Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds

Abstract The use of plant-based biomaterials for tissue engineering has recently generated interest as plant decellularization produces biocompatible scaffolds which can be repopulated with human cells. The predominant approach for vegetal decellularization remains serial chemical processing. Howeve...

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Autores principales:	Ashlee F. Harris, Jerome Lacombe, Sumedha Liyanage, Margaret Y. Han, Emily Wallace, Sophia Karsunky, Noureddine Abidi, Frederic Zenhausern
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/95f8cc58568349f89d38cfee86920dba
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spelling	oai:doaj.org-article:95f8cc58568349f89d38cfee86920dba2021-12-02T14:26:55ZSupercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds10.1038/s41598-021-83250-92045-2322https://doaj.org/article/95f8cc58568349f89d38cfee86920dba2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83250-9https://doaj.org/toc/2045-2322Abstract The use of plant-based biomaterials for tissue engineering has recently generated interest as plant decellularization produces biocompatible scaffolds which can be repopulated with human cells. The predominant approach for vegetal decellularization remains serial chemical processing. However, this technique is time-consuming and requires harsh compounds which damage the resulting scaffolds. The current study presents an alternative solution using supercritical carbon dioxide (scCO2). Protocols testing various solvents were assessed and results found that scCO2 in combination with 2% peracetic acid decellularized plant material in less than 4 h, while preserving plant microarchitecture and branching vascular network. The biophysical and biochemical cues of the scCO2 decellularized spinach leaf scaffolds were then compared to chemically generated scaffolds. Data showed that the scaffolds had a similar Young’s modulus, suggesting identical stiffness, and revealed that they contained the same elements, yet displayed disparate biochemical signatures as assessed by Fourier-transform infrared spectroscopy (FTIR). Finally, human fibroblast cells seeded on the spinach leaf surface were attached and alive after 14 days, demonstrating the biocompatibility of the scCO2 decellularized scaffolds. Thus, scCO2 was found to be an efficient method for plant material decellularization, scaffold structure preservation and recellularization with human cells, while performed in less time (36 h) than the standard chemical approach (170 h).Ashlee F. HarrisJerome LacombeSumedha LiyanageMargaret Y. HanEmily WallaceSophia KarsunkyNoureddine AbidiFrederic ZenhausernNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ashlee F. Harris Jerome Lacombe Sumedha Liyanage Margaret Y. Han Emily Wallace Sophia Karsunky Noureddine Abidi Frederic Zenhausern Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds
description	Abstract The use of plant-based biomaterials for tissue engineering has recently generated interest as plant decellularization produces biocompatible scaffolds which can be repopulated with human cells. The predominant approach for vegetal decellularization remains serial chemical processing. However, this technique is time-consuming and requires harsh compounds which damage the resulting scaffolds. The current study presents an alternative solution using supercritical carbon dioxide (scCO2). Protocols testing various solvents were assessed and results found that scCO2 in combination with 2% peracetic acid decellularized plant material in less than 4 h, while preserving plant microarchitecture and branching vascular network. The biophysical and biochemical cues of the scCO2 decellularized spinach leaf scaffolds were then compared to chemically generated scaffolds. Data showed that the scaffolds had a similar Young’s modulus, suggesting identical stiffness, and revealed that they contained the same elements, yet displayed disparate biochemical signatures as assessed by Fourier-transform infrared spectroscopy (FTIR). Finally, human fibroblast cells seeded on the spinach leaf surface were attached and alive after 14 days, demonstrating the biocompatibility of the scCO2 decellularized scaffolds. Thus, scCO2 was found to be an efficient method for plant material decellularization, scaffold structure preservation and recellularization with human cells, while performed in less time (36 h) than the standard chemical approach (170 h).
format	article
author	Ashlee F. Harris Jerome Lacombe Sumedha Liyanage Margaret Y. Han Emily Wallace Sophia Karsunky Noureddine Abidi Frederic Zenhausern
author_facet	Ashlee F. Harris Jerome Lacombe Sumedha Liyanage Margaret Y. Han Emily Wallace Sophia Karsunky Noureddine Abidi Frederic Zenhausern
author_sort	Ashlee F. Harris
title	Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds
title_short	Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds
title_full	Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds
title_fullStr	Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds
title_full_unstemmed	Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds
title_sort	supercritical carbon dioxide decellularization of plant material to generate 3d biocompatible scaffolds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/95f8cc58568349f89d38cfee86920dba
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Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds

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