison, no such changes in host small RNA expression was observed in RDV-infected rice plants. Significantly RSV infection elevated the expression levels of selective OsDCLs and OsAGOs, whereas RDV infection only affected the expression of certain OsRDRs. Our results provide a comparative analysis, via deep sequencing, of changes in the small RNA profiles and in the genes of RNA silencing machinery induced by different viruses in a natural and economically important crop host plant. They uncover new mechanisms and complexity of virus-host interactions that may have important implications for further studies on the evolution of cellular small RNA biogenesis that impact pathogen infection, pathogenesis, as well as organismal development.Peng DuJianguo WuJiayao ZhangShuqi ZhaoHong ZhengGe GaoLiping WeiYi LiPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 7, Iss 8, p e1002176 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Peng Du Jianguo Wu Jiayao Zhang Shuqi Zhao Hong Zheng Ge Gao Liping Wei Yi Li Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.
description	RNA silencing, mediated by small RNAs including microRNAs (miRNAs) and small interfering RNAs (siRNAs), is a potent antiviral or antibacterial mechanism, besides regulating normal cellular gene expression critical for development and physiology. To gain insights into host small RNA metabolism under infections by different viruses, we used Solexa/Illumina deep sequencing to characterize the small RNA profiles of rice plants infected by two distinct viruses, Rice dwarf virus (RDV, dsRNA virus) and Rice stripe virus (RSV, a negative sense and ambisense RNA virus), respectively, as compared with those from non-infected plants. Our analyses showed that RSV infection enhanced the accumulation of some rice miRNA*s, but not their corresponding miRNAs, as well as accumulation of phased siRNAs from a particular precursor. Furthermore, RSV infection also induced the expression of novel miRNAs in a phased pattern from several conserved miRNA precursors. In comparison, no such changes in host small RNA expression was observed in RDV-infected rice plants. Significantly RSV infection elevated the expression levels of selective OsDCLs and OsAGOs, whereas RDV infection only affected the expression of certain OsRDRs. Our results provide a comparative analysis, via deep sequencing, of changes in the small RNA profiles and in the genes of RNA silencing machinery induced by different viruses in a natural and economically important crop host plant. They uncover new mechanisms and complexity of virus-host interactions that may have important implications for further studies on the evolution of cellular small RNA biogenesis that impact pathogen infection, pathogenesis, as well as organismal development.
format	article
author	Peng Du Jianguo Wu Jiayao Zhang Shuqi Zhao Hong Zheng Ge Gao Liping Wei Yi Li
author_facet	Peng Du Jianguo Wu Jiayao Zhang Shuqi Zhao Hong Zheng Ge Gao Liping Wei Yi Li
author_sort	Peng Du
title	Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.
title_short	Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.
title_full	Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.
title_fullStr	Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.
title_full_unstemmed	Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.
title_sort	viral infection induces expression of novel phased micrornas from conserved cellular microrna precursors.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/95ea7f832f664360b7d18a683b55e0e4
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_version_	1718424682098589696

Viral infection induces expression of novel phased microRNAs from conserved cellular microRNA precursors.

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