Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak
(1) Background: We determined the relevance of intestinal dominance by <i>Serratia</i> spp. during a neonatal outbreak over 13 weeks. (2) Methods: Rectal swabs (n = 110) were obtained from 42 neonates. <i>Serratia</i> spp. was cultured from swabs obtained from 13 neonates (Gr...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/95ee7026732240fcadeba90f269395e8 |
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| Sumario: | (1) Background: We determined the relevance of intestinal dominance by <i>Serratia</i> spp. during a neonatal outbreak over 13 weeks. (2) Methods: Rectal swabs (n = 110) were obtained from 42 neonates. <i>Serratia</i> spp. was cultured from swabs obtained from 13 neonates (Group 1), while the other 29 neonates were culture-negative (Group 2). Total DNA was extracted from rectal swabs, and quantitative PCRs (qPCRs) using <i>Serratia</i>- and <i>16SrRNA</i>-gene-specific primers were performed. relative intestinal loads (RLs) were determined using ΔΔC<sub>t</sub>. Clonality was investigated by random amplified polymorphic DNA analysis and whole-genome sequencing. (3) Results: The outbreak was caused by <i>Serratia marcescens</i> during the first eight weeks and <i>Serratia ureilytica</i> during the remaining five weeks. <i>Serratia</i> spp. were detected by qPCR in all Group 1 neonates and eleven Group 2 neonates. RLs of <i>Serratia</i> spp. were higher in Group 1 as compared to Group 2 (6.31% vs. 0.09%, <i>p</i> < 0.05) and in the first swab compared to the last (26.9% vs. 4.37%, <i>p</i> < 0.05). Nine neonates had extraintestinal detection of <i>Serratia</i> spp.; eight of them were infected. RLs of the patients with extraintestinal spread were higher than the rest (2.79% vs. 0.29%, <i>p</i> < 0.05). (4) Conclusions: Intestinal dominance by <i>Serratia</i> spp. plays a role in outbreaks and extraintestinal spread. |
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