Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak

Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak

(1) Background: We determined the relevance of intestinal dominance by <i>Serratia</i> spp. during a neonatal outbreak over 13 weeks. (2) Methods: Rectal swabs (n = 110) were obtained from 42 neonates. <i>Serratia</i> spp. was cultured from swabs obtained from 13 neonates (Gr...

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Autores principales: Elias Dahdouh, Fernando Lázaro-Perona, Guillermo Ruiz-Carrascoso, Laura Sánchez García, Miguel Saenz de Pipaón, Jesús Mingorance
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
<i>Serratia marcescens</i>
<i>Serratia ureilytica</i>
neonatal ward
intestinal dominance
qPCR
Biology (General)
QH301-705.5
Acceso en línea:https://doaj.org/article/95ee7026732240fcadeba90f269395e8
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spelling oai:doaj.org-article:95ee7026732240fcadeba90f269395e82021-11-25T18:24:45ZIntestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak10.3390/microorganisms91122712076-2607https://doaj.org/article/95ee7026732240fcadeba90f269395e82021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2271https://doaj.org/toc/2076-2607(1) Background: We determined the relevance of intestinal dominance by <i>Serratia</i> spp. during a neonatal outbreak over 13 weeks. (2) Methods: Rectal swabs (n = 110) were obtained from 42 neonates. <i>Serratia</i> spp. was cultured from swabs obtained from 13 neonates (Group 1), while the other 29 neonates were culture-negative (Group 2). Total DNA was extracted from rectal swabs, and quantitative PCRs (qPCRs) using <i>Serratia</i>- and <i>16SrRNA</i>-gene-specific primers were performed. relative intestinal loads (RLs) were determined using ΔΔC<sub>t</sub>. Clonality was investigated by random amplified polymorphic DNA analysis and whole-genome sequencing. (3) Results: The outbreak was caused by <i>Serratia marcescens</i> during the first eight weeks and <i>Serratia ureilytica</i> during the remaining five weeks. <i>Serratia</i> spp. were detected by qPCR in all Group 1 neonates and eleven Group 2 neonates. RLs of <i>Serratia</i> spp. were higher in Group 1 as compared to Group 2 (6.31% vs. 0.09%, <i>p</i> < 0.05) and in the first swab compared to the last (26.9% vs. 4.37%, <i>p</i> < 0.05). Nine neonates had extraintestinal detection of <i>Serratia</i> spp.; eight of them were infected. RLs of the patients with extraintestinal spread were higher than the rest (2.79% vs. 0.29%, <i>p</i> < 0.05). (4) Conclusions: Intestinal dominance by <i>Serratia</i> spp. plays a role in outbreaks and extraintestinal spread.Elias DahdouhFernando Lázaro-PeronaGuillermo Ruiz-CarrascosoLaura Sánchez GarcíaMiguel Saenz de PipaónJesús MingoranceMDPI AGarticle<i>Serratia marcescens</i><i>Serratia ureilytica</i>neonatal wardintestinal dominanceqPCRBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2271, p 2271 (2021)
institution DOAJ
collection DOAJ
language EN
topic <i>Serratia marcescens</i>
<i>Serratia ureilytica</i>
neonatal ward
intestinal dominance
qPCR
Biology (General)
QH301-705.5
spellingShingle <i>Serratia marcescens</i>
<i>Serratia ureilytica</i>
neonatal ward
intestinal dominance
qPCR
Biology (General)
QH301-705.5
Elias Dahdouh
Fernando Lázaro-Perona
Guillermo Ruiz-Carrascoso
Laura Sánchez García
Miguel Saenz de Pipaón
Jesús Mingorance
Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak
description (1) Background: We determined the relevance of intestinal dominance by <i>Serratia</i> spp. during a neonatal outbreak over 13 weeks. (2) Methods: Rectal swabs (n = 110) were obtained from 42 neonates. <i>Serratia</i> spp. was cultured from swabs obtained from 13 neonates (Group 1), while the other 29 neonates were culture-negative (Group 2). Total DNA was extracted from rectal swabs, and quantitative PCRs (qPCRs) using <i>Serratia</i>- and <i>16SrRNA</i>-gene-specific primers were performed. relative intestinal loads (RLs) were determined using ΔΔC<sub>t</sub>. Clonality was investigated by random amplified polymorphic DNA analysis and whole-genome sequencing. (3) Results: The outbreak was caused by <i>Serratia marcescens</i> during the first eight weeks and <i>Serratia ureilytica</i> during the remaining five weeks. <i>Serratia</i> spp. were detected by qPCR in all Group 1 neonates and eleven Group 2 neonates. RLs of <i>Serratia</i> spp. were higher in Group 1 as compared to Group 2 (6.31% vs. 0.09%, <i>p</i> < 0.05) and in the first swab compared to the last (26.9% vs. 4.37%, <i>p</i> < 0.05). Nine neonates had extraintestinal detection of <i>Serratia</i> spp.; eight of them were infected. RLs of the patients with extraintestinal spread were higher than the rest (2.79% vs. 0.29%, <i>p</i> < 0.05). (4) Conclusions: Intestinal dominance by <i>Serratia</i> spp. plays a role in outbreaks and extraintestinal spread.
format article
author Elias Dahdouh
Fernando Lázaro-Perona
Guillermo Ruiz-Carrascoso
Laura Sánchez García
Miguel Saenz de Pipaón
Jesús Mingorance
author_facet Elias Dahdouh
Fernando Lázaro-Perona
Guillermo Ruiz-Carrascoso
Laura Sánchez García
Miguel Saenz de Pipaón
Jesús Mingorance
author_sort Elias Dahdouh
title Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak
title_short Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak
title_full Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak
title_fullStr Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak
title_full_unstemmed Intestinal Dominance by <i>Serratia marcescens</i> and <i>Serratia ureilytica</i> among Neonates in the Setting of an Outbreak
title_sort intestinal dominance by <i>serratia marcescens</i> and <i>serratia ureilytica</i> among neonates in the setting of an outbreak
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/95ee7026732240fcadeba90f269395e8
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