Myc and max genome-wide binding sites analysis links the Myc regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells.

Myc is a master transcription factor that has been demonstrated to be required for embryonic stem cell (ESC) pluripotency, self-renewal, and inhibition of differentiation. Although recent works have identified several Myc-targets in ESCs, the list of Myc binding sites is largely incomplete due to th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Anna Krepelova, Francesco Neri, Mara Maldotti, Stefania Rapelli, Salvatore Oliviero
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95ef5c7e72b54ffdab9f00496f497130
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:95ef5c7e72b54ffdab9f00496f497130
record_format	dspace
spelling	oai:doaj.org-article:95ef5c7e72b54ffdab9f00496f4971302021-11-18T08:31:35ZMyc and max genome-wide binding sites analysis links the Myc regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells.1932-620310.1371/journal.pone.0088933https://doaj.org/article/95ef5c7e72b54ffdab9f00496f4971302014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24586446/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Myc is a master transcription factor that has been demonstrated to be required for embryonic stem cell (ESC) pluripotency, self-renewal, and inhibition of differentiation. Although recent works have identified several Myc-targets in ESCs, the list of Myc binding sites is largely incomplete due to the low sensitivity and specificity of the antibodies available. To systematically identify Myc binding sites in mouse ESCs, we used a stringent streptavidin-based genome-wide chromatin immunoprecipitation (ChIP-Seq) approach with biotin-tagged Myc (Bio-Myc) as well as a ChIP-Seq of the Myc binding partner Max. This analysis identified 4325 Myc binding sites, of which 2885 were newly identified. The identified sites overlap with more than 85% of the Max binding sites and are enriched for H3K4me3-positive promoters and active enhancers. Remarkably, this analysis unveils that Myc/Max regulates chromatin modifiers and transcriptional regulators involved in stem cell self-renewal linking the Myc-centered network with the Polycomb and the Core networks. These results provide insights into the contribution of Myc and Max in maintaining stem cell self-renewal and keeping these cells in an undifferentiated state.Anna KrepelovaFrancesco NeriMara MaldottiStefania RapelliSalvatore OlivieroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88933 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anna Krepelova Francesco Neri Mara Maldotti Stefania Rapelli Salvatore Oliviero Myc and max genome-wide binding sites analysis links the Myc regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells.
description	Myc is a master transcription factor that has been demonstrated to be required for embryonic stem cell (ESC) pluripotency, self-renewal, and inhibition of differentiation. Although recent works have identified several Myc-targets in ESCs, the list of Myc binding sites is largely incomplete due to the low sensitivity and specificity of the antibodies available. To systematically identify Myc binding sites in mouse ESCs, we used a stringent streptavidin-based genome-wide chromatin immunoprecipitation (ChIP-Seq) approach with biotin-tagged Myc (Bio-Myc) as well as a ChIP-Seq of the Myc binding partner Max. This analysis identified 4325 Myc binding sites, of which 2885 were newly identified. The identified sites overlap with more than 85% of the Max binding sites and are enriched for H3K4me3-positive promoters and active enhancers. Remarkably, this analysis unveils that Myc/Max regulates chromatin modifiers and transcriptional regulators involved in stem cell self-renewal linking the Myc-centered network with the Polycomb and the Core networks. These results provide insights into the contribution of Myc and Max in maintaining stem cell self-renewal and keeping these cells in an undifferentiated state.
format	article
author	Anna Krepelova Francesco Neri Mara Maldotti Stefania Rapelli Salvatore Oliviero
author_facet	Anna Krepelova Francesco Neri Mara Maldotti Stefania Rapelli Salvatore Oliviero
author_sort	Anna Krepelova
title	Myc and max genome-wide binding sites analysis links the Myc regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells.
title_short	Myc and max genome-wide binding sites analysis links the Myc regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells.
title_full	Myc and max genome-wide binding sites analysis links the Myc regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells.
title_fullStr	Myc and max genome-wide binding sites analysis links the Myc regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells.
title_full_unstemmed	Myc and max genome-wide binding sites analysis links the Myc regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells.
title_sort	myc and max genome-wide binding sites analysis links the myc regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/95ef5c7e72b54ffdab9f00496f497130
work_keys_str_mv	AT annakrepelova mycandmaxgenomewidebindingsitesanalysislinksthemycregulatorynetworkwiththepolycombandthecorepluripotencynetworksinmouseembryonicstemcells AT francesconeri mycandmaxgenomewidebindingsitesanalysislinksthemycregulatorynetworkwiththepolycombandthecorepluripotencynetworksinmouseembryonicstemcells AT maramaldotti mycandmaxgenomewidebindingsitesanalysislinksthemycregulatorynetworkwiththepolycombandthecorepluripotencynetworksinmouseembryonicstemcells AT stefaniarapelli mycandmaxgenomewidebindingsitesanalysislinksthemycregulatorynetworkwiththepolycombandthecorepluripotencynetworksinmouseembryonicstemcells AT salvatoreoliviero mycandmaxgenomewidebindingsitesanalysislinksthemycregulatorynetworkwiththepolycombandthecorepluripotencynetworksinmouseembryonicstemcells
_version_	1718421697674084352

Myc and max genome-wide binding sites analysis links the Myc regulatory network with the polycomb and the core pluripotency networks in mouse embryonic stem cells.

Ejemplares similares